bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 7, 2023
Abstract
The
3-D
structure
and
organization
of
type-1
collagen
protein
vasculature
for
a
set
ancient
permafrost
bones
is
extensively
documented
at
the
nanoscale
(up
to
150,000×
magnification)
first
time.
chemical
mapping
technique
ToF-SIMS
additionally
used
directly
localize
signal
these
structures;
C:N
isotope
measurements
are
also
reported
bulk
organic
bone
matrix.
These
analyses
test
hypothesis
that
biomolecular
histology
from
supports
their
taphonomic
classification
as
“subfossils”
rather
than
“fossils”.
Results
indicate
original
collagenous
scaffolding
still
present,
former
which
well-preserved,
thus
supporting
hypothesis.
This
study
taphonomically
classify
pre-Holocene
based
on
preserved
state
histology.
methods
can
be
readily
expanded
specimens
warmer
thermal
settings
earlier
geologic
strata.
Doing
so
has
potential
establish/formalize
what
point
been
truly
“fossilized”;
is,
when
it
transitioned
“subfossil”
status
being
true
“fossil”
bone.
will
elucidate
fossilization
process
vertebrates
lead
deeper
understanding
means
“fossil”.
iScience,
Journal Year:
2024,
Volume and Issue:
27(9), P. 110538 - 110538
Published: July 20, 2024
Fossil
bones
have
been
studied
by
paleontologists
for
centuries.
Despite
this,
empirical
knowledge
regarding
the
progression
of
biomolecular
(soft)
tissue
diagenesis
within
ancient
bone
is
limited;
this
particularly
case
specimens
spanning
Pleistocene
directly
into
pre-Ice
Age
strata.
A
nanoscopic
approach
reported
herein
that
facilitates
direct
imaging,
and
thus
observation,
soft
preservation
state.
Presented
data
include
first
extensive
(up
to
150,000×
magnification),
three-dimensional
(3D)
images
protein
vasculature;
chemical
signals
consistent
with
collagen
membrane
lipids,
respectively,
are
also
localized
these
structures.
These
findings
support
analyzed
permafrost
not
fully
fossilized
but
rather
represent
subfossil
as
they
preserve
an
underlying
framework.
Extension
methods
geologic
record
will
help
reveal
changes
tissues
undergo
during
fossilization
a
potential
proxy
screening
specimen
suitability
molecular
sequencing.
Trends in Microbiology,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 1, 2024
In
this
review,
we
delineate
the
unique
set
of
characteristics
associated
with
cryosphere
environments
(namely,
ice
and
permafrost)
which
present
both
challenges
opportunities
for
studying
ancient
environmental
microbiomes
(AEMs).
a
field
currently
reliant
on
several
assumptions,
discuss
theoretical
empirical
feasibility
recovering
microbial
nucleic
acids
(NAs)
from
permafrost
varying
degrees
antiquity.
We
also
summarize
contamination
control
best
practices
highlight
considerations
latest
approaches,
including
shotgun
metagenomics,
downstream
bioinformatic
authentication
approaches.
review
adoption
existing
software
provide
an
overview
more
recently
published
programs,
reference
to
their
suitability
AEM
studies.
Finally,
outstanding
likely
future
directions
research.
Nature,
Journal Year:
2024,
Volume and Issue:
636(8042), P. 296 - 298
Published: Dec. 10, 2024
DNA
recovered
from
ancient
remains
is
transforming
our
understanding
of
organisms
and
ecosystems
tens,
thousands
even
millions
years
ago
–
but
the
growing
volume
data
must
be
better
preserved.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 7, 2023
Abstract
The
3-D
structure
and
organization
of
type-1
collagen
protein
vasculature
for
a
set
ancient
permafrost
bones
is
extensively
documented
at
the
nanoscale
(up
to
150,000×
magnification)
first
time.
chemical
mapping
technique
ToF-SIMS
additionally
used
directly
localize
signal
these
structures;
C:N
isotope
measurements
are
also
reported
bulk
organic
bone
matrix.
These
analyses
test
hypothesis
that
biomolecular
histology
from
supports
their
taphonomic
classification
as
“subfossils”
rather
than
“fossils”.
Results
indicate
original
collagenous
scaffolding
still
present,
former
which
well-preserved,
thus
supporting
hypothesis.
This
study
taphonomically
classify
pre-Holocene
based
on
preserved
state
histology.
methods
can
be
readily
expanded
specimens
warmer
thermal
settings
earlier
geologic
strata.
Doing
so
has
potential
establish/formalize
what
point
been
truly
“fossilized”;
is,
when
it
transitioned
“subfossil”
status
being
true
“fossil”
bone.
will
elucidate
fossilization
process
vertebrates
lead
deeper
understanding
means
“fossil”.
