Selective activation of naïve B cells with unique epitope specificity shapes autoantibody formation in celiac disease DOI Creative Commons
Saykat Das, Jorunn Stamnæs, Lene S. Høydahl

et al.

Journal of Autoimmunity, Journal Year: 2024, Volume and Issue: 146, P. 103241 - 103241

Published: May 15, 2024

Many antibody responses induced by infection, vaccination or autoimmunity show signs of convergence across individuals with epitope-dependent selection particular variable region gene segments and complementarity determining 3 properties. However, not much is known about the relationship between antigen-specific effector cells precursors present in naïve B-cell repertoire. Here, we sought to address this context celiac disease, where there a stereotyped autoantibody response against enzyme transglutaminase 2 (TG2). By generating TG2-specific monoclonal antibodies from both duodenal plasma circulating B cells, demonstrate discord repertoire that are selected for production. Hence, does fully reflect epitope preference usage observed memory cells. Instead, distinct target TG2 epitopes appear be selectively activated at expense TG2-binding targeting other epitopes.

Language: Английский

Adaptive immune receptor germline gene variation DOI Creative Commons
Martin Corcoran, Gunilla B. Karlsson Hedestam

Current Opinion in Immunology, Journal Year: 2024, Volume and Issue: 87, P. 102429 - 102429

Published: April 1, 2024

Recognition of antigens by T cell receptors (TCRs) and B (BCRs) is a key step in lymphocyte activation. cells mediate adaptive immune responses, which protect us against infections provide immunological memory, also, some instances, drive pathogenic responses autoimmune diseases. TCRs BCRs are encoded within loci that known to be genetically diverse. However, the extent functional impact this variation, both humans model animals used research, remain largely unknown. Experimental genetic evidence has demonstrated complementarity determining regions 1 2 (HCDR1 HCDR2), variable (V) region BCRs, also often make critical contacts with targeted antigen. Thus, knowledge about allelic variation genes encoding critically important for understanding outbred populations define responder non-responder phenotypes.

Language: Английский

Citations

3
Multimodal Affinity-Modulated Efficient Separation of Lysozyme with a Hierarchical MXene@MOF Hybrid Framework DOI
Jin Wang,

Yudan Shen,

Yuting Zhuang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(29), P. 12102 - 12111

Published: July 13, 2024

The development of abiotic protein affinity adsorbents remains challenging for the accurate acquisition and analysis specific species. Inspired by bacterial cell walls, a hierarchical hybrid framework is fabricated through oriented growth an Fe-based metal organic (MOF) on V

Language: Английский

Citations

3
Large-scale data mining of four billion human antibody variable regions reveals convergence between therapeutic and natural antibodies that constrains search space for biologics drug discovery DOI Creative Commons

Paweł Dudzic,

Dawid Chomicz,

Jarosław Kończak

et al.

mAbs, Journal Year: 2024, Volume and Issue: 16(1)

Published: June 6, 2024

The naïve human antibody repertoire has theoretical access to an estimated > 10

Language: Английский

Citations

3
Pan-viral serology uncovers distinct virome patterns as risk predictors of hepatocellular carcinoma and intrahepatic cholangiocarcinoma DOI Creative Commons

L. Whitney,

Limin Wang, Marshonna Forgues

et al.

Cell Reports Medicine, Journal Year: 2023, Volume and Issue: 4(12), P. 101328 - 101328

Published: Dec. 1, 2023

Highlights•Pan-viral serological profiling can distinguish liver cancer from healthy individuals•Exposure to hepatitis and non-hepatitis viruses associated with cancer•HCC viral score determine clinical risk mortality in at-risk populationSummaryThis study evaluates the pan-serological profiles of hepatocellular carcinoma (HCC) intrahepatic cholangiocarcinoma (iCCA) compared several diseased non-diseased control populations identify factors biomarkers cancer. We used phage immunoprecipitation sequencing, an anti-viral antibody screening method using a synthetic-phage-displayed human virome epitope library, screen patient serum samples for exposure over 1,280 strains pathogenic non-pathogenic viruses. Using machine learning methods develop HCC or iCCA score, we discovered that both scores were positively function markers two separate independent status. The predicted all-cause 8 years patients chronic disease at HCC, while status was not predictive survival. These results suggest infections may contribute development could be populations.Graphical abstract

Language: Английский

Citations

7
Selective activation of naïve B cells with unique epitope specificity shapes autoantibody formation in celiac disease DOI Creative Commons
Saykat Das, Jorunn Stamnæs, Lene S. Høydahl

et al.

Journal of Autoimmunity, Journal Year: 2024, Volume and Issue: 146, P. 103241 - 103241

Published: May 15, 2024

Many antibody responses induced by infection, vaccination or autoimmunity show signs of convergence across individuals with epitope-dependent selection particular variable region gene segments and complementarity determining 3 properties. However, not much is known about the relationship between antigen-specific effector cells precursors present in naïve B-cell repertoire. Here, we sought to address this context celiac disease, where there a stereotyped autoantibody response against enzyme transglutaminase 2 (TG2). By generating TG2-specific monoclonal antibodies from both duodenal plasma circulating B cells, demonstrate discord repertoire that are selected for production. Hence, does fully reflect epitope preference usage observed memory cells. Instead, distinct target TG2 epitopes appear be selectively activated at expense TG2-binding targeting other epitopes.

Language: Английский

Citations

2