A single-cell atlas of circulating immune cells over the first 2 months of age in extremely premature infants DOI
Oluwabunmi Olaloye,

Weihong Gu,

Arne Gehlhaar

et al.

Science Translational Medicine, Journal Year: 2025, Volume and Issue: 17(788)

Published: March 5, 2025

Extremely premature infants (EPIs) who are born before 30 weeks of gestation susceptible to infection; however, the trajectory their peripheral immunity is poorly understood. Here, we undertook longitudinal analyses immune cells from 250 μl whole blood at 1 week, month, and 2 months 10 EPIs compared these with samples healthy adults preterm full-term cord samples. Single-cell suspensions individual were split perform single-cell RNA sequencing, T B cell receptor phosphoprotein mass cytometry. The trajectories circulating T, B, myeloid, natural killer in over first life distinct those infants. In EPIs, development rapidly progressed month life, an increase proportion naïve CD4+, regulatory, cycling cells, accompanied by greater STAT5 (signal transducer activator transcription 5) signaling. EPI memory CD4+ showed a helper (TH1) predominance TH2 skewing central memory-like infants, 2-month-old exhibited increased signatures activation differentiation. Both displayed interferon conclusion, demonstrated feasibility multiomic using minute amounts developed resource describing that suggested ongoing early life.

Language: Английский

A single-cell atlas of circulating immune cells over the first 2 months of age in extremely premature infants DOI
Oluwabunmi Olaloye,

Weihong Gu,

Arne Gehlhaar

et al.

Science Translational Medicine, Journal Year: 2025, Volume and Issue: 17(788)

Published: March 5, 2025

Extremely premature infants (EPIs) who are born before 30 weeks of gestation susceptible to infection; however, the trajectory their peripheral immunity is poorly understood. Here, we undertook longitudinal analyses immune cells from 250 μl whole blood at 1 week, month, and 2 months 10 EPIs compared these with samples healthy adults preterm full-term cord samples. Single-cell suspensions individual were split perform single-cell RNA sequencing, T B cell receptor phosphoprotein mass cytometry. The trajectories circulating T, B, myeloid, natural killer in over first life distinct those infants. In EPIs, development rapidly progressed month life, an increase proportion naïve CD4+, regulatory, cycling cells, accompanied by greater STAT5 (signal transducer activator transcription 5) signaling. EPI memory CD4+ showed a helper (TH1) predominance TH2 skewing central memory-like infants, 2-month-old exhibited increased signatures activation differentiation. Both displayed interferon conclusion, demonstrated feasibility multiomic using minute amounts developed resource describing that suggested ongoing early life.

Language: Английский

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