Expert Review of Anti-infective Therapy, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 13, 2024
Invasive fungal infections (IFI) present a major medical challenge, with an estimated 6.5 million cases annually, resulting in 3.8 deaths. Pathogens such as Aspergillus spp. Candida Mucorales Cryptococcus and other fungi species contribute to these infections, posing risks immunocompromised individuals. Early accurate diagnosis is crucial for effective treatment better patient outcomes.
Language: Английский
Citations
5Clinical significance and antifungal susceptibility profile of 103 clinical isolates of Scedosporium species complex and Lomentospora prolificans obtained from NIH patients
Journal of Clinical Microbiology, Journal Year: 2025, Volume and Issue: unknown
Published: March 7, 2025
Reduced susceptibility to antifungals is common among members of genera Scedosporium and Lomentospora, with optimal treatments still not fully defined. In vitro antifungal results clinical data do comprehensively account for the advent new species identified by molecular phylogenetics. Using Clinical Laboratory Standards Institute (CLSI) methodology, we tested a total 103 isolates obtained from patients at NIH Center. The most frequent were apiospermum (63%) boydii (11%), followed Lomentospora prolificans (7%). novel olorofim showed lowest MICs against all spp. L. prolificans, micafungin. Among triazoles, voriconazole lower Amphotericin B posaconazole demonstrated species-specific inter-species variable activity. Itraconazole, isavuconazole, terbinafine had higher MIC values prolificans. retrospectively reviewed 90 isolates, which nine (28 isolates) active disease/infection received treatment that included or posaconazole. Five these (56%) died, while three (33%) chronic granulomatous disease cured following hematopoietic cell transplantation. 24 (62 isolates), presence fungus was considered airway colonization. conclusion, our support existence differences in patterns investigational may be promising therapy. Our suggest host status administration therapy effective each complex are important determinants outcomes.IMPORTANCEUnderstanding epidemiology spectrum infections caused integral improving outcomes, particularly severely ill immunocompromised patients. testing can provide an estimate activity fungal pathogens. study exist distribution between also highlight status, along therapy, plays crucial role determining outcomes.
Language: Английский
Citations
0Diagnosing invasive fungal infections in the laboratory today: It's all good news?
Revista Iberoamericana de Micología, Journal Year: 2025, Volume and Issue: unknown
Published: March 1, 2025
Language: Английский
Citations
0Clinical Significance and Therapeutic Challenges of Scedosporium spp. and Lomentospora prolificans Isolates in a Single-Center Cohort of Lung Transplant Recipients
Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(4), P. 291 - 291
Published: April 8, 2025
(1) Background: Emerging fungal infections associated with Scedosporium spp. and Lomentospora prolificans (S/L) are becoming more frequent very difficult to treat. Our objective was analyze the frequency management of S/L isolates in lung transplant (LTx) recipients, patient outcomes vitro antifungal sensitivity. (2) Methods: We included all patients isolation during post-transplant follow-up. Data were collected from electronic medical records. All samples cultivated on Sabouraud Chloramphenicol agar. Isolations submitted susceptibility tests. (3) Results: A total 11 (2%) 576 LTx recipients had at least one S/L. Classification for cases colonization (4; 36%) infection (7; 65%). Five pulmonary (71%) two disseminated (29%). S. apiospermum complex most frequently occurring disease while L. disease. Ten treated. The drugs used voriconazole (n = 8) terbinafine 6). Seven (70%) received than drug. mortality rate 50% 100% (4) Conclusions: high morbidity rates. New diagnostic therapeutic tools required reduce impact these infections.
Language: Английский
Citations
0Extracellular Vesicles from Scedosporium apiospermum Mycelial Cells: Implication for Fungal-Host Interplays
Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(4), P. 277 - 277
Published: April 9, 2024
The release of extracellular vesicles (EVs) has been implicated as an alternative transport mechanism for the passage macromolecules through fungal cell wall, a phenomenon widely reported in yeasts but poorly explored mycelial cells. In present work, we have purified and characterized EVs released by mycelia emerging, opportunistic, widespread multidrug-resistant filamentous fungus Scedosporium apiospermum. Transmission electron microscopy images light scattering measurements revealed EVs, which were observed individually or grouped with heterogeneous morphology, size density. mean diameter evaluated technique, was 179.7 nm. Overall, structural stability S. apiospermum preserved during incubation under various storage conditions. lipid, carbohydrate protein contents quantified, EVs’ profile evidenced SDS-PAGE, revealing proteins molecular masses ranging from 20 to 118 kDa. Through immunoblotting, ELISA immunocytochemistry assays, antigenic molecules using polyclonal serum (called anti-secreted molecules) rabbit inoculated conditioned cell-free supernatant obtained By Western blotting, several identified. assay confirmed that exhibited positive reaction up dilution 1:3200. Despite transporting immunogenic molecules, slightly induced vitro cytotoxicity effect after 48 h contact either macrophages lung epithelial Interestingly, pretreatment both mammalian cells significantly increased association index conidia. Furthermore, highly toxic Galleria mellonella, leading larval death typically dose- time-dependent manner. Collectively, results represent first report detecting form, highlighting possible implication pathogenesis.
Language: Английский
Citations
2New and emerging roles for inhalational and direct antifungal drug delivery approaches for treatment of invasive fungal infections
Expert Review of Anti-infective Therapy, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 24, 2024
The rising prevalence of difficult-to-treat, deep-seated invasive fungal diseases (IFD) has led to high mortality. Currently available antifungal treatments, administered predominantly orally or intravenously, may not sufficiently penetrate certain body sites, and/or are associated with systemic toxicity. Little is known about how position alternative administration approaches such as inhalational and direct drug delivery routes.
Language: Английский
Citations
1Clinical Microbiology Reviews : a 21st century vision
Clinical Microbiology Reviews, Journal Year: 2024, Volume and Issue: 37(3)
Published: June 20, 2024
Language: Английский
Citations
0The top 10 papers on the treatment of invasive fungal infections, 2018–2023
Therapeutic Advances in Infectious Disease, Journal Year: 2024, Volume and Issue: 11
Published: Jan. 1, 2024
Background: Invasive fungal infections are responsible for a large number of in hospitalized patients annually and high morbidity mortality. Familiarity with novel agents or strategies this area can be challenging. Objectives: To identify the top 10 manuscripts on treatment invasive from 2018 to 2023. Design: Modified Delphi consensus-building technique. Methods: A three-stage approach was used comprised (1) identifying relevant articles; (2) voting by panel experts establish consensus importance these (3) finalizing list articles small group. Members Southeastern Research Group Endeavor network served as content experts. Publications 2023 were evaluated if met following inclusion criteria: published between 2023, contained related infections, included an actionable intervention. Results: total 6518 potential publications assessed. After applying exclusion criteria, 82 reviewed. The selected experts, summarized manuscript include aspergillosis, candidiasis, cryptococcosis. Conclusion: This article highlights may serve key resource teaching training. Clinicians also employ reported interventions new opportunities optimize antifungal therapeutic within one’s institution.
Language: Английский
Citations
0Fungal ball due to a species of the Scedosporium apiospermum complex in a post-tuberculosis patient
Revista Iberoamericana de Micología, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 1, 2024
Language: Английский
Citations
0Non-Aspergillus Pulmonary Mold Infections
Published: Jan. 1, 2024
Language: Английский
Citations
0