Human embryonic development: from peri-implantation to gastrulation DOI
Jinglei Zhai, Zhenyu Xiao, Yiming Wang

et al.

Trends in Cell Biology, Journal Year: 2021, Volume and Issue: 32(1), P. 18 - 29

Published: Aug. 17, 2021

Language: Английский

Capturing Cardiogenesis in Gastruloids DOI Creative Commons
Giuliana Rossi, Nicolas Broguière, Matthew Miyamoto

et al.

Cell stem cell, Journal Year: 2020, Volume and Issue: 28(2), P. 230 - 240.e6

Published: Nov. 10, 2020

Language: Английский

Citations

232
Embryo model completes gastrulation to neurulation and organogenesis DOI Creative Commons
Gianluca Amadei, Charlotte E. Handford, Chengxiang Qiu

et al.

Nature, Journal Year: 2022, Volume and Issue: 610(7930), P. 143 - 153

Published: Aug. 25, 2022

Abstract Embryonic stem (ES) cells can undergo many aspects of mammalian embryogenesis in vitro 1–5 , but their developmental potential is substantially extended by interactions with extraembryonic cells, including trophoblast (TS) endoderm (XEN) and inducible XEN (iXEN) 6–11 . Here we assembled cell-derived embryos from mouse ES TS iXEN showed that they recapitulate the development whole natural embryo utero up to day 8.5 post-fertilization. Our model displays headfolds defined forebrain midbrain regions develops a beating heart-like structure, trunk comprising neural tube somites, tail bud containing neuromesodermal progenitors, gut tube, primordial germ cells. This complete within an yolk sac initiates blood island development. Notably, demonstrate neurulating Pax6 -knockout aggregated wild-type recapitulates ventral domain expansion occurs natural, ubiquitous embryos. Thus, these embryoids are powerful for dissecting roles diverse cell lineages genes results self-organization ability two types reconstitute through beyond gastrulation neurulation early organogenesis.

Language: Английский

Citations

192
Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs DOI Creative Commons
Shadi Tarazi, Alejandro Aguilera-Castrejon, Carine Joubran

et al.

Cell, Journal Year: 2022, Volume and Issue: 185(18), P. 3290 - 3306.e25

Published: Aug. 2, 2022

In vitro cultured stem cells with distinct developmental capacities can contribute to embryonic or extraembryonic tissues after microinjection into pre-implantation mammalian embryos. However, whether independently give rise entire gastrulating embryo-like structures and compartments remains unknown. Here, we adapt a recently established platform for prolonged ex utero growth of natural embryos generate mouse post-gastrulation synthetic whole embryo models (sEmbryos), both compartments, starting solely from naive ESCs. This was achieved by co-aggregating non-transduced ESCs, ESCs transiently expressing Cdx2 Gata4 promote their priming toward trophectoderm primitive endoderm lineages, respectively. sEmbryos adequately accomplish gastrulation, advance through key milestones, develop organ progenitors within complex similar E8.5 stage Our findings highlight the plastic potential pluripotent self-organize functionally reconstitute model beyond gastrulation.

Language: Английский

Citations

167
Reconstructing aspects of human embryogenesis with pluripotent stem cells DOI Creative Commons
Berna Sözen, Victoria Jorgensen, Bailey A. T. Weatherbee

et al.

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: Sept. 21, 2021

Abstract Understanding human development is of fundamental biological and clinical importance. Despite its significance, mechanisms behind embryogenesis remain largely unknown. Here, we attempt to model early embryo with expanded pluripotent stem cells (EPSCs) in 3-dimensions. We define a protocol that allows us generate self-organizing cystic structures from EPSCs display some hallmarks embryogenesis. These mimic polarization cavitation characteristic pre-implantation leading blastocyst morphology formation the transition post-implantation-like organization upon extended culture. Single-cell RNA sequencing these reveals subsets bearing resemblance epiblast, hypoblast trophectoderm lineages. Nevertheless, significant divergences natural blastocysts persist key markers, signalling pathways point towards ways which transcriptional-level cell identities may diverge models embryo. Thus, this platform provides insights into design

Language: Английский

Citations

147
Generation of extracellular morphogen gradients: the case for diffusion DOI
Kristina S. Stapornwongkul, Jean‐Paul Vincent

Nature Reviews Genetics, Journal Year: 2021, Volume and Issue: 22(6), P. 393 - 411

Published: March 25, 2021

Language: Английский

Citations

108
Periodic formation of epithelial somites from human pluripotent stem cells DOI Creative Commons
Marina Sanaki-Matsumiya, Mitsuhiro Matsuda, Nicola Gritti

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: April 28, 2022

Abstract During embryonic development, epithelial cell blocks called somites are periodically formed according to the segmentation clock, becoming foundation for segmental pattern of vertebral column. The process somitogenesis has recently been recapitulated with murine and human pluripotent stem cells. However, an in vitro model coupled clock epithelialization is still missing. Here, we report generation somitoids, organoids that form pairs somite-like structures. Somitoids display clear oscillations coincide presomitic mesoderm. resulting show anterior-posterior apical-basal polarities. Matrigel essential but dispensable differentiation into somite size rather constant, irrespective initial number. amount WNT signaling instructs proportion mesodermal lineages somitoids. provide a novel platform study somitogenesis.

Language: Английский

Citations

105
Reconstituting human somitogenesis in vitro DOI

Yoshihiro Yamanaka,

Sofiane Hamidi,

Kumiko Yoshioka-Kobayashi

et al.

Nature, Journal Year: 2022, Volume and Issue: 614(7948), P. 509 - 520

Published: Dec. 21, 2022

Language: Английский

Citations

86
Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development DOI Creative Commons

Kasey Y.C. Lau,

Hernan Rubinstein,

Carlos W. Gantner

et al.

Cell stem cell, Journal Year: 2022, Volume and Issue: 29(10), P. 1445 - 1458.e8

Published: Sept. 8, 2022

Several in vitro models have been developed to recapitulate mouse embryogenesis solely from embryonic stem cells (ESCs). Despite mimicking many aspects of early development, they fail capture the interactions between and extraembryonic tissues. To overcome this difficulty, we a ESC-based model that reconstitutes pluripotent ESC lineage two lineages post-implantation embryo by transcription-factor-mediated induction. This unified recapitulates developmental events day 5.5 8.5, including gastrulation; formation anterior-posterior axis, brain, beating heart structure; development tissues, yolk sac chorion. Comparing single-cell RNA sequencing individual structures with time-matched natural embryos identified remarkably similar transcriptional programs across but also showed when where diverges program. Our findings demonstrate an extraordinary plasticity ESCs self-organize generate whole-embryo-like structure.

Language: Английский

Citations

84
Reconstruction and deconstruction of human somitogenesis in vitro DOI
Yuchuan Miao, Yannis Djeffal, Alessandro De Simone

et al.

Nature, Journal Year: 2022, Volume and Issue: 614(7948), P. 500 - 508

Published: Dec. 21, 2022

Language: Английский

Citations

78
Modeling post-implantation stages of human development into early organogenesis with stem-cell-derived peri-gastruloids DOI Creative Commons
Lizhong Liu, Seiya Oura,

Zachary Markham

et al.

Cell, Journal Year: 2023, Volume and Issue: 186(18), P. 3776 - 3792.e16

Published: July 20, 2023

Language: Английский

Citations

68