Cell Reports,
Journal Year:
2023,
Volume and Issue:
42(12), P. 113467 - 113467
Published: Nov. 17, 2023
The
hippocampus
is
broadly
impacted
by
neuromodulations.
However,
how
neuropeptides
shape
the
function
of
and
related
spatial
learning
memory
remains
unclear.
Here,
we
discover
crucial
role
cholecystokinin
(CCK)
in
heterosynaptic
neuromodulation
from
medial
entorhinal
cortex
(MEC)
to
hippocampus.
Systematic
knockout
CCK
gene
impairs
CA3-CA1
LTP
space-related
performance.
MEC
provides
most
CCK-positive
neurons
projecting
hippocampal
region,
which
potentiates
long-term
plasticity
heterosynaptically
a
frequency-
NMDA
receptor
(NMDAR)-dependent
manner.
Selective
inhibition
CCKergic
or
downregulation
their
mRNA
levels
also
formation
animals'
performance
water
maze.
This
excitatory
extrahippocampal
projection
releases
upon
high-frequency
excitation
active
during
animal
exploration.
Our
results
reveal
critical
projections
bridging
intra-
circuitry
at
electrophysiological
behavioral
levels.
Nature,
Journal Year:
2024,
Volume and Issue:
627(8002), P. 149 - 156
Published: Feb. 28, 2024
Abstract
The
glymphatic
movement
of
fluid
through
the
brain
removes
metabolic
waste
1–4
.
Noninvasive
40
Hz
stimulation
promotes
neural
activity
in
multiple
regions
and
attenuates
pathology
mouse
models
Alzheimer’s
disease
5–8
Here
we
show
that
multisensory
gamma
influx
cerebrospinal
efflux
interstitial
cortex
5XFAD
model
disease.
Influx
was
associated
with
increased
aquaporin-4
polarization
along
astrocytic
endfeet
dilated
meningeal
lymphatic
vessels.
Inhibiting
clearance
abolished
removal
amyloid
by
stimulation.
Using
chemogenetic
manipulation
a
genetically
encoded
sensor
for
neuropeptide
signalling,
found
vasoactive
intestinal
peptide
interneurons
facilitate
regulating
arterial
pulsatility.
Our
findings
establish
novel
mechanisms
recruit
system
to
remove
amyloid.
Nature Neuroscience,
Journal Year:
2024,
Volume and Issue:
27(9), P. 1844 - 1857
Published: July 15, 2024
Abstract
Neuropeptides
are
ubiquitous
in
the
nervous
system.
Research
into
neuropeptides
has
been
limited
by
a
lack
of
experimental
tools
that
allow
for
precise
dissection
their
complex
and
diverse
dynamics
circuit-specific
manner.
Opioid
peptides
modulate
pain,
reward
aversion
as
such
have
high
clinical
relevance.
To
illuminate
spatiotemporal
endogenous
opioid
signaling
brain,
we
developed
class
genetically
encoded
fluorescence
sensors
based
on
kappa,
delta
mu
receptors:
κLight,
δLight
µLight,
respectively.
We
characterized
pharmacological
profiles
these
mammalian
cells
dissociated
neurons.
used
κLight
to
identify
electrical
stimulation
parameters
trigger
release
scale
dynorphin
volume
transmission
brain
slices.
Using
vivo
fiber
photometry
mice,
demonstrated
utility
detecting
optogenetically
driven
observed
differential
response
fearful
rewarding
conditions.
Acta Physiologica,
Journal Year:
2025,
Volume and Issue:
241(2)
Published: Jan. 13, 2025
Somatostatin
from
pancreatic
δ-cells
is
a
paracrine
regulator
of
insulin
and
glucagon
secretion,
but
the
release
kinetics
whether
secretion
altered
in
diabetes
unclear.
This
study
aimed
to
improve
understanding
somatostatin
by
developing
tool
for
real-time
detection
individual
islets.
Reporter
cells
responding
with
cytoplasmic
Ca2+
concentration
([Ca2+]i)
changes
were
generated
co-expressing
receptor
SSTR2,
G-protein
Gα15
fluorescent
sensor
HeLa
cells.
induced
dose-dependent
[Ca2+]i
increases
reporter
half-maximal
maximal
effects
at
1.6
±
0.4
~30
nM,
respectively.
Mouse
human
islets
cell
elevations
that
inhibited
SSTR2
antagonist
CYN154806.
Depolarization
high
K+,
KATP
channel
blockade
or
increasing
glucose
3
11
mM
evoked
concomitant
Exposure
glucagon,
GLP-1
ghrelin
also
triggered
responses,
whereas
little
effect
was
obtained
islet
exposure
insulin,
glutamate,
GABA
urocortin-3.
Islets
type
2
diabetic
donors
higher
responses
after
K+
depolarization
compared
non-diabetic
islets,
although
fewer
identified
immunostaining.
Type
associated
hypersecretion
somatostatin,
which
has
implications
regulation
secretion.
The
new
assay
single-islet
holds
promise
further
studies
physiology
pathophysiology.
Nature Communications,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: Jan. 18, 2025
The
co-existence
and
co-transmission
of
neuropeptides
small
molecule
neurotransmitters
within
individual
neuron
represent
a
fundamental
characteristic
observed
across
various
species.
However,
the
differences
regarding
their
in
vivo
spatiotemporal
dynamics
underlying
molecular
regulation
remain
poorly
understood.
Here,
we
develop
GPCR-activation-based
(GRAB)
sensor
for
detecting
short
neuropeptide
F
(sNPF)
with
high
sensitivity
resolution.
Furthermore,
investigate
between
sNPF
acetylcholine
(ACh)
from
same
neurons.
Interestingly,
our
findings
reveal
distinct
release
ACh.
Notably,
results
indicate
that
synaptotagmins
(Syt)
are
involved
these
two
processes,
as
Syt7
Sytα
release,
while
Syt1
ACh
release.
Thus,
this
high-performance
GRAB
provides
robust
tool
studying
shedding
insights
into
unique
distinguish
neurotransmitters.
Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
34(25)
Published: Feb. 16, 2024
Abstract
Transition
metal
sulfides
have
garnered
significant
interest
in
the
realm
of
photoelectrochemical
(PEC)
sensors
due
to
their
remarkable
optical
and
electrical
properties.
However,
intrinsic
UV–vis
adsorption
electron‐donor
consumption
severely
hinder
nonirritating
situ
epidermal
PEC
applications.
Herein,
a
NIR‐808
nm
light‐excited
heterojunction,
phosphomolybdic
acid‐encapsulated
ytterbium‐doped
bismuth
sulfide
(Yb‐Bi
2
S
3
/PMA),
is
synthesized
by
hydrothermal
method
for
self‐sufficient
electron
donor‐based
sensing.
Further,
Yb‐Bi
/PMA
heterojunction
decorated
on
flexible
integrated
laser‐induced
graphene
electrode
arrays,
providing
ultrafast
charge
carrier
transfer
capacity
robust
mechanical
durability
(300
bending
cycles)
subsequent
wearable
As
showcase,
aptamer
molecular
recognition‐based
sensor
sweat
trace
Neuropeptide
Y
(NPY,
stress
or
depression
biomarker)
presents
an
ultra‐low
detection
limit
(0.39
fM)
good
anti‐interference
ability.
Moreover,
integration
aptasensor
with
signal
processing
wireless
communication
facilitates
analysis
NPY
dynamics
various
real
scenarios
(feeding
behaviors,
physiological
stress,
circadian
rhythm).
More
broadly,
this
research
establishes
foundation
NIR‐excitable
semiconductors
equipped
donor
capabilities,
unlocking
potential
ultrasensitive
sensing
Science,
Journal Year:
2024,
Volume and Issue:
385(6716)
Published: Sept. 26, 2024
To
determine
how
neuronal
circuits
encode
and
drive
behavior,
it
is
often
necessary
to
measure
manipulate
different
aspects
of
neurochemical
signaling
in
awake
animals.
Optogenetics
calcium
sensors
have
paved
the
way
for
these
types
studies,
allowing
perturbation
readout
spiking
activity
within
genetically
defined
cell
types.
However,
methods
lack
ability
further
disentangle
roles
individual
neuromodulator
neuropeptides
on
behavior.
We
review
recent
advances
chemical
biology
tools
that
enable
precise
spatiotemporal
monitoring
control
over
neuroeffectors
their
receptors
vivo.
also
highlight
discoveries
enabled
by
such
tools,
revealing
molecules
signal
across
timescales
learning,
orchestrate
behavioral
changes,
modulate
circuit
activity.
