Evaluating the coral microbiome during cryopreservation DOI Creative Commons

Tori Jefferson,

E. Michael Henley, Patrick M. Erwin

et al.

Cryobiology, Journal Year: 2024, Volume and Issue: 117, P. 104960 - 104960

Published: Sept. 17, 2024

Coral reefs are threatened by various local and global stressors, including elevated ocean temperatures due to anthropogenic climate change. cryopreservation could help secure the diversity of corals. Recently, isochoric vitrification was used demonstrate that coral fragments lived 24 hr post-thaw; however, in this study, they were stressed post-thaw. The microbial portion holobiont has been shown affect host fitness impact treatment on microbiomes is unknown. Therefore, we examined coral-associated bacterial communities pre- post-cryopreservation treatments, with a view towards informing potential future stress reduction strategies. We characterized microbiome Hawaiian finger coral, Porites compressa wild at seven steps during process. observed significant changes composition, including: 1) natural P. dominated Endozoicomonadaceae (76.5 % relative abundance) consistent between samples, independent collection location across Kāne'ohe Bay; 2) reduced <6.9 captivity, further <0.5 abundance after vitrification; 3) Vibrionaceae post-thaw (58.5-74.7 abundance). Thus, capture processes, implicated as possible causal agents dysbiosis loss putatively beneficial symbionts (Endozoicomonadaceae) overgrowth pathogens (Vibrionaceae). Offsetting these probiotic restoration treatments may alleviate improve husbandry.

Language: Английский

Divergent responses of the coral holobiont to deoxygenation and prior environmental stress DOI Creative Commons
Sara D. Swaminathan, Julie L. Meyer, Maggie D. Johnson

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 10

Published: Feb. 1, 2024

Ocean deoxygenation is intensifying globally due to human activities – and emerging as a grave threat coral reef ecosystems where it can cause bleaching mass mortality. However, one of many threats reefs, making essential understand how prior environmental stress may influence responses deoxygenation. To address this question, we examined the holobiont (i.e., host, Symbiodiniaceae, microbiome) in corals with different backgrounds. We outplanted Acropora cervicornis fragments known genotypes from an situ nursery two sites Florida Keys spanning inshore-offshore gradient. After four months, were transferred laboratory, tested differences survivorship, tissue loss, photosynthetic efficiency, Symbiodiniaceae cell density, microbiome composition after persistent exposure oxygen treatments ranging extreme (0.5 mg L -1 ) normoxia (6 ). found that, for short duration study (four days), entire was resistant dissolved (DO) concentrations low 2.0 , but that members decoupled at 0.5 . In most treatment, host showed decreased mortality, lower densities response, microbial taxa remained stable. Although did not major community shifts composition, population abundance some respond. Site history influenced endosymbiont, microbiome, more stressful inshore site showing greater susceptibility subsequent Our reveals respond differently deoxygenation, sensitivity resistance decrease tolerance

Language: Английский

Citations

4
Endozoicomonas dominance and Vibrionaceae stability underpin resilience in urban coral Madracis auretenra DOI Creative Commons
Jordan Steven Ruiz-Toquica, Andrés Franco-Herrera, Mónica Medina

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e19226 - e19226

Published: April 15, 2025

Coral resilience varies across species, with some exhibiting remarkable stability and adaptability, often mediated by their associated microbiomes. Given the species-specific nature of coral-microbiome interactions, investigating microbiomes urban-adapted corals provides critical insights into health, dynamics, functioning coral holobionts. In this study, we examined microbiome Madracis auretenra , a Caribbean from Santa Marta, Colombia, contrasting environmental conditions. Over two years, compared healthy stressed colonies distinct reef habitats—urban protected—using 16S rRNA gene sequencing (V4 region) to assess microbial diversity. Our findings revealed richness diversity were primarily influenced seasonal local factors rather than host-specific traits such as interaction algae, health status, or microhabitat. These variations not substantial enough disrupt overall community structure, which remained stable temporal spatial scales. Dominant taxa included Endozoicomonas along Vibrionaceae Rhodobacteraceae, form dense ecological networks. Notably, nutrient oxygen levels emerged key drivers fluctuations, yet populations exhibited exceptional stability. highlight presence well-structured resilient minimal variability, even in urban-influenced environments. We propose that dominance play pivotal role maintaining balance, ultimately contributing M. dynamic habitats.

Language: Английский

Citations

0
An assessment of hypoxia-induced coral death via mathematical modeling DOI
Parimita Roy, Sergei Petrovskii, Atul K. Jain

et al.

Nonlinear Dynamics, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Language: Английский

Citations

0
Microbiota Dysbiosis in Mytilus chilensis Is Induced by Hypoxia, Leading to Molecular and Functional Consequences DOI Creative Commons
Milton Montúfar-Romero, Diego Valenzuela‐Miranda, Valentina Valenzuela-Muñóz

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 825 - 825

Published: April 5, 2025

Bivalve microbiota play a vital role in host health, supporting nutrient processing, immunity, and disease resistance. However, the increasing hypoxia Chilean coastal waters, caused by climate change eutrophication, threatens to disrupt this microbial balance, potentially promoting pathogens impairing essential functions. Mytilus chilensis is vulnerable hypoxia-reoxygenation cycles, yet effects on its remain poorly understood. This study investigates impact of structure functional potential communities residing gills digestive glands M. chilensis. Employing full-length 16S rRNA gene sequencing, we explored hypoxia’s diversity capacity. Our results revealed significant alterations composition, with shift towards facultative anaerobes thriving low oxygen environments. Notably, there was decrease dominant bacterial taxa such as Rhodobacterales, while opportunistic Vibrio Aeromonas exhibited increased abundance. Functional analysis indicated decline critical functions associated metabolism immune support, jeopardizing health survival host. sheds light intricate interactions between host-associated environmental stressors, underlining importance managing face aquaculture practices.

Language: Английский

Citations

0
Evaluating the coral microbiome during cryopreservation DOI Creative Commons

Tori Jefferson,

E. Michael Henley, Patrick M. Erwin

et al.

Cryobiology, Journal Year: 2024, Volume and Issue: 117, P. 104960 - 104960

Published: Sept. 17, 2024

Coral reefs are threatened by various local and global stressors, including elevated ocean temperatures due to anthropogenic climate change. cryopreservation could help secure the diversity of corals. Recently, isochoric vitrification was used demonstrate that coral fragments lived 24 hr post-thaw; however, in this study, they were stressed post-thaw. The microbial portion holobiont has been shown affect host fitness impact treatment on microbiomes is unknown. Therefore, we examined coral-associated bacterial communities pre- post-cryopreservation treatments, with a view towards informing potential future stress reduction strategies. We characterized microbiome Hawaiian finger coral, Porites compressa wild at seven steps during process. observed significant changes composition, including: 1) natural P. dominated Endozoicomonadaceae (76.5 % relative abundance) consistent between samples, independent collection location across Kāne'ohe Bay; 2) reduced <6.9 captivity, further <0.5 abundance after vitrification; 3) Vibrionaceae post-thaw (58.5-74.7 abundance). Thus, capture processes, implicated as possible causal agents dysbiosis loss putatively beneficial symbionts (Endozoicomonadaceae) overgrowth pathogens (Vibrionaceae). Offsetting these probiotic restoration treatments may alleviate improve husbandry.

Language: Английский

Citations

0