Genome-Centric Metatranscriptomics Analysis Reveals the Role of Hydrochar in Anaerobic Digestion of Waste Activated Sludge

Environmental Science & Technology, Journal Year: 2021, Volume and Issue: 55(12), P. 8351 - 8361

Published: May 24, 2021

Anaerobic digestion (AD) of waste activated sludge (WAS) has been widely used, while it poses problems including low methane yield and production rate. Hydrochar is produced by hydrothermal liquefaction biomass; however, little known about the role hydrochar in promoting AD WAS. The present study showed that increased rate 30.8% 31.4% pretreated dewatered enhancing acidification methanogenesis processes. Genomic-centric metatranscriptomics were used to identify metabolic activities transcriptomic response individual metagenome-assembled genomes enriched hydrochar. Although Methanosarcina sp. FDU0106 had shown unable H2, complete pathway for reduction CO2 methane. Syntrophomonas FDU0164 expressed genes extracellular electron transfer via electrically pili, suggesting exchanging electrons direct interspecies transfer. expression pili was decreased, indicating could replace its roles. Additionally, Firmicutes FDU0048, Proteiniphilum FDU0082, Aminobacterium mobile FDU0089 related degradation organics, which be enhanced yield.

Language: Английский

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Principles and advancements in improving anaerobic digestion of organic waste via direct interspecies electron transfer

Renewable and Sustainable Energy Reviews, Journal Year: 2021, Volume and Issue: 148, P. 111367 - 111367

Published: June 19, 2021

Language: Английский

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Enhancement of methane production from waste activated sludge using hybrid microbial electrolysis cells-anaerobic digestion (MEC-AD) process – A review

Bioresource Technology, Journal Year: 2021, Volume and Issue: 346, P. 126641 - 126641

Published: Dec. 29, 2021

Language: Английский

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Quorum Sensing Enhances Direct Interspecies Electron Transfer in Anaerobic Methane Production

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(6), P. 2891 - 2901

Published: Feb. 3, 2024

Direct interspecies electron transfer (DIET) provides an innovative way to achieve efficient methanogenesis, and this study proposes a new approach upregulate the DIET pathway by enhancing quorum sensing (QS). Based on long-term reactor performance, QS enhancement achieved more vigorous methanogenesis with 98.7% COD removal efficiency. In control system, failure occurred at accumulated acetate of 7420 mg COD/L lowered pH 6.04, much lower 41.9% was observed. The significant in QS-enhancing system supported higher expression conductive pili c-Cyts cytochrome secretion-related genes, resulting 12.7- 10.3-fold improvements. Moreover, also improved energy production capability, increase F-type V/A-type ATPase 6.3- 4.2-fold, effect probably provided for nanowires secretion. From perspective community structure, increased abundance Methanosaeta Geobacter from 54.3 17.6% 63.0 33.8%, respectively. Furthermore, genes involved carbon dioxide reduction alcohol dehydrogenation 0.6- 7.1-fold, Taken together, indicates positive effects chemicals stimulate advances understanding environments such as anaerobic digesters sediments.

Language: Английский

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Zero-valent iron enhanced methane production of anaerobic digestion by reinforcing microbial electron bifurcation coupled with direct inter-species electron transfer

Water Research, Journal Year: 2024, Volume and Issue: 255, P. 121428 - 121428

Published: March 5, 2024

Language: Английский

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