Promiscuous NAD-dependent dehydrogenases enable efficient bacterial growth on the PET monomer ethylene glycol DOI
Minrui Ren,

Danni Li,

Holly Addison

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 28, 2024

Abstract Ethylene glycol is widely used as antifreeze agent and monomer of the ubiquitous plastic PET (polyethylene terephthalate). Its global production amounts to more than 50 million tons per year, it constitutes an environmental pollutant increasing concern. Although generally accepted that bacteria oxidize ethylene use growth substrate, enzymes involved in this process are not well understood. Here we show soil bacterium Paracoccus denitrificans able assimilate efficiently via NAD-dependent alcohol aldehyde dehydrogenases. Using comparative proteome analysis, identify a previously unknown gene cluster strongly expressed presence glycol. We report kinetic parameters cryo-EM structures EtgB EtgA, key encoded by etg cluster. These novel biocatalysts pave way for efficient biotechnological conversion furthermore transcriptional activator EtgR controls expression Directed evolution P. on results faster growing strains, which enabled increased activities EtgA. Bioinformatic analysis reveals variants thereof distributed among Proteobacteria, suggesting underappreciated role dehydrogenases microbial assimilation.

Language: Английский

Recent insights into the biosynthesis and biological activities of the peptide-derived redox cofactor mycofactocin DOI Creative Commons
Mark Ellerhorst, Vadim Nikitushkin, Walid K. Al‐Jammal

et al.

Natural Product Reports, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Mycofactocin is a peptide-derived redox cofactor, commonly present in mycobacteria. This review covers its structural diversity, synthesis, biosynthesis and biochemical functions. Proteins interacting with the molecule are presented as well.

Language: Английский

Citations

0
Promiscuous NAD-dependent dehydrogenases enable efficient bacterial growth on the PET monomer ethylene glycol DOI
Minrui Ren,

Danni Li,

Holly Addison

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 28, 2024

Abstract Ethylene glycol is widely used as antifreeze agent and monomer of the ubiquitous plastic PET (polyethylene terephthalate). Its global production amounts to more than 50 million tons per year, it constitutes an environmental pollutant increasing concern. Although generally accepted that bacteria oxidize ethylene use growth substrate, enzymes involved in this process are not well understood. Here we show soil bacterium Paracoccus denitrificans able assimilate efficiently via NAD-dependent alcohol aldehyde dehydrogenases. Using comparative proteome analysis, identify a previously unknown gene cluster strongly expressed presence glycol. We report kinetic parameters cryo-EM structures EtgB EtgA, key encoded by etg cluster. These novel biocatalysts pave way for efficient biotechnological conversion furthermore transcriptional activator EtgR controls expression Directed evolution P. on results faster growing strains, which enabled increased activities EtgA. Bioinformatic analysis reveals variants thereof distributed among Proteobacteria, suggesting underappreciated role dehydrogenases microbial assimilation.

Language: Английский

Citations

1