Plasmids are anchored to inactive regions of eukaryotic chromosomes through a nucleosome signal

The EMBO Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Abstract Natural plasmids are common in prokaryotes, but few have been documented eukaryotes. The natural 2µ plasmid present the yeast Saccharomyces cerevisiae is one of these best-characterized exceptions. This highly stable genetic element has coexisted with its host for millions years, faithfully segregating at each cell division through a mechanism that remains unclear. Using proximity ligation methods (such as Hi-C, Micro-C) to map contacts between and chromosomes under dozens different biological conditions, we found tethered preferentially regions low transcriptional activity, often corresponding long, inactive genes. These do not depend on chromosome-structuring factors, such members structural maintenance chromosome complexes (SMC) nucleosome-encoded signal associated RNA Pol II depletion. They appear throughout cycle can be established within minutes. hitchhiking strategy may extend beyond plasmid/ S. pair, suggested by binding pattern eukaryotic Ddp5 along silent amoeba Dictyostelium discoideum .

Language: Английский

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La levure et l’ADN étranger

Pour la Science, Journal Year: 2025, Volume and Issue: nº 570(4), P. 84 - 87

Published: March 26, 2025

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