Reactive oxygen species signalling in plant stress responses

Nature Reviews Molecular Cell Biology, Journal Year: 2022, Volume and Issue: 23(10), P. 663 - 679

Published: June 27, 2022

Language: Английский

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Stress-induced reactive oxygen species compartmentalization, perception and signalling

Nature Plants, Journal Year: 2021, Volume and Issue: 7(4), P. 403 - 412

Published: April 12, 2021

Language: Английский

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Cysteine Oxidation in Proteins: Structure, Biophysics, and Simulation

Biochemistry, Journal Year: 2022, Volume and Issue: 61(20), P. 2165 - 2176

Published: Sept. 26, 2022

Cysteine side chains can exist in distinct oxidation states depending on the pH and redox potential of environment, cysteine plays important yet complex regulatory roles. Compared with effects post-translational modifications such as phosphorylation, to sulfenic, sulfinic, sulfonic acid protein structure function remain relatively poorly characterized. We present an analysis role reactivity a factor proteins, emphasizing interplay between electrostatics key determinants resulting state. A review current computational approaches suggests underdeveloped areas research for studying through molecular simulations.

Language: Английский

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Emerging roles of Aurora-A kinase in cancer therapy resistance

Acta Pharmaceutica Sinica B, Journal Year: 2023, Volume and Issue: 13(7), P. 2826 - 2843

Published: March 15, 2023

Aurora kinase A (Aurora-A), a serine/threonine kinase, plays pivotal role in various cellular processes, including mitotic entry, centrosome maturation and spindle formation. Overexpression or gene-amplification/mutation of Aurora-A occurs different types cancer, lung colorectal breast cancer. Alteration impacts multiple cancer hallmarks, especially, immortalization, energy metabolism, immune escape cell death resistance which are involved progression resistance. This review highlights the most recent advances oncogenic roles related hallmarks kinase-driving therapy resistance, chemoresistance (taxanes, cisplatin, cyclophosphamide), targeted (osimertinib, imatinib, sorafenib, etc.), endocrine (tamoxifen, fulvestrant) radioresistance. Specifically, mechanisms promote acquired through modulating DNA damage repair, feedback activation bypass pathways, to apoptosis, necroptosis autophagy, metastasis, stemness. Noticeably, our also summarizes promising synthetic lethality strategy for inhibitors RB1, ARID1A MYC gene mutation tumors, potential synergistic mTOR, PAK1, MDM2, MEK PD-L1 antibodies combined with targeting kinase. In addition, we discuss design development novel class precision medicine treatment.

Language: Английский

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A critical evaluation of protein kinase regulation by activation loop autophosphorylation

eLife, Journal Year: 2023, Volume and Issue: 12

Published: July 20, 2023

Phosphorylation of proteins is a ubiquitous mechanism regulating their function, localization, or activity. Protein kinases, enzymes that use ATP to phosphorylate protein substrates are, therefore, powerful signal transducers in eukaryotic cells. The phosphoryl-transfer universally conserved among which necessitates the tight regulation kinase activity for orchestration cellular processes with high spatial and temporal fidelity. In response stimulus, many kinases enhance own by autophosphorylating amino acid activation loop, but precisely how this reaction performed controversial. Classically, autophosphorylate loop are thought perform trans , mediated transient dimerization domains. However, motivated recently discovered cis -autophosphorylation autoinhibited we here review various mechanisms autoregulation have been proposed. We provide framework critically evaluating biochemical, kinetic, structural evidence autophosphorylation, share some thoughts on implications these within physiological signaling networks.

Language: Английский

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Phosphorylated transcription factor PuHB40 mediates ROS-dependent anthocyanin biosynthesis in pear exposed to high light

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(9), P. 3562 - 3583

Published: June 6, 2024

Abstract Plants are increasingly vulnerable to environmental stresses because of global warming and climate change. Stress-induced reactive oxygen species (ROS) accumulation results in plant cell damage, even death. Anthocyanins important antioxidants that scavenge ROS maintain homeostasis. However, the mechanism underlying ROS-induced anthocyanin is unclear. In this study, we determined HD-Zip I family member transcription factor PuHB40 mediates ROS-dependent biosynthesis under high-light stress pear (Pyrus ussuriensis). Specifically, induces PuMYB123-like–PubHLH3 complex for biosynthesis. The PuHB40-mediated transcriptional activation depends on its phosphorylation level, which regulated by protein phosphatase PP2A. Elevated content maintains high levels while also enhancing PuHB40-induced PuMYB123-like decreasing PuPP2AA2 expression, ultimately leading increased Our study reveals a pathway regulating pears, further clarifying abiotic stress-induced biosynthesis, may have implications improving tolerance.

Language: Английский

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Oxidative stress-mediated protein sulfenylation in human diseases: Past, present, and future

Redox Biology, Journal Year: 2024, Volume and Issue: 76, P. 103332 - 103332

Published: Aug. 30, 2024

Reactive Oxygen Species (ROS) refer to a variety of derivatives molecular oxygen that play crucial roles in regulating wide range physiological and pathological processes. Excessive ROS levels can cause oxidative stress, leading cellular damage even cell demise. However, moderately elevated mediate the post-translational modifications (oxPTMs) redox-sensitive proteins, thereby affecting protein functions various signaling pathways. Among oxPTMs, ROS-induced reversible sulfenylation represents initial form cysteine oxidation for sensing redox signaling. In this review, we will summarize discovery, chemical formation, detection approaches sulfenylation. addition, highlight recent findings diseases, including thrombotic disorders, diabetes, cardiovascular neurodegenerative cancer.

Language: Английский

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A mitotic NADPH upsurge promotes chromosome segregation and tumour progression in aneuploid cancer cells

Nature Metabolism, Journal Year: 2023, Volume and Issue: 5(7), P. 1141 - 1158

Published: June 22, 2023

Language: Английский

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A peroxiredoxin-P38 MAPK scaffold increases MAPK activity by MAP3K-independent mechanisms

Molecular Cell, Journal Year: 2023, Volume and Issue: 83(17), P. 3140 - 3154.e7

Published: Aug. 11, 2023

Peroxiredoxins (Prdxs) utilize reversibly oxidized cysteine residues to reduce peroxides and promote H2O2 signal transduction, including H2O2-induced activation of P38 MAPK. Prdxs form disulfide complexes with many proteins, multiple kinases involved in MAPK signaling. Here, we show that a genetically encoded fusion between Prdx is sufficient hyperactivate the kinase yeast human cells by mechanism does not require H2O2-sensing Prdx. We demonstrate P38-Prdx protein compensates for loss scaffold Mcs4 MAP3K activity, driving into mitosis. Based on our findings, propose formation Prdx-MAPK provides an alternative signaling platform MAPKK-MAPK The demonstration complex modify activity has broad implications peroxide-based transduction eukaryotes.

Language: Английский

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Navigating the redox landscape: reactive oxygen species in regulation of cell cycle

Redox Report, Journal Year: 2024, Volume and Issue: 29(1)

Published: July 7, 2024

Objectives: To advance our knowledge of disease mechanisms and therapeutic options, understanding cell cycle regulation is critical. Recent research has highlighted the importance reactive oxygen species (ROS) in regulation. Although excessive ROS levels can lead to age-related pathologies, also play an essential role normal cellular functions. Many regulatory proteins are affected by their redox status, but precise conditions under which promote or inhibit proliferation not fully understood.

Language: Английский

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