Prevalence and patterns of multidrug-resistant bacteria isolated from sputum samples of patients with bacterial pneumonia at a tertiary hospital in Tanzania DOI Creative Commons
Doreen Kamori, Dominic S Renatus,

Ambele M. Mwandigha

et al.

Bulletin of the National Research Centre/Bulletin of the National Research Center, Journal Year: 2024, Volume and Issue: 48(1)

Published: July 24, 2024

Abstract Background Antimicrobial resistance affects the treatment of several bacterial infections, including pneumonia. This subsequently increased morbidity and mortality rates patients with pneumonia, especially in resource-limited settings. In this study, we aimed to determine patterns multidrug-resistant (MDR) bacteria isolated from sputum samples pneumonia attending a tertiary hospital Tanzania. Methodology A retrospective cross-sectional study was conducted. It involved reviewing laboratory data information system at Muhimbili National Hospital The were previously processed using standard methods (culture, Gram staining, biochemical tests) isolate identify bacteria. At same time, antibiogram profiles determined antimicrobial susceptibility tests. Bacterial isolates that expressed MDR identified. Demographic collected patients' medical records. We used chi-square test factors associated MDR. p-value < 0.05 considered significant. Results retrieved analysed 169 records provisional clinical diagnosis confirmed microbiology laboratory. Nearly 98% adult patients. patients’ mean age 48.3 years 17.3 deviations. About 84% Gram-negative; most predominant Klebsiella pneumoniae (59/142; 41.5%). Gram-positive Staphylococcus aureus (25/27; 92.6%). Furthermore, 80 out (47.3%) MDR; (32.5%) predominant. addition, 50% methicillin resistance. highly observed admitted Intensive Care Unit (p 0.05). Conclusions Although our limited by variations number subjected antibiotic drugs lack on risk such as occupation, smoking history, marital status, high proportion is caused Gram-negative local setting. These results inform need improve infection prevention control measures hospitals reduce burden settings other similar

Language: Английский

Carbapenem-resistant Klebsiella pneumoniae : the role of plasmids in emergence, dissemination, and evolution of a major clinical challenge DOI Open Access
Vincenzo Di Pilato, Simona Pollini, Vivì Miriagou

et al.

Expert Review of Anti-infective Therapy, Journal Year: 2024, Volume and Issue: 22(1-3), P. 25 - 43

Published: Jan. 18, 2024

Introduction Klebsiella pneumoniae is a major agent of healthcare-associated infections and cause some community-acquired infections, including severe bacteremic associated with metastatic abscesses in liver other organs. Clinical relevance compounded by its outstanding propensity to evolve antibiotic resistance. In particular, the emergence dissemination carbapenem resistance K. has posed challenge due few residual treatment options, which have only recently been expanded new agents. The epidemiological success carbapenem-resistant (CR-Kp) mainly linked clonal lineages that produce carbapenem-hydrolyzing enzymes (carbapenemases) encoded plasmids.

Language: Английский

Citations

20
Clinical and laboratory insights into the threat of hypervirulent Klebsiella pneumoniae DOI

Xinhong Han,

Jiayao Yao,

Jintao He

et al.

International Journal of Antimicrobial Agents, Journal Year: 2024, Volume and Issue: 64(3), P. 107275 - 107275

Published: July 14, 2024

Language: Английский

Citations

12
Presence of hypervirulence-associated determinants in Klebsiella pneumoniae from hospitalised patients in Germany DOI Creative Commons

Anika Wahl,

Martin A. Fischer,

Kathleen Klaper

et al.

International Journal of Medical Microbiology, Journal Year: 2024, Volume and Issue: 314, P. 151601 - 151601

Published: Feb. 8, 2024

Klebsiella (K.) pneumoniae is a ubiquitous Gram-negative bacterium and common coloniser of animals humans. Today, K. one the most persistent nosocomial pathogens worldwide poses severe threat/burden to public health by causing urinary tract infections, pneumonia bloodstream infections. Affected infections are mainly immunocompromised individuals hospitalised patients. In recent years, new type has emerged associated with community-acquired such as pyogenic liver abscess in otherwise healthy therefore termed hypervirulent (hvKp). The aim this study was characterisation isolates properties hypervirulence from Germany. A set 62 potentially human patients compiled. Inclusion criteria were presence at least determinant that been previously hypervirulence: (I) clinical manifestation, (II) positive string test marker for hypermucoviscosity, (III) virulence genes rmpA and/or rmpA2 magA. Phenotypic included antimicrobial resistance testing broth microdilution. Whole genome sequencing (WGS) performed using Illumina® MiSeq, NextSeq investigate genetic repertoire multi-locus sequence types (ST), capsule (K), further collected isolates. For selected long-read applied plasmid sequences determinants compared. WGS analyses confirmed several signature hvKp. Among them, prevalent siderophore loci iuc ybt regulator rmpA2. dominant ST among hvKp ST395 K2 K5; both have described our data multidrug-resistant (MDR) ST23 K1 second abundant study; commonly hypervirulence. general, beta-lactams caused production extended-spectrum beta-lactamases (ESBL) carbapenemases observed frequently isolates, confirming threatening rise MDR-hvKp strains. Our results show strains carry present many years detection carbapenemase on same highly problematic requires intensified screening molecular surveillance. However, non-uniform definition complicates their detection. Testing hypermucoviscosity alone not specific enough identify Thus, we suggest classification should be only fulfil phenotypical (severe manifestations, hypermucoviscosity) but also two e.g. ybt,

Language: Английский

Citations

10
Hypervirulent Klebsiella pneumoniae: Epidemiology outside Asian countries, antibiotic resistance association, methods of detection and clinical management DOI Creative Commons
Silvia García-Cobos, Jesús Calatayud, Marı́a Pérez-Vázquez

et al.

Enfermedades Infecciosas y Microbiología Clínica, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

1
Comparative analysis of multidrug-resistant Klebsiella pneumoniae strains of food and human origin reveals overlapping populations DOI Creative Commons
Sergio Silva-Bea, Manuel Romero, Ana Parga

et al.

International Journal of Food Microbiology, Journal Year: 2024, Volume and Issue: 413, P. 110605 - 110605

Published: Jan. 23, 2024

Given the increasing incidence of multidrug-resistant (MDR) Klebsiella pneumoniae infections, it is great interest to investigate risk transmission associated with prevalence this pathogen. Some studies have described fresh raw poultry meat as a reservoir MDR K. pneumoniae, including clinically relevant sequence types (ST) and extended-spectrum β-lactamase (ESBL) strains, indicating possible consumer exposure. This study compared 47 strains from human clinical isolates assess similarities, analysis antimicrobial resistance profiles virulence factors involved in infection. In addition, several biofilm culture methods were evaluated for reproducible assessment formation strains. Globally, no association between strain origin STs, hypermucoviscosity, or serum could be found food origin, nor an AMR pattern, suggesting overlapping populations. We that LB supplemented glucose microaerobiosis was best discrimination condition active attachment cultivation model. The capacity strongly dependent on conditions, strain-specific response, but only minor increase levels recorded Our results suggest similar zoonosis potentially virulent foodborne previously observed E. coli also present high-priority further confirms pose consumers therefore pathogen should included surveillance pathogens high infections therapeutic failure.

Language: Английский

Citations

6
Genomic insights unveil the plasmid transfer mechanism and epidemiology of hypervirulent Klebsiella pneumoniae in Vietnam DOI Creative Commons
Quynh Nguyen,

Yen Thi Phuong Nguyen,

Tuyen Thanh Ha

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 17, 2024

Hypervirulent Klebsiella pneumoniae (hvKp) is a significant cause of severe invasive infections in Vietnam, yet data on its epidemiology, population structure and dynamics are scarce. We screened hvKp isolates from patients with bloodstream (BSIs) at tertiary infectious diseases hospital Vietnam healthy individuals, followed by whole genome sequencing plasmid analysis. Among 700 BSI-causing Kp strains, 100 (14.3%) were hvKp. Thirteen identified 350 rectal swabs adults; none 500 children. The genetically diverse, encompassing 17 sequence types (STs), predominantly ST23, ST86 ST65. the 113 isolates, 14 (12.6%) carried least one antimicrobial resistance (AMR) gene, largely mediated IncFII, IncR, IncA/C plasmids. Notably, acquisition AMR conjugative plasmids facilitated horizontal transfer non-conjugative virulence between K. strains. Phylogenetic analysis demonstrated BSIs human carriage clustered together, suggesting role intestinal transmission. Enhanced surveillance crucial to understand factors driving transmission for informing preventive measures. Furthermore, we advocate clinical use our molecular assay diagnosing guide effective management.

Language: Английский

Citations

6
Klebsiella pneumoniae sequence type 147: a high-risk clone increasingly associated with plasmids carrying both resistance and virulence elements DOI
Jane F. Turton,

Claire Perry,

Kim McGowan

et al.

Journal of Medical Microbiology, Journal Year: 2024, Volume and Issue: 73(4)

Published: April 17, 2024

Introduction. The first hybrid resistance/virulence plasmid, combining elements from virulence plasmids described in hypervirulent types of Klebsiella pneumoniae with those conjugative resistance plasmids, was an isolate sequence type (ST) 147 2016. Subsequently, this has been increasingly associated these plasmids. Hypothesis or gap statement. extent carriage virulence/resistance nosocomial isolates K. requires further investigation. Aim. To describe the occurrence among received by UK reference laboratory, particularly representatives ST147, and to compare their sequences. Methodology. Isolates laboratory during 2022 half 2023 ( n =1278) were screened for PCR detection rmpA / rmpA2 typed variable-number tandem repeat analysis. Twenty-nine ST147 (including a single-locus variant) seven hospital laboratories subjected long-read nanopore sequencing using high-accuracy q20 chemistry provide complete assemblies. Results. detected 110 isolates, which 59 belonged K1-ST23, K2-ST86 K2-ST65/375. Of remainder, formed largest group, 22 -positive (out 47 isolates). Representatives 19 laboratories, 10. Nanopore 29 divided them into no plasmid =12), non-New Delhi metallo-β-lactamase (NDM) =6) carrying bla NDM-5 =9) NDM-1 =2) These IncFIB(pNDM-Mar)/IncHI1B(pNDM-MAR) replicon types. Most non-NDM highly similar originally KpvST147L_NDM plasmid. Those one another previously ST383 carried extensive array genes. Comparison fully assembled chromosomes indicated multiple introductions hospitals. Conclusion. This study highlights high proportion that carry It is important be aware probability determinants consequent increased risk patients.

Language: Английский

Citations

5
Should multidrug resistant Klebsiella pneumoniae strains displaying hypervirulent traits be reclassified as either ultravirulent or supervirulent? DOI Creative Commons
Bruno Douradinha

Microbiological Research, Journal Year: 2023, Volume and Issue: 275, P. 127446 - 127446

Published: July 6, 2023

Language: Английский

Citations

12
Prevalence and molecular characteristics of colistin-resistant isolates among carbapenem-resistant Klebsiella pneumoniae in Central South China: a multicenter study DOI Creative Commons

Zijuan Jian,

Yanjun Liu, Zhiqian Wang

et al.

Annals of Clinical Microbiology and Antimicrobials, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 4, 2025

The emergence of colistin resistance in carbapenem-resistant Klebsiella pneumoniae (CRKP) is a significant public health concern, as has been the last resort for treating such infections. This study aimed to investigate prevalence and molecular characteristics colistin-resistant CRKP isolates Central South China. from twelve hospitals China were screened using broth microdilution. epidemiological characteristics, virulome, resistome, plasmid replicons two-component systems associated with explored by whole-genome sequencing. mgrB gene relative expression pmrC pmrK genes analyzed polymerase chain reaction (PCR) real-time quantitative PCR, respectively. bacterial virulence was evaluated through Galleria mellonella larvae infection model. Of 429 nonduplicate isolates, 26 (6.1%) they included eight clonal clusters. Six distinct sequence type (ST)-capsule loci (KL) types identified: ST11-KL64, ST11-KL47, ST963-KL16, ST307-KL102, ST751-KL64 ST5254-KL47. 88.5% (23/26) them found carry at least one carbapenemase gene, including blaKPC−2 (65.4%, 17/26) blaNDM−1 (7.7%, 2/26), well coharbouring (15.4%, 4/26). Diverse mutations resistance-related observed, inactivation insertions T157P deleterious mutation pmrB being detected 57.7% 42.3% In addition, novel mutation, R248P, crrB two ST11 isolates. presented an increase transcription, 69.2% had overexpression gene. All 16 ST11-KL64 isolate carried hypervirulence biomarkers showed high vivo. highlights presence different mechanisms belonging same clone identified multiple transmission clusters resistant globally high-risk ST307 clones, which proportion exhibited virulence. Consequently, it crucial enforce measures prevent ongoing spread resistance.

Language: Английский

Citations

0
Hypervirulent Klebsiella pneumoniae: Epidemiology outside Asian countries, antibiotic resistance association, methods of detection and clinical management DOI Creative Commons
Silvia García-Cobos, Jesús Calatayud, Marı́a Pérez-Vázquez

et al.

Enfermedades infecciosas y microbiologia clinica (English ed ), Journal Year: 2025, Volume and Issue: 43(2), P. 102 - 109

Published: Feb. 1, 2025

Two main Klebsiella pneumoniae pathotypes are of public health concern, classical K. (cKP), with high antibiotic resistance acquisition capacity, and hypervirulent (hvKP). The emergence antibiotic-resistant pneumoniae, especially carbapenem resistance, is worrisome require effective methods for detection treatment. Different evolutionary paths contribute to the hypervirulence commonly via plasmids by hvKP (CR-hvKP) or virulence CRKp (hv-CRKp). ST11-KL64 together blaKPC-2, most extended hv-CRKP lineage acquiring associated biomarkers, rmpA, rmpa2, iroBCDEN, iucABCDiutA, peg344. In addition ST11, other clones have been reported in Europe such as ST101, ST147 ST512, highlighting association OXA-48 NDM carbapenemases. Although still very rare Spain, cases increasing recent years, mainly due ST23-K1, ST380-K2 ST86-K2. Management infections requires active therapy based primarily on susceptibility patters site infection.

Language: Английский

Citations

0