Ganoderic acid A ameliorates depressive-like behaviors in CSDS mice: Insights from proteomic profiling and molecular mechanisms

Journal of Affective Disorders, Journal Year: 2024, Volume and Issue: 358, P. 270 - 282

Published: May 7, 2024

Language: Английский

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Understanding the molecular diversity of synapses

Nature reviews. Neuroscience, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 5, 2024

Language: Английский

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Astrocyte-secreted factors modulate synaptic protein synthesis as revealed by puromycin labeling of isolated synaptosomes

Frontiers in Molecular Neuroscience, Journal Year: 2025, Volume and Issue: 18

Published: Feb. 20, 2025

The synaptic proteome can be shaped by proteins transported from the neuronal soma and/or mRNAs that are delivered to synapses where locally synthesized. This last mechanism is known as local translation. Local translation has been extensively studied in neurons physiological conditions and, more recently, neurological disorders, which transcriptomes and translatomes become dysregulated. It widely believed neurons, main source of localized transcripts primarily regulated neuron itself. However, we wondered whether glial cells, especially astrocytes, could contribute modulation protein synthesis. To address this question, compared levels produced compartments neuron-astrocyte co-cultures using modified Boyden chambers or astrocyte-conditioned medium. We developed a methodology measure synthesis puromycin labeling isolated synaptosomes devoid somatic input. Our results show enhanced retained when cultured presence astrocytes response Puromycin coupled with proximity ligation identified Rpl26 one whose astrocyte-secreted factors. thus unravel contribution glia point previously unexplored extra layer complexity regulation neurons.

Language: Английский

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Regulation of PV interneuron plasticity by neuropeptide-encoding genes

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Neuronal activity must be regulated in a narrow permissive band for the proper operation of neural networks. Changes synaptic connectivity and network activity-for example, during learning-might disturb this balance, eliciting compensatory mechanisms to maintain function1-3. In neocortex, excitatory pyramidal cells inhibitory interneurons exhibit robust forms stabilizing plasticity. However, although neuronal plasticity has been thoroughly studied cells4-8, little is known about how adapt persistent changes their activity. Here we describe critical cellular process through which cortical parvalbumin-expressing (PV+) levels. We found that individual PV+ drive bidirectional adjustments number strength synapses received by these cells, specifically from other interneurons. High-throughput profiling ribosome-associated mRNA revealed increasing interneuron leads upregulation two genes encoding multiple secreted neuropeptides: Vgf Scg2. Functional experiments demonstrated VGF critically required activity-dependent scaling onto Our findings reveal an instructive role neuropeptide-encoding regulating connections among adult mouse neocortex.

Language: Английский

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Exploring Neuregulin3: From physiology to pathology, a novel target for rational drug design

Biochemical Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 116964 - 116964

Published: May 1, 2025

Language: Английский

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