Discrepant diversity patterns and function of bacterial and fungal communities on an earthquake-prone mountain gradient in Northwest Sichuan, China DOI Creative Commons

Tianzhi Huang,

Yingyan Wang, Xuemei Wang

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 22, 2023

Patterns of microbial diversity on elevational gradients have been extensively studied, but little is known about those patterns during the restoration earthquake-fractured alpine ecosystems. In this study, soil properties, enzyme activities, abundance and bacterial fungal communities at four positions along a 2.6-km gradient in Snow Treasure Summit National Nature Reserve, located Pingwu County, Southwest China. Although there were no significant changes chemical environment, significantly different elevations. The overall community presented an N-shaped pattern with increasing elevation, while decreased elevation. Changes associated phosphorus, plant litter, variations dominant taxa. Differences activities among elevations regulated by communities, catalase acid phosphatase mainly controlled Acidobacteria Planctomycetaceae bacteria, respectively (catalase: p < 0.001; phosphatase: 0.01), β-glucosidase, sucrase, urease fungi. β-glucosidase sucrase both positively correlated Herpotrichiellaceae, was Sebacinaceae (p 0.05). These findings contribute to conservation management mountain ecosystems face changing environmental conditions. Further research can delve into specific interactions between vegetation gain deeper insights intricate ecological dynamics within earthquake-prone

Language: Английский

Influence of bloom stage on the effectiveness of algicidal bacteria in controlling harmful cyanobacteria: A microcosm study DOI
Jesús Morón-López, Arnoldo Font-Nájera,

Mikołaj Kokociński

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 126261 - 126261

Published: April 1, 2025

Language: Английский

Citations

0
The synchronicity of bloom-forming cyanobacteria transcription patterns and hydrogen peroxide dynamics DOI Creative Commons
Taylor L. Hancock, Elizabeth K. Dahedl, Michael A. Kratz

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 348, P. 123812 - 123812

Published: March 23, 2024

Language: Английский

Citations

3
Uptake of Phytoplankton-Derived Carbon and Cobalamins by Novel Acidobacteria Genera in Microcystis Blooms Inferred from Metagenomic and Metatranscriptomic Evidence DOI
Derek J. Smith, Jenan J. Kharbush, Roland D. Kersten

et al.

Applied and Environmental Microbiology, Journal Year: 2022, Volume and Issue: 88(14)

Published: July 5, 2022

Interactions between bacteria and phytoplankton can influence primary production, community composition, algal bloom development. However, these interactions are poorly described for many consortia, particularly freshwater bloom-forming cyanobacteria. Here, we assessed the gene content expression of two uncultivated Acidobacteria from Lake Erie Microcystis blooms. These organisms were targeted because they previously identified as important catalase producers in blooms, suggesting that protect H2O2. Metatranscriptomics revealed both transcribed genes uptake organic compounds known cyanobacterial products exudates, including lactate, glycolate, amino acids, peptides, cobalamins. Expressed acid metabolism peptide transport degradation suggest use acids peptides by may regenerate nitrogen cyanobacteria other organisms. The genomes lacked biosynthesis cobalamins but expressed its remodeling. This indicates obtained externally, potentially Microcystis, which has a complete repertoire pseudocobalamin biosynthesis; them field samples; produced axenic culture. Both detected blooms worldwide. Together, data support hypotheses uncultured unidentified taxa exchange metabolites with during harmful available to phytoplankton. Thus, novel play role physiology IMPORTANCE heterotrophic competition successions taxa, thereby influencing ecosystem-wide processes such carbon cycling cyanobacterium forms freshwaters worldwide grows buoyant colonies harbor their phycospheres. Bacteria phycosphere surrounding likely ecology thus development impacts mechanisms interaction not fully understood. study explores members situ population genome resolution investigate cooccurrence

Language: Английский

Citations

16
Indole-3-acetic acid promotes growth in bloom-forming Microcystis via an antioxidant response DOI Creative Commons
Hunter R. Baylous, Matthew F. Gladfelter, Malia I. Gardner

et al.

Harmful Algae, Journal Year: 2024, Volume and Issue: 133, P. 102575 - 102575

Published: Jan. 21, 2024

Interactions between bacteria and phytoplankton in the phycosphere facilitate constrain biogeochemical cycling aquatic ecosystems. Indole-3-acetic acid (IAA) is a bacterially produced chemical signal that promotes growth of plants. Here, we explored impact IAA on bloom-forming cyanobacteria their associated bacteria. Exposure to its precursor, tryptophan, resulted strong response bloom freshwater cyanobacterium, Microcystis. Metatranscriptome analysis revealed induction an antioxidant Microcystis upon exposure IAA, potentially allowing populations increase photosynthetic rate overcome internally generated reactive oxygen. Our data reveal co-occurring within microbiome exhibit division labor for supportive functions, such as nutrient mineralization transport, vitamin synthesis, oxygen neutralization. These complex dynamics are example interactions microenvironment can have ecosystem-scale consequences.

Language: Английский

Citations

2
The harmful cyanobacterium Microcystis aeruginosa PCC7806 is more resistant to hydrogen peroxide at elevated CO2 DOI Creative Commons

Hongjie Qin,

Giovanni Sandrini,

Tim Piel

et al.

Harmful Algae, Journal Year: 2023, Volume and Issue: 128, P. 102482 - 102482

Published: Aug. 2, 2023

Rising atmospheric CO2 can intensify harmful cyanobacterial blooms in eutrophic lakes. Worldwide, these are an increasing environmental concern. Low concentrations of hydrogen peroxide (H2O2) have been proposed as a short-term but eco-friendly approach to selectively mitigate blooms. However, sensitivity cyanobacteria H2O2 vary depending on the available resources. To find out how respond under elevated CO2, Microcystis aeruginosa PCC 7806 was cultured chemostats with nutrient-replete medium C-limiting and C-replete conditions (150 ppm 1500 respectively). exposed high showed higher cell densities, biovolumes, microcystin contents, lower photosynthetic efficiency pH compared cultures grown low CO2. Subsamples were treated different (0-10 mg·L-1 H2O2) batch two light intensities (15 100 μmol photons m-2·s-1) response vitality monitored during 24 h. Results that more resistant at than carbon-limited conditions. Both CO2-adapted cells sensitive light. Microcystins (MCs) leaked 2-10 H2O2, while sum intra- extracellular MCs decreased. Although both lakes many factors, results imply it may become difficult suppress when continue rise.

Language: Английский

Citations

6
Expression of Microcystis Biosynthetic Gene Clusters in Natural Populations Suggests Temporally Dynamic Synthesis of Novel and Known Secondary Metabolites in Western Lake Erie DOI
Colleen E. Yancey,

Fengan Yu,

Ashootosh Tripathi

et al.

Applied and Environmental Microbiology, Journal Year: 2023, Volume and Issue: 89(5)

Published: April 18, 2023

Microcystis spp. produce diverse secondary metabolites within freshwater cyanobacterial harmful algal blooms (cyanoHABs) around the world. In addition to biosynthetic gene clusters (BGCs) encoding known compounds, genomes harbor numerous BGCs of unknown function, indicating a poorly understood chemical repertoire. While recent studies show that produces several in lab and field, little work has focused on analyzing abundance expression its broader suite during cyanoHAB events. Here, we use metagenomic metatranscriptomic approaches track relative their transcripts throughout 2014 western Lake Erie cyanoHAB. The results indicate presence transcriptionally active are predicted synthesize both novel metabolites. these shifted bloom, with transcript levels correlating temperature, nitrate, phosphorus concentrations co-occurring predatory competitive eukaryotic microorganisms, suggesting importance abiotic biotic controls regulating expression. This highlights need for understanding ecology potential risks human environmental health posed by produced but often unmonitored. It also indicates prospects identifying pharmaceutical-like molecules from cyanoHAB-derived BGCs. IMPORTANCEMicrocystis dominate worldwide pose significant threats water quality through production metabolites, many which toxic. toxicity biochemistry microcystins other compounds have been studied, remains understood, leaving gaps our impacts ecosystem health. We used community DNA RNA sequences diversity genes synthesis natural populations assess patterns transcription cyanoHABs. Our reveal encode toxic as well ones may cryptic compounds. research targeted metabolite Erie, vital source United States Canada.

Language: Английский

Citations

5
Variation in resource competition traits among Microcystis strains is affected by their microbiomes DOI Creative Commons
Dylan Baker, Casey M. Godwin,

Muhtamim Khanam

et al.

mLife, Journal Year: 2023, Volume and Issue: 2(4), P. 401 - 415

Published: Dec. 1, 2023

Abstract Freshwater harmful algal blooms are often dominated by Microcystis , a phylogenetically cohesive group of cyanobacteria marked extensive genetic and physiological diversity. We have previously shown that this diversity the presence microbiome heterotrophic bacteria influences competitive interactions with eukaryotic phytoplankton. In study, we sought to explain these observations characterizing Monod equation parameters for resource usage (maximum growth rate μ max half‐saturation value K s, quota) as function N P levels four strains (NIES‐843, PCC 9701, 7806 [WT], Δ mcyB ) in absence derived from isolated Lake Erie. Results indicated limited differences maximum rates but more pronounced values among strains. The largest impact was reducing minimal nitrogen concentration sustaining half saturation values, variable results depending on strain. also differed each other their quotas extent which affected them. Our data highlight importance altering ‐intrinsic traits, strain hierarchies, thus bloom dynamics. As quota, s commonly used models blooms, our suggest model improvement may be possible incorporating genotype dependencies resource‐use parameters.

Language: Английский

Citations

4
Microbial Community Response to Granular Peroxide-Based Algaecide Treatment of a Cyanobacterial Harmful Algal Bloom in Lake Okeechobee, Florida (USA) DOI Creative Commons
Forrest W. Lefler, Maximiliano Barbosa, David E. Berthold

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(5), P. 206 - 206

Published: April 26, 2024

Cyanobacterial harmful algal blooms (cyanoHABs) occur in fresh water globally. These can degrade quality and produce toxins, resulting ecological economic damages. Thus, short-term management methods (i.e., algaecides) are necessary to rapidly mitigate the negative impacts of cyanoHABs. In this study, we assess efficacy a hydrogen peroxide-based algaecide (PAK

Language: Английский

Citations

1
Selective increase of antibiotic-resistant denitrifiers drives N2O production in ciprofloxacin-contaminated soils DOI

Linfa Fang,

Yue Deng, Prakash Lakshmanan

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 479, P. 135673 - 135673

Published: Aug. 27, 2024

Language: Английский

Citations

1
A combination cyanobacterial treatment approach using hydrogen peroxide and L-lysine successfully improved the removal efficiency of toxic cyanobacteria DOI
Elizabeth K. Dahedl, Taylor L. Hancock, Michael A. Kratz

et al.

Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 373, P. 123503 - 123503

Published: Dec. 12, 2024

Language: Английский

Citations

1