Characterization of an Ecdysone Oxidase from Plutella xylostella (L.) and Its Role in Bt Cry1Ac Resistance

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Understanding the molecular mechanisms underlying insect resistance to Bacillus thuringiensis (Bt) pesticidal proteins is crucial for sustainable pest management. Here, we found that downregulation of Plutella xylostella ecdysone oxidase gene (PxEO) in normal feeding stages contributes increased 20-hydroxyecdysone (20E) titer and mediates Bt Cry1Ac toxin. The PxEO was cloned its expression significantly downregulated midgut Bt-resistant Cry1Ac-selected P. xylostella. Silencing reduced susceptibility, closely linked protein metabolized (E) 20E vitro, reduction elevated titers activated MAPK-mediated trans-regulatory mechanism known directly cause phenotype. Together with our recently reported 20E-degrading glucose dehydrogenase, this finding highlights a robust, multipronged, approach developed by 20E-mediated defense against harmful agents.

Language: Английский

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Innovation and technology for achieving resilient and inclusive rural transformation

Global Food Security, Journal Year: 2025, Volume and Issue: 44, P. 100827 - 100827

Published: Jan. 26, 2025

Language: Английский

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Modelling and estimating pollen movement in oilseed rape (Brassica napus) at the landscape scale using genetic markers

Molecular Ecology, Journal Year: 2006, Volume and Issue: 16(3), P. 487 - 499

Published: Nov. 16, 2006

Understanding patterns of pollen movement at the landscape scale is important for establishing management rules following release genetically modified (GM) crops. We use here a mating model adapted to cultivated species estimate dispersal kernels from genotypes progenies male-sterile plants positioned different sampling sites within 10 x 10-km oilseed rape production area. Half clouds sampled by originated uncharacterized sources that could consist both large volunteer and feral populations, fields outside study The geometric kernel was most appropriate predict in It predicted much larger proportion long-distance pollination than previously fitted kernels. This best-fitting underestimated level differentiation among but its spatial structure. estimation method validated on simulated genotypic data, proved provide good estimates shape rate composition issued sources. best here, kernel, should now be integrated into models aim predicting gene flow level, particular between GM non-GM

Language: Английский

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High diversity of oilseed rape pollen clouds over an agro‐ecosystem indicates long‐distance dispersal

Molecular Ecology, Journal Year: 2005, Volume and Issue: 14(8), P. 2269 - 2280

Published: May 3, 2005

Abstract Estimating the frequency of long‐distance pollination is important in cultivated species, particularly to assess risk gene transfer following release genetically modified crops. For this purpose, we estimated diversity and origin fertilizing pollen a 10 × km French oilseed rape production area. First, cultivar grown each field was identified through surveys farmers using microsatellite markers. Examination seed set fields indicated high rates contamination (8.7%) from other sources (5%). Then, male‐sterile plants were scattered over study area their genotyped same Most local: 65% seeds had compatible sire closest field, i.e. at 50 or 300 m depending on site, but nearest found more than 1000 away for 13% seeds. To pollen, assigned putative siring cultivar. The observed then compared that predicted by simulations three empirical dispersal models with increasing proportion pollination. sensitive kernel used simulations, fatter‐tailed functions predicting higher diversities. consistent our data exponential function.

Language: Английский

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Exploring the potential role of EPSPS mutations for enhanced glyphosate resistance in Nicotiana tabacum

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 10, 2025

Glyphosate is a widely used non-selective, broad-spectrum, systemic herbicide by interfering with the biosynthesis of aromatic amino acids. However, emergence glyphosate-resistant weeds has driven need for enhanced resistance in crops. In this study, we engineered two mutant variants tobacco EPSPS gene through acid substitution (TIPS-NtEPSPS and P180S-NtEPSPS). These mutated genes were overexpressed under control CaMV35S promoters. Our results demonstrate that overexpression TIPS-NtEPSPS significantly enhances glyphosate tolerance, allowing plants to withstand up four times recommended dose without compromising their fitness. This research highlights potential improve tobacco, offering viable approach effective weed management.

Language: Английский

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Eco-bio-communitarianism: reimagining bioethics for sustainable health

BMC Global and Public Health, Journal Year: 2025, Volume and Issue: 3(1)

Published: Feb. 11, 2025

Language: Английский

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Rethinking progress: harmonizing the discourse on genetically modified crops

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 21, 2025

Genetically modified crops (GM crops) also known as biotech are that have been altered through genetic engineering techniques and under cultivation for approximately 28 years. By October 2024, over 30 nations approved the of GM crops. The global area utilized crop production has reached 206.3 million hectares. Despite substantial growth in these crops, debate continues between proponents opponents In this article, critical concerns common ground arguments both sides were described. main issues addressed include naturalness religious perspectives, beneficial aspects, safety issues, socio-economic impacts intellectual property rights. We argue classification unnatural is a claim lacks scientific reality. similar vein, comparing technology to act playing God inappropriate. Moreover, belief do not contribute yield improvements inconsistent with empirical evidence. Additionally, foods produced from unsafe human consumption holds unseen on ground. highlighted necessity implementing rights support seed developers limited duration without violating farmers’ conclusion, consumer right know what they eat, labeling food products fosters transparency enhance autonomy.

Language: Английский

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Potential Mechanisms Underlying the Minimal Impact of Cry1Ab1 Protein on Myzus persicae

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2924 - 2924

Published: March 24, 2025

Transgenic crops have been commercially cultivated for nearly three decades, leading to increasing concerns about their environmental safety, particularly effects on non-target organisms. This study investigated the underlying mechanisms behind lack of impact Cry1Ab1 protein Myzus persicae. The showed no significant survival and development M. Compared other Cry protein, fewer Cry1Ab1-binding proteins were identified including beta-actin, ATP synthase subunit alpha, GPN-loop GTPase 2. Transcriptomic analysis that a small set pathways, mainly involved in immune defense, temporarily enriched at 24 h after exposure while pathways 48 results suggest has transient minimal Further structural comparisons between (e.g., Cry1Ac) revealed differences Domain III, which likely reduced binding efficiency persicae's metabolism biological traits. provides valuable insights into molecular functional ineffectiveness persicae contributes safety evaluation Bt

Language: Английский

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Detoxification Strategy of Titanium Oxide Nanoparticles Driving Endogenous Molecules Metabolism to Modulate Atrazine Conversion in Lactuca sativa L.

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Nanoparticles (NPs) exhibit the potential to enhance plant tolerance organic pollutant stress, but how they drive endogenous molecules detoxify contaminants remains be further investigated. This study clarified modulatory mechanisms by which foliar or root application of biosynthesized titanium oxide NPs (g-nTiO2) alleviated atrazine (ATZ) toxicity Lactuca sativa L. Compared with ATZ-alone group, 10 mg/L g-nTiO2 intensified light-harvesting, photoelectron transfer, and reduced oxidative damage, thereby improving growth inducing metabolic reprogramming. Specifically, activated pathways related energy supply defense detoxification, while stabilizing membrane lipid nitrogen metabolism. Furthermore, modulation biomarkers involved in balancing cellular homeostasis stimulating ultimately boosted lettuce resistance ATZ physiological performance. Molecular docking analysis revealed that enhanced Phase II metabolism glutathione amino acids through increasing detoxification enzyme activities 23–44%, confirmed driving role alleviating phytotoxicity lettuce. Collectively, these findings provide a prospective nanoenabled strategy for mitigating crop sensitivity pesticide residues safe sustainable agricultural production.

Language: Английский

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Is co-existence and/or containment of genetically modified plants possible, and is it important?

New Zealand Journal of Agricultural Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 52

Published: March 31, 2025

Language: Английский

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