Phytochemical Constituents and Potential of Different Extracts from Eleutherine bulbosa on Skin Infectious Treatment : Anti-fungal, Anti-bacterial, Anti-inflammatory and Cytotoxic Activities DOI Open Access
Wannisa Raksamat, Ingorn Kimkong

Chiang Mai Journal of Science, Journal Year: 2023, Volume and Issue: 51(1), P. 1 - 13

Published: Dec. 25, 2023

Eleutherine bulbosa is a plant commonly employed in traditional medicine across various tropical regions, including Thailand. The aim of this study was to identify the phytochemical constituents E. bulbosa, evaluate inhibitory effects on skin fungal and bacterial pathogens, examine anti-inflammatory cytotoxic properties extracts obtained from bulbs through n-Hexane, EtOAc, EtOH extraction methods. Agar diffusion, MIC, MBC assays were determine fungistatic, bacteriostatic, bactericidal activities. EtOAc extract demonstrated highest inhibition against strains when compared other solvents. results revealed that exhibited potent anti-fungal effect T. rubrum (the zones = 35.00 ± 0.00 mm) comparable Ketoconazole 34.33 1.16 mm). antibacterial activity S. pyogenes observed, with zone diameter 23.00 1.00 mm, while lowest MIC values recorded at 19.53 78.12 μg/ml, respectively. Additionally, we significant extracted n-Hexane NO production LPS-stimulated RAW 264.7 cells solvent extracts. This occurred without affecting cell viability concentration range 6.25 25 μg/ml. Moreover, less affected fibroblasts suggests may be useful preventing inflammatory diseases mediated by excessive low toxicity normal cells. Phytochemical screening indicated presence compounds biological activities alkaloids, flavonoids, phenols, tannins all different These findings suggest bulb possess beneficial hold potential for further development as new natural source multifunctional therapeutic agents, which are safe effective broad spectrum infectious microorganisms also potentially reduce inflammation associated an infection caused microorganisms.

Language: Английский

Gram-negative endolysins: overcoming the outer membrane obstacle DOI Creative Commons
Hazel M. Sisson, Simon A. Jackson, Robert D. Fagerlund

et al.

Current Opinion in Microbiology, Journal Year: 2024, Volume and Issue: 78, P. 102433 - 102433

Published: Feb. 12, 2024

Our ability to control the growth of Gram-negative bacterial pathogens is challenged by rising antimicrobial resistance and requires new approaches. Endolysins are phage-derived enzymes that degrade peptidoglycan therefore offer potential as agents. However, outer membrane (OM) bacteria impedes access externally applied endolysins peptidoglycan. This review highlights recent advances in discovery characterization natural can breach OM, well chemical engineering approaches increase efficacy against pathogens.

Language: Английский

Citations

17
Engineering strategies and challenges of endolysin as an antibacterial agent against Gram‐negative bacteria DOI Creative Commons

Tianyu Zheng,

Can Zhang

Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(4)

Published: April 1, 2024

Bacteriophage endolysin is a novel antibacterial agent that has attracted much attention in the prevention and control of drug-resistant bacteria due to its unique mechanism hydrolysing peptidoglycans. Although exhibits excellent bactericidal effects on Gram-positive bacteria, presence outer membrane Gram-negative makes it difficult lyse them extracellularly, thus limiting their application field. To enhance extracellular activity facilitate crossing through researchers have adopted physical, chemical, molecular methods. This review summarizes characterization targeting strategies for modification, challenges future engineering against clinical applications, promote bacteria.

Language: Английский

Citations

11
Discovery of Antimicrobial Lysins from the “Dark Matter” of Uncharacterized Phages Using Artificial Intelligence DOI Creative Commons
Yue Zhang, Runze Li, Geng Zou

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(32)

Published: June 20, 2024

Abstract The rapid rise of antibiotic resistance and slow discovery new antibiotics have threatened global health. While novel phage lysins emerged as potential antibacterial agents, experimental screening methods for pose significant challenges due to the enormous workload. Here, first unified software package, namely DeepLysin, is developed employ artificial intelligence mining vast genome reservoirs (“dark matter”) lysins. Putative are computationally screened from uncharacterized Staphylococcus aureus phages 17 randomly selected validation. Seven candidates exhibit excellent in vitro activity, with LLysSA9 exceeding that best‐in‐class alternative. efficacy further demonstrated mouse bloodstream wound infection models. Therefore, this study demonstrates integrating computational approaches expedite proteins combating increasing antimicrobial resistance.

Language: Английский

Citations

10
Exploration of the feasibility of clinical application of phage treatment for multidrug-resistant Serratia marcescens -induced pulmonary infection DOI Creative Commons
Xiangke Duan, Wenfeng Liu,

Yanyu Xiao

et al.

Emerging Microbes & Infections, Journal Year: 2025, Volume and Issue: 14(1)

Published: Jan. 7, 2025

Serratia marcescens (S. marcescens) commonly induces refractory infection due to its multidrug-resistant nature. To date, there have been no reports on the application of phage treatment for S. infection. This study was conducted explore feasibility in treating by collaborating with a 59-year-old male patient pulmonary marcescens. Our experiments included three domains: i) selection appropriate phage, ii) verification efficacy and safety selected iii) confirmation phage-bacteria interactions. results showed that Spe5P4 is Treatment good efficacy, manifested as amelioration symptoms, hydrothorax examinations, chest computed tomography findings. Phage did not worsen hepatic renal function, immunity-related indices, or indices routine blood examination. It induce deteriorate drug resistance involved antibiotics. Importantly, adverse events were reported during follow-up periods. Thus, satisfactory safety. Finally, we found increase bacterial load, cytotoxicity, virulence, marcescens, indicating interactions between which are useful future against work provides evidence working basis further infections. We also provided methodological investigating clinical infections future.

Language: Английский

Citations

1
Phage lysins for intestinal microbiome modulation: current challenges and enabling techniques DOI Creative Commons

Iris Pottie,

Roberto Vázquez, Tom Van de Wiele

et al.

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: Aug. 6, 2024

The importance of the microbiota in intestinal tract for human health has been increasingly recognized. In this perspective, microbiome modulation, a targeted alteration microbial composition, gained interest. Phage lysins, peptidoglycan-degrading enzymes encoded by bacteriophages, are promising new class antibiotics currently under clinical development treating bacterial infections. Due to their high specificity, lysins considered microbiome-friendly. This review explores opportunities and challenges using as modulators. First, specificity endolysins, which can be further modulated protein engineering or delivery methods, is discussed. Next, obstacles possible solutions assess microbiome-friendliness considered. Finally, lysin discussed, including methods such particle-based probiotic vehicles. Mapping hurdles developing modulators identifying ways overcome these help development. way, application innovative antimicrobial agents expanded, thereby taking full advantage characteristics.

Language: Английский

Citations

5
Prevalence of Zoonotic Diseases in the Northeastern region, One Health perspective DOI Creative Commons

Puspakhi Borah,

Pankaj Das,

Ramashankar Bordoloi

et al.

Animals and zoonoses., Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Understanding the pathophysiology of Pseudomonas aeruginosa colonization as a guide for future treatment for chronic leg ulcers DOI Creative Commons
Gabriela Gonzalez Matheus, Michelle N. Chamoun,

Kiarash Khosrotehrani

et al.

Burns & Trauma, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 1, 2025

Chronic leg wounds represent a major burden of disease worldwide, costing health care systems billions dollars each year. Aside from the financial implications, they also impose significant physical and psychosocial on patient, their relatives and/or carers, community. Whilst measures such as maintenance wound hygiene, debridement, dressings compression are current standard care, complete healing is not always achievable ulcer recurrence common. Thus, there still gap to breach in terms understanding intricate pathophysiology chronic role this plays treatment management. Pseudomonas aeruginosa has been linked poor healing, with pathogen being frequently isolated ulcers. Characterized by its multi-drug resistance, targeting P. requires development novel therapeutic options. aim literature review describe ulcers discuss strategies. Here, we key molecular mechanisms driving observed clinical effect strategies common bacteria, establishing new approaches that could benefit patients hard heal wounds.

Language: Английский

Citations

0
Efficacy of Phage Application in Modulating Raw Milk Microbiota: Targeting Escherichia coli, Pseudomonas fluorescens, and Lactiplantibacillus plantarum DOI
Esra Ekiz, Kubra Guven, Emine Kübra Tayyarcan

et al.

Food Control, Journal Year: 2025, Volume and Issue: unknown, P. 111166 - 111166

Published: Jan. 1, 2025

Language: Английский

Citations

0
Therapeutic efficacy of LysGH15 against necrotising pneumonia caused by Staphylococcus aureus in a rabbit model DOI Creative Commons
Bowei Zhang,

Liran Song,

Zhanhua Wang

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 10, 2025

Staphylococcus aureus (S. aureus) is one of the most important zoonotic pathogens and can be transmitted to humans through meat diet routes, causing necrotising pneumonia. This study investigated therapeutic effect bacteriophage lysin LysGH15 on pneumonia in rabbit model caused by S. aureus. In vitro experiments, 50 μg/mL not only significantly reduced viable count (approximately 3.24 × 106 CFU/g) chicken stored at 4°C for 48 h but also effectively thawed 30°C, with reductions approximately 1.42 CFU/g 2.78 CFU/g, respectively. vivo a single intranasal administration 300 μg/rabbit increased survival rate rabbits 60%. At 72 postinfection, number bacteria lung tissues treated was 7 104 which lower than that PBS (7.76 or linezolid (6.38 105 CFU/g). addition, treatment alleviated tissue damage infected levels Panton-Valentine leukocidin (PVL), alpha-toxin (Hla), cytokines IFN-γ, TNF-α, IL-8 their tissues, similar those linezolid. These results suggest has potential used as novel antimicrobial agent

Language: Английский

Citations

0
Capsular Polysaccharide as a Potential Target in Hypervirulent and Drug-Resistant Klebsiella pneumoniae Treatment DOI Creative Commons
Yiying Ke, Zhangrui Zeng, Jinbo Liu

et al.

Infection and Drug Resistance, Journal Year: 2025, Volume and Issue: Volume 18, P. 1253 - 1262

Published: March 1, 2025

Klebsiella pneumoniae is a common cause of antimicrobial-resistant opportunistic infections in hospitalized patients. Hypervirulent (hvKP) acquiring exogenous resistance-encoding and hypervirulence-encoding genetic elements tend to develop both high virulence resistance. The management hvKP has also been made more difficult. Capsular polysaccharide (CPS) the most important virulent factor hvKP. degree heterogeneity CPS its hindering function adds difficulties finding general therapeutic approach. Thus, it imperative effective ways target CPS. development CPS-targeting phage treatment spurred scientific interest. relative vaccines show great potential as alternatives currently ineffective antibiotics. To find out new ideas for clinical practice, we reviewed molecular pathogenesis K. pneumoniae, discussed biological functions regulatory factors We studied roles virulence, drug resistance, preliminarily investigated viability prevention therapy infection.

Language: Английский

Citations

0