Prevalence and patterns of multidrug-resistant bacteria isolated from sputum samples of patients with bacterial pneumonia at a tertiary hospital in Tanzania DOI Creative Commons
Doreen Kamori, Dominic S Renatus,

Ambele M. Mwandigha

et al.

Bulletin of the National Research Centre/Bulletin of the National Research Center, Journal Year: 2024, Volume and Issue: 48(1)

Published: July 24, 2024

Abstract Background Antimicrobial resistance affects the treatment of several bacterial infections, including pneumonia. This subsequently increased morbidity and mortality rates patients with pneumonia, especially in resource-limited settings. In this study, we aimed to determine patterns multidrug-resistant (MDR) bacteria isolated from sputum samples pneumonia attending a tertiary hospital Tanzania. Methodology A retrospective cross-sectional study was conducted. It involved reviewing laboratory data information system at Muhimbili National Hospital The were previously processed using standard methods (culture, Gram staining, biochemical tests) isolate identify bacteria. At same time, antibiogram profiles determined antimicrobial susceptibility tests. Bacterial isolates that expressed MDR identified. Demographic collected patients' medical records. We used chi-square test factors associated MDR. p-value < 0.05 considered significant. Results retrieved analysed 169 records provisional clinical diagnosis confirmed microbiology laboratory. Nearly 98% adult patients. patients’ mean age 48.3 years 17.3 deviations. About 84% Gram-negative; most predominant Klebsiella pneumoniae (59/142; 41.5%). Gram-positive Staphylococcus aureus (25/27; 92.6%). Furthermore, 80 out (47.3%) MDR; (32.5%) predominant. addition, 50% methicillin resistance. highly observed admitted Intensive Care Unit (p 0.05). Conclusions Although our limited by variations number subjected antibiotic drugs lack on risk such as occupation, smoking history, marital status, high proportion is caused Gram-negative local setting. These results inform need improve infection prevention control measures hospitals reduce burden settings other similar

Language: Английский

Multidrug-resistant hypervirulent Klebsiella pneumoniae : an evolving superbug DOI
Yuzhong Zheng,

Xiaojue Zhu,

Chao Ding

et al.

Future Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 13

Published: March 26, 2025

Multidrug-resistant hypervirulent Klebsiella pneumoniae (MDR-hvKP) combines high pathogenicity with multidrug resistance to become a new superbug. MDR-hvKP reports continue emerge, shattering the perception that K. (hvKP) strains are antibiotic sensitive. Patients infected have been reported in Asia, particularly China. Although hvKP can acquire drug genes, seems be more easily transformed from classical (cKP), which has strong gene uptake ability. To better understand biology of MDR-hvKP, this review discusses virulence factors, mechanisms, formation pathways, and identification MDR-hvKP. Given their destructive transmissible potential, continued surveillance these organisms enhanced control measures should prioritized.

Language: Английский

Citations

0
The Susceptibility of Klebsiella Pneumoniae Strains Isolated from COVID-19 Patients to Commercially Available Bacteriophage Medications DOI Creative Commons
Б. И. Асланов, A. Е. Goncharov, Sergey Konev

et al.

Antibiot Khimioter = Antibiotics and Chemotherapy, Journal Year: 2025, Volume and Issue: 69(11-12), P. 59 - 66

Published: April 19, 2025

Background . Superinfection caused by Klebsiella pneumoniae occupies a leading position in the structure of bacterial complications COVID-19 patients. The intensive circulation specialised hospitals has contributed to consolidation most clinically and epidemiologically important strains this pathogen, particular, representatives hypervirulent carbapenem-resistant clonal lines, which have not lost their relevance even post-pandemic period. use bacteriophages as therapeutic anti-epidemic agents seems justified given widespread multidrugresistant K. pneumoniae. Aim study To evaluate susceptibility associated with nosocomial infections patients polyvalent bacteriophage medications. Materials methods included 96 non-repeating isolated from clinical material admitted major hospital St. Petersburg severe moderate forms May 2020 January 2021. was assessed using spot test analysis. Commercially available preparations used for testing following: purified pyobacteriophage, sextaphage, pyobacteriophage. In order identify probable mechanisms resistance pneumoniae, nucleotide sequences genomes 6 pathogen belonging dominant genetic lines ST3, ST39, ST307, ST395, ST874 were studied. Results. Negative results tests observed 32.29% (95% CI=23.8–42.2) cases; general, proportion eligible treatment phage therapy 49% CI=39.2–58.8). Loci class 1 subtypes IV-A3 I-E, potentially CRISPR-Cas, identified genome studied strains, well number prophage bacteriophages. Conclusion demonstrated low activity medications against causing COVID-19. Increasing diversity active relevant clones can expand possibilities infections. rational containing these is possible within paradigm personalised therapy.

Language: Английский

Citations

0
Screening of Klebsiella pneumoniae subsp. pneumoniae Strains with Multi-Drug Resistance and Virulence Profiles Isolated from an Italian Hospital between 2020 and 2023 DOI Creative Commons
Valentina Dimartino, Carolina Venditti, Francesco Messina

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(6), P. 561 - 561

Published: June 15, 2024

Klebsiella pneumoniae strains that are resistant to multiple drugs (KPMDRs), which often acquired in hospital settings and lead healthcare-associated infections, pose a serious public health threat, as does hypervirulent K. (hvKp), can also cause infections otherwise healthy individuals. The widespread unnecessary use of antibiotics seen during the recent COVID-19 pandemic has exacerbated challenges posed by antibiotic resistance clinical settings. There is growing concern (hvKp) may acquire genes confer antimicrobial resistance, thus combining an MDR profile with their increased ability spread body sites, causing difficult-to-treat infections. This study aimed compare virulence profiles KPC-3-producing isolates collected over four years (2020–2023). A genome-based surveillance all CRE-K. was used identify genetic differences characterize profiles. Our results provide picture evolution contribute avoiding possible characteristics multi-drug virulence, thought be one main global health, within our hospital.

Language: Английский

Citations

3
Minimum inhibitory concentrations of aztreonam–avibactam, ceftazidime–avibactam and meropenem in clinical isolates of Klebsiella pneumoniae harboring carbapenemase genes DOI
V. A. Ageevets, O. S. Sulian,

Alisa Avdeeva

et al.

The Journal of Antibiotics, Journal Year: 2024, Volume and Issue: 77(10), P. 706 - 710

Published: July 1, 2024

Language: Английский

Citations

3
Comparison of Carbapenemases and Extended-Spectrum β-Lactamases and Resistance Phenotypes in Hospital- and Community-Acquired Isolates of Klebsiella pneumoniae from Croatia DOI Creative Commons
Haris Car,

Mirela Dobrić,

M Pospísil

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(11), P. 2224 - 2224

Published: Nov. 2, 2024

harbors various antibiotic resistance determinants like extended-spectrum and plasmid-mediated AmpC β-lactamases carbapenemases. In the last three years, in period of intense population aging, migrations climate changes Europe Croatia as well, we observed patters carbapenem-resistant

Language: Английский

Citations

3
High frequency of acquired virulence factors in carbapenemase-producing Klebsiella pneumoniae isolates from a large German university hospital, 2013–2021 DOI Creative Commons
Janko Sattler, Christoph M. Ernst,

Janine Zweigner

et al.

Antimicrobial Agents and Chemotherapy, Journal Year: 2024, Volume and Issue: 68(11)

Published: Oct. 4, 2024

ABSTRACT Carbapenemase-producing Klebsiella pneumoniae (CP-Kp) isolates are a public health concern as they can cause severe hospital-acquired infections that difficult to treat. It has recently been shown CP-Kp take up virulence factors from hypervirulent K. lineages. In this study, 109 clinical the University Hospital Cologne were examined for presence of acquired using whole-genome sequencing and phenotypic tests, results linked data. The factor iuc was present in 18/109 isolates. Other factors, such ybt , cbt iro rmpA/rmpA2 peg-344 hypervirulence-associated capsule types detected various combinations among these -positive produced OXA-232 ( n = 7), OXA-48 6), OXA-48+NDM 3), NDM, KPC (each 1), 7/18 resistant ceftazidime-avibactam, colistin, and/or cefiderocol. Four carried hybrid plasmids harbored alongside carbapenemase genes bla NDM-1/5 or . 15/18 patients, isolated clinically manifest infection site. Among these, four patients had osteomyelitis, died pneumonia with OXA-232-producing ST231 isolates, three them part an outbreak. conclusion, frequently carbapenemase-producing Germany, warranting continuous monitoring caused by strains.

Language: Английский

Citations

2
Phenotypic and Molecular Detection of Carbapenemase-Producing Klebsiella pneumoniae Isolated from Patients Admitted to Teaching Hospitals in Shiraz, Iran DOI Open Access
Naser Samadi, Mehdi Kalani, Taher Azimi

et al.

Jundishapur Journal of Microbiology, Journal Year: 2024, Volume and Issue: 16(12)

Published: Feb. 6, 2024

Background: Carbapenem-resistant Klebsiella pneumoniae (Cr-KPN) poses a significant global public health challenge. Objectives: This study aimed to investigate the prevalence and expression levels of carbapenemase-encoding genes in Cr-KPN isolated from patients admitted teaching hospitals Shiraz, Iran. Methods: A total 671 distinct clinical samples were collected two Shiraz. Initial identification final confirmation K. isolates carried out using conventional biochemical tests PCR assays, respectively. The detection carbapenemase-producing pneumoniae, both phenotypically genotypically, was performed through modified carbapenem inactivation methods (mCIM) multiplex assays. Real-time utilized assess genes. Results: overall frequency strains 14.9% (n = 100/671). mCIM indicated that 26% exhibited carbapenemase production. Furthermore, 24% 17% demonstrated resistance imipenem meropenem, blaIMI/IMP gene detected 91% isolates. Among imipenem-resistant isolates, 62.5% tested positive for blaOXA-48 gene. Additionally, 29.4%, 76.5%, 11.8% meropenem-resistant blaKPC, blaOXA-48, blaNDM genes, analysis revealed increased blaKPC (1.66-fold), (7.30-fold), (4.22-fold), blaIMI/NMC (2.39-fold) resistant when exposed imipenem. Conclusions: These findings underscore significance establishing active surveillance networks monitor track dissemination which presents threat.

Language: Английский

Citations

1
Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of Klebsiella pneumoniae DOI Open Access
Gloria Luz Paniagua‐Contreras,

Areli Bautista-Cerón,

Rosario Morales-Espinosa

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(19), P. 14754 - 14754

Published: Sept. 29, 2023

The emergence of hyper-virulent and multidrug-resistant (MDR) strains Klebsiella pneumoniae isolated from patients with hospital- community-acquired infections is a serious health problem that increases mortality. molecular analysis virulome expression related to antimicrobial-resistant genotype infection type in K. has been poorly studied. In this study, we analyzed the overall virulence associated antimicrobial resistance pulse field gel electrophoresis (PFGE) (PFtype) pneumoniae. We studied 25 who developed bacteremia pneumonia during their hospital stay 125 outpatients acquired infections. Susceptibility 12 antimicrobials was determined by Kirby–Bauer. identification antibiotic-resistance genes performed using polymerase chain reaction (PCR). To promote pneumoniae, an vitro model used human epithelial cell lines A549 A431. Bacterial RNA extracted QIAcube robotic workstation, reverse transcription cDNA Reverse Transcription QuantiTect kit (Qiagen). determination real-time PCR. addition, 57.3% (n = 86) were (MDR), mainly beta-lactam antibiotics (CB, AM, CFX, CF), aminoglycosides (GE), quinolones (CPF NOF), nitrofurantoin (NF), sulfamethoxazole/trimethoprim (SXT). most frequently expressed among adhesion-type, ycfm (80%), mrkD (51.3%), fimH (30.7%); iron uptake, irp2 (84%), fyuA (68.7%), entB (64.7%), irp1 (56.7%); protectins, rpmA (26%), which genes, blaTEM (96%), blaSHV (64%), blaCITM (52.6%), blaCTXM-1 (44.7%), tetA (74%), sul1 (57.3%), aac(3)-IV (40.7%), aadA1 (36%). results showed existence different patterns PFtypes cause These findings are important may contribute improving medical treatment strategies against caused

Language: Английский

Citations

3
Clinical Characteristics and Molecular Epidemiology of ST23 Klebsiella pneumoniae in China DOI Creative Commons
Y Q Liu,

Zijuan Jian,

Zhiqian Wang

et al.

Infection and Drug Resistance, Journal Year: 2023, Volume and Issue: Volume 16, P. 7597 - 7611

Published: Dec. 1, 2023

In clinical settings, CG23 Klebsiella pneumoniae (Kp) is the most virulent clonal group of Kp. Continuous fusions hypervirulent (Hv) and highly resistant strains have been reported; however, few studies analysed molecular epidemiology characteristics strains, especially MDR-sequence type ST23 strains. this study, we investigated Kp infections in a large teaching hospital third class China.ST23 isolates were screened using whole-genome sequencing data from single centre. We compared isolated community-acquired (CAI) acquired infection (HAI). addition, MDR poor-prognosis investigated. genetic further evolutionary relationship based on single-nucleotide polymorphism phylogenetic trees.We detected 184 between 2013 July 2018. There no significant differences isolation rates pulmonary, bloodstream, urinary tract, cutaneous soft tissue community hospitals, except for abscess infections. primarily cause pulmonary abscesses; with poor prognosis are typically bloodstream Fourteen producing extended-spectrum or C beta-lactamases, resulting resistance to third-generation cephalosporins. 3.8% clb locus was absent. The tree revealed that divided into three clades, data, it inferred transmission events occurred, mainly ICU causing lung infection.This study demonstrates virulence drug-resistance fusion occur gradually, clones facilitate widespread dissemination CAI HAI, particularly pulmonary. Monitoring genomics developing antivirulence strategies essential.

Language: Английский

Citations

3
Screening of Klebsiella pneumoniae Isolates for Carbapenemase and Hypervirulence-Associated Genes by Combining the Eazyplex® Superbug CRE and hvKp Assays DOI Creative Commons

Jürgen Rödel,

Yvonne Pfeifer, Martin A. Fischer

et al.

Antibiotics, Journal Year: 2023, Volume and Issue: 12(6), P. 959 - 959

Published: May 25, 2023

The acquisition of hypervirulence-associated genes by carbapenemase-producing Klebsiella pneumoniae is being increasingly observed, and easy-to-use diagnostic tests are needed for the surveillance hypervirulent K. (hvKp). In this pilot study, 87 isolates from invasive infections collected in 2022 2023 were analysed using LAMP-based eazyplex® Superbug CRE hvKp assays simultaneous identification carbapenemases virulence (rmpA/A2, iuC, iroC, ybt, clb). Nine showed a Kleborate score 4 or 5 (10.3%). time results ranged 6.5 to 13 min, total turnaround time, including sample preparation, was less than 30 min. Five isolates, three which produced New Delhi metallo-beta lactamase (NDM), subjected whole-genome sequencing (WGS) analysis further characterisation. test beta-lactamase confirmed. hvKp, currently only available as Research Use Only assay, may be useful tool rapid without significant additional workload when combined with assay detection carbapenemases.

Language: Английский

Citations

2