Dirigent isoflavene-forming PsPTS2: 3D structure, stereochemical, and kinetic characterization comparison with pterocarpan-forming PsPTS1 homolog in pea DOI Creative Commons
Qingyan Meng, Syed Moinuddin, Rhodesia M. Celoy

et al.

Journal of Biological Chemistry, Journal Year: 2024, Volume and Issue: 300(3), P. 105647 - 105647

Published: Jan. 18, 2024

Pea phytoalexins (–)-maackiain and (+)-pisatin have opposite C6a/C11a configurations, but biosynthetically how this occurs is unknown. dirigent-protein (DP) PsPTS2 generates 7,2ʹ-dihydroxy-4ʹ,5ʹ-methylenedioxyisoflav-3-ene (DMDIF), stereoselectivity towards four possible 7,2ʹ-dihydroxy-4ʹ,5ʹ-methylenedioxyisoflavan-4-ol (DMDI) stereoisomers was investigated. Stereoisomer configurations were determined using NMR spectroscopy, electronic circular dichroism, molecular orbital analyses. efficiently converted cis-(3R,4R)-DMDI into DMDIF 20-fold faster than the trans-(3R,4S)-isomer. The 4R-configured substrate's near β-axial OH orientation significantly enhanced its leaving group abilities in generating A-ring mono-quinone methide (QM), whereas 4S-isomer's α-equatorial-OH a poorer group. Docking simulations indicated that closest to Asp51, α-equatorial further away. Neither cis-(3S,4S)- nor trans-(3S,4R)-DMDIs substrates, even with former having C3/C4 stereochemistry as (+)-pisatin.PsPTS2 used cis-(3R,4R)-7,2′-dihydroxy-4′-methoxyisoflavan-4-ol [cis-(3R,4R)-DMI] give 2ʹ,7-dihydroxy-4ʹ-methoxyisoflav-3-ene (DMIF). DP homologs may exist licorice (Glycyrrhiza pallidiflora) tree legume Bolusanthus speciosus, DMIF both species.PsPTS1 utilized 2200-fold more cis-(3R,4R)-DMI. PsPTS1 also slowly trans-(3S,4R)-DMDI (+)-maackiain, reflecting better 4R configured group.PsPTS2 provisionally provide means enable differing C6a C11a (–)-maackiain, via identical DP-engendered mono-QM bound intermediate generation, which either re-aromatizes or intramolecularly cyclizes afford (–)-maackiain. Substrate docking indicate binds anti-configuration DMDIF, syn-configuration maackiain.

Language: Английский

How plants sense and respond to osmotic stress DOI Open Access
Bo Yu,

Dai‐Yin Chao,

Yang Zhao

et al.

Journal of Integrative Plant Biology, Journal Year: 2024, Volume and Issue: 66(3), P. 394 - 423

Published: Feb. 8, 2024

ABSTRACT Drought is one of the most serious abiotic stresses to land plants. Plants sense and respond drought stress survive under water deficiency. Scientists have studied how plants stress, or osmotic caused by drought, ever since Charles Darwin, gradually obtained clues about sensing signaling in Osmotic a physical stimulus that triggers many physiological changes at cellular level, including turgor, cell wall stiffness integrity, membrane tension, fluid volume, may some these stimuli trigger downstream responses. In this review, we emphasized potential movements organisms, compared putative signal inputs wall‐containing wall‐free prospected volume according advances plants, animals, yeasts, bacteria, summarized multilevel biochemical outputs, such as plasma nanodomain formation, permeability, root hydrotropism, halotropism, Casparian strip suberin lamellae, finally proposed hypothesis responses are likely be cocktail mediated multiple osmosensors. We also discussed core scientific questions, provided perspective future directions field, highlighted importance robust smart systems efficient source‐sink allocations for generating high‐yield stress‐resistant crops

Language: Английский

Citations

24
Lignin Biosynthesis and Its Diversified Roles in Disease Resistance DOI Open Access
Qing-Hu Ma

Genes, Journal Year: 2024, Volume and Issue: 15(3), P. 295 - 295

Published: Feb. 25, 2024

Lignin is complex, three-dimensional biopolymer existing in plant cell wall. biosynthesis increasingly highlighted because it closely related to the wide applications agriculture and industry productions, including pulping process, forage digestibility, bio-fuel, carbon sequestration. The functions of lignin

Language: Английский

Citations

22
Trichoderma-secreted anthranilic acid promotes lateral root development via auxin signaling and RBOHF-induced endodermal cell wall remodeling DOI Creative Commons

Yu Chen,

Yansong Fu,

Yanwei Xia

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(4), P. 114030 - 114030

Published: March 28, 2024

Trichoderma spp. have evolved the capacity to communicate with plants by producing various secondary metabolites (SMs). Nonhormonal SMs play important roles in plant root development, while specific from rhizosphere microbes and their underlying mechanisms control branching are still largely unknown. In this study, a compound, anthranilic acid (2-AA), is identified T. guizhouense NJAU4742 promote lateral development. Further studies demonstrate that 2-AA positively regulates auxin signaling transport canonical pathway. also partly rescues numbers of CASP1

Language: Английский

Citations

13
Peroxidase in plant defense: Novel insights for cadmium accumulation in rice (Oryza sativa L.) DOI
Jiahui Liu,

Yunxuan Lv,

Meng Li

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 474, P. 134826 - 134826

Published: June 5, 2024

Language: Английский

Citations

11
Top five unanswered questions in plant cell surface research DOI Creative Commons
Wout Boerjan, Vincent Burlat, Daniel J. Cosgrove

et al.

The Cell Surface, Journal Year: 2024, Volume and Issue: 11, P. 100121 - 100121

Published: Feb. 13, 2024

Plant cell wall researchers were asked their view on what the major unanswered questions are in field. This article summarises feedback that was received from them five questions. In this issue you can find equivalent syntheses for working bacterial, unicellular parasite and fungal systems.

Language: Английский

Citations

9
Grass lignin: biosynthesis, biological roles, and industrial applications DOI Creative Commons
Luigi M. Peracchi, Rahele Panahabadi,

Jaime Barros

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 23, 2024

Lignin is a phenolic heteropolymer found in most terrestrial plants that contributes an essential role plant growth, abiotic stress tolerance, and biotic resistance. Recent research grass lignin biosynthesis has differences compared to dicots such as Arabidopsis thaliana . For example, the prolific incorporation of hydroxycinnamic acids into secondary cell walls improve structural integrity vascular elements via covalent crosslinking. Conversely, fundamental monolignol chemistry conserves mechanisms translocation polymerization across phylum. Emerging evidence suggests compositions contribute periods often alter cereal hinder pathogenesis. This same recalcitrance also inhibits industrial valorization biomass, making alterations reductions field research. review presents update biosynthesis, translocation, polymerization, highlights how lignified development responses, briefly addresses genetic engineering strategies may benefit applications.

Language: Английский

Citations

9
Woody plant cell walls: Fundamentals and utilization DOI Creative Commons
Wei Li, Ying‐Chung Jimmy Lin, Ying‐Lan Chen

et al.

Molecular Plant, Journal Year: 2023, Volume and Issue: 17(1), P. 112 - 140

Published: Dec. 15, 2023

Cell walls in plants, particularly forest trees, are the major carbon sink of terrestrial ecosystem. Chemical and biosynthetic features plant cell were revealed early on, focusing mostly on herbaceous model species. Recent developments genomics, transcriptomics, epigenomics, transgenesis, associated analytical techniques enabling novel insights into formation woody walls. Here, we review multilevel regulation wall biosynthesis tree We highlight current approaches to engineering as potential feedstock for materials energy survey reported field tests such engineered transgenic trees. outline opportunities challenges future research better understand type biogenesis more efficient wood modification utilization biomaterials or enhanced capture storage.

Language: Английский

Citations

21
The OsDIR55 gene increases salt tolerance by altering the root diffusion barrier DOI
Baoping Xue, Wen Duan, Luping Gong

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: 118(5), P. 1550 - 1568

Published: Feb. 27, 2024

The increased soil salinity is becoming a major challenge to produce more crops and feed the growing population of world. In this study, we demonstrated that overexpression OsDIR55 gene enhances rice salt tolerance by altering root diffusion barrier. broadly expressed in all examined tissues organs with maximum expression levels at lignified regions roots. Salt stress upregulates an abscisic acid (ABA)-dependent manner. Loss-function compromised improved development CS barrier, manifested decreased width CS, respectively, ultimately affected permeability apoplastic barrier deficiency resulted Na

Language: Английский

Citations

8
Integrative Dissection of Lignin Composition in Tartary Buckwheat Seed Hulls for Enhanced Dehulling Efficiency DOI Creative Commons
Wenqi Yang, Haiyang Duan, Ke Yu

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(20)

Published: March 23, 2024

Abstract The rigid hull encasing Tartary buckwheat seeds necessitates a laborious dehulling process before flour milling, resulting in considerable nutrient loss. Investigation of lignin composition is pivotal understanding the structural properties tartary hulls, as key determinant rigidity plant cell walls, thus directly impacting process. Here, seed hulls from 274 accessions analyzed, unveiling unique chemotype primarily consisting G lignin, common feature gymnosperms. Furthermore, hardness showed strong negative correlation with S content. Genome‐wide detection selective sweeps uncovered that genes governing biosynthesis specifically two caffeic acid O‐methyltransferases (COMTs) and one ferulate 5‐hydroxylases, are selected during domestication. This likely contributed to increased content decreased more domesticated varieties. association studies identified robust associations between FtCOMT1 accumulation hull. Transgenic Arabidopsis comt1 plants expressing successfully reinstated content, confirming its conserved function across species. These findings provide valuable metabolic genetic insights for potential redesign hulls.

Language: Английский

Citations

6
Extracellular vesicles of Norway spruce contain precursors and enzymes for lignin formation and salicylic acid DOI Creative Commons
Santeri Kankaanpää, Enni Väisänen, Geert Goeminne

et al.

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 196(2), P. 788 - 809

Published: May 21, 2024

Abstract Lignin is a phenolic polymer in plants that rigidifies the cell walls of water-conducting tracheary elements and support-providing fibers stone cells. Different mechanisms have been suggested for transport lignin precursors to site lignification wall. Extracellular vesicle (EV)-enriched samples isolated from lignin-forming suspension culture Norway spruce (Picea abies L. Karst.) contained both metabolites enzymes related biosynthesis. Metabolomic analysis revealed mono-, di-, oligolignols EV isolates, as well carbohydrates amino acids. In addition, salicylic acid (SA) some proteins involved SA signaling were detected EV-enriched samples. A proteomic several laccases, peroxidases, β-glucosidases, putative dirigent proteins, wall-modifying enzymes, such glycosyl hydrolases, transglucosylase/hydrolases, expansins EVs. Our findings suggest EVs are transporting required polymerization spruce, radical coupling monolignols can occur these vesicles.

Language: Английский

Citations

6