Metabolic engineering of fast-growing Vibrio natriegens for efficient pyruvate production DOI Creative Commons
Fengli Wu, Shucai Wang, Yanfeng Peng

et al.

Microbial Cell Factories, Journal Year: 2023, Volume and Issue: 22(1)

Published: Sept. 4, 2023

Pyruvate is a widely used value-added chemical which also serves as hub of various metabolic pathways. The fastest-growing bacterium Vibrio natriegens promising chassis for synthetic biology applications with high substrate uptake rates. aim this study was to investigate if the rates V. enable pyruvate production at productivities.Two prophage gene clusters and several essential genes biosynthesis byproducts were first deleted. In order promote accumulation, key aceE encoding dehydrogenase complex E1 component down-regulated reduce carbon flux into tricarboxylic acid cycle. Afterwards, expression ppc phosphoenolpyruvate carboxylase fine-tuned balance cell growth synthesis. resulting strain PYR32 able produce 54.22 g/L from glucose within 16 h, yield 1.17 mol/mol an average productivity 3.39 g/L/h. addition, efficiently convert sucrose or gluconate titers.A novel engineered capable provide higher in This lays foundation its derivatives fast-growing natriegens.

Language: Английский

Allomelanin: A Biopolymer of Intrinsic Microporosity DOI Creative Commons
Naneki C. McCallum, Florencia A. Son, Tristan D. Clemons

et al.

Journal of the American Chemical Society, Journal Year: 2021, Volume and Issue: 143(10), P. 4005 - 4016

Published: March 5, 2021

Melanin is a ubiquitous natural pigment found in diverse array of organisms. Allomelanin class nitrogen-free melanin often fungi. Herein, we find artificial allomelanin analogues exhibit high intrinsic microporosity and describe an approach for further increasing tuning that porosity. Notably, the synthetic method involves oxidative polymerization 1,8-DHN water, negating need multiple complex templating steps avoiding expensive or chemical precursors. The well-defined morphologies these nanomaterials were elucidated by combination electron microscopy scattering methods, yielding to high-resolution 3D reconstruction based on small-angle X-ray (SAXS) results. Synthetic nanoparticles BET areas, up 860 m2/g, are capable ammonia capture 17.0 mmol/g at 1 bar. In addition, can adsorb nerve agent simulants solution as coating fabrics with breathability where they prevent breakthrough. We also confirmed naturally derived fungal gas efficiently despite lower porosity than analogues. Our inspires analysis yet be discovered biological materials this melanins may linked evolutionary advantages relevant organisms turn inspire design new surface area materials.

Language: Английский

Citations

52
Metabolic engineering of Vibrio natriegens DOI Creative Commons
Felix Thoma, Bastian Blombach

Essays in Biochemistry, Journal Year: 2021, Volume and Issue: 65(2), P. 381 - 392

Published: April 9, 2021

Vibrio natriegens is emerging as a promising host for biotechnology which basically due to the remarkable intrinsic properties such exceptionally high growth and substrate consumption rates. The facultatively anaerobic marine bacterium possesses versatile metabolism, able utilize variety of substrates carbon energy sources easy handle in lab. These features initiated rapid development genetic tools resulted extensive engineering production strains past years. Although recent examples illustrate potential V. biotechnology, comprehensive understanding metabolism its regulation still lacking but essential exploit full this bacterium. In review, we summarize current knowledge on physiological traits genomic organization, provide an overview available advances metabolic natriegens. Finally, discuss obstacles have be overcome order establish industrial host.

Language: Английский

Citations

50
The Marburg Collection: A Golden Gate DNA Assembly Framework for Synthetic Biology Applications in Vibrio natriegens DOI
Daniel Stukenberg,

Tobias Hensel,

Josef Hoff

et al.

ACS Synthetic Biology, Journal Year: 2021, Volume and Issue: 10(8), P. 1904 - 1919

Published: July 13, 2021

Vibrio natriegens is known as the world's fastest growing organism with a doubling time of less than 10 min. This incredible growth speed empowers V. chassis for synthetic and molecular biology, potentially replacing E. coli in many applications. While first genetic parts have been built tested natriegens, comprehensive toolkit containing well-characterized standardized did not exist. To close this gap, we created Marburg Collection—a highly flexible Golden Gate cloning toolbox optimized emerging 191 parts. The Collection overcomes paradigm plasmid construction—integrating inserts into backbone—by enabling de novo assembly plasmids from basic allows users to select replication origin resistance part independently, which advantageous when limited knowledge about behavior those target available. Additional design highlights are novel connector parts, facilitate modular circuit and, optionally, inversion individual transcription units reduce transcriptional crosstalk multigene constructs. quantitatively characterize contained developed reliable microplate reader measurement workflow reporter experiments overcame organism-specific challenges. We think its thoroughly characterized will provide valuable resource community.

Language: Английский

Citations

49
An extensive review of marine pigments: sources, biotechnological applications, and sustainability DOI Open Access
Luis Alfonso Muñoz-Miranda, Maricarmen Iñiguez‐Moreno

Aquatic Sciences, Journal Year: 2023, Volume and Issue: 85(3)

Published: April 18, 2023

Language: Английский

Citations

21
Selenomelanin: An Abiotic Selenium Analogue of Pheomelanin DOI
Wei Cao, Naneki C. McCallum, Qing Zhe Ni

et al.

Journal of the American Chemical Society, Journal Year: 2020, Volume and Issue: 142(29), P. 12802 - 12810

Published: July 8, 2020

Melanins are a family of heterogeneous biopolymers found ubiquitously across plant, animal, bacterial, and fungal kingdoms where they act variously as pigments radiation protection agents. There exist five multifunctional yet structurally biosynthetically incompletely understood varieties melanin: eumelanin, neuromelanin, pyomelanin, allomelanin, pheomelanin. Although eumelanin allomelanin have been the focus most studies to date, some research suggests that pheomelanin has better absorption coefficient for X-rays than eumelanin. We reasoned if selenium enriched melanin existed, it would be X-ray protector sulfur-containing because is proportional fourth power atomic number (Z). Notably, an essential micronutrient, with amino acid selenocysteine being genetically encoded in 25 natural human proteins. Therefore, we hypothesize selenomelanin exists nature, provides superior ionizing organisms compared known melanins. Here introduce this novel analogue through chemical biosynthetic routes using selenocystine feedstock. The resulting structural mimic effectively prevented neonatal epidermal keratinocytes (NHEK) from G2/M phase arrest under high-dose irradiation. Provocatively, beneficial role points sixth variety discovered melanin.

Language: Английский

Citations

46
High rate 2,3-butanediol production with Vibrio natriegens DOI Creative Commons
Anna Maria Erian,

Philipp Freitag,

Martin Gibisch

et al.

Bioresource Technology Reports, Journal Year: 2020, Volume and Issue: 10, P. 100408 - 100408

Published: Feb. 27, 2020

The aim of this study was to investigate if the high substrate turnover rates Vibrio natriegens enable 2,3-butanediol production at productivities. Introduction a heterologous pathway led yields comparable Escherichia coli using glucose, sucrose and sugar beet molasses. Microaerobic fed-batch cultivations maintained 0–1% dissolved oxygen yielded combined diol titer 49.9 g L−1 acetoin rate 3.9 h−1. Optimization supply in chemostat increased product yield 70% theoretical maximum volumetric specific 2 h−1 0.66 g−1 h−1, respectively. In conclusion, could for first time demonstrate V. as novel host with productivity, underlining its potential microbial chemical production.

Language: Английский

Citations

41
Vibrio natriegens as a pET-Compatible Expression Host Complementary to Escherichia coli DOI Creative Commons
Jiaqi Xu, Feng Dong,

Meixian Wu

et al.

Frontiers in Microbiology, Journal Year: 2021, Volume and Issue: 12

Published: Feb. 19, 2021

Efficient and novel recombinant protein expression systems can further reduce the production cost of enzymes. Vibrio natriegens is fastest growing free-living bacterium with a doubling time less than 10 min, which makes it highly attractive as host. Here, 196 pET plasmids different genes interest (GOIs) were electroporated into V. strain VnDX, carries an integrated T7 RNA polymerase cassette. As result, 65 75% tested GOIs obtained soluble in Escherichia coli , respectively, 20 showed better former. Furthermore, we have adapted consensus “what to try first” protocol for based on Terrific Broth medium. Six sampled encoding biocatalysts enzymes thus achieved 50–128% higher catalytic efficiency under optimized conditions. Our study demonstrated pET-compatible host spectrum expressed distinct from E. easy-to-use protocol, solving problem that some cannot be well .

Language: Английский

Citations

39
Bioprocess of Microbial Melanin Production and Isolation DOI Creative Commons
Kwon‐Young Choi

Frontiers in Bioengineering and Biotechnology, Journal Year: 2021, Volume and Issue: 9

Published: Nov. 16, 2021

Melanin is one of the most abundant pigments found in biosphere. Owing to its high biocompatibility and diverse biological activities, it has been widely applied as a functional biomaterial cosmetic, pharmaceutical, biopolymer, environmental fields. In this study, production melanin was comprehensively reviewed concerning bioconversion isolation processes. First, several melanogenic microbes, including fungi bacteria, were summarized. classified by host type analyzed titers g/L addition reaction conditions, pH temperature. The further interpreted using space-time yields chart, which showed two distinct classifications productivity, conditions pH-temperature-titer chart. Next, extraction process summarized crude pure preparation procedures, highlighted. Finally, recent applications briefly summarized, prospects for application development industrial suggested.

Language: Английский

Citations

39
Vibrio natriegens as a host for rapid biotechnology DOI
Jiaqi Xu, Sheng Yang, Lirong Yang

et al.

Trends in biotechnology, Journal Year: 2021, Volume and Issue: 40(4), P. 381 - 384

Published: Nov. 16, 2021

Language: Английский

Citations

37
Non-Sterilized Fermentation of 2,3-Butanediol with Seawater by Metabolic Engineered Fast-Growing Vibrio natriegens DOI Creative Commons
Wensi Meng, Yongjia Zhang, Liting Ma

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: July 12, 2022

Sustainable and environment-friendly microbial fermentation processes have been developed to produce numerous chemicals. However, the high energy input required for sterilization substantial fresh water consumption restrict economic feasibility of traditional processes. To address these problems, Vibrio natriegens, a promising chassis with low nutritional requirements, salt tolerance rapid growth rate can be selected as host chemical production. In this study, V. natriegens was metabolic engineered 2,3-butanediol (2,3-BD), an important platform chemical, through non-sterilized seawater-based minimal medium after expressing 2,3-BD synthesis cluster deleting two byproduct encoding genes. Under optimized fermentative conditions, 41.27 g/L produced productivity 3.44 g/L/h yield 0.39 g/g glucose by recombinant strain natriegensΔfrdAΔldhA-pETRABC. This study confirmed using seawater replace freshwater other valuable chemicals may also engineering emerging synthetic biology natriegens.

Language: Английский

Citations

28