Environmental Compatibility and Genome Flexibility of Klebsiella oxytoca Isolated from Eight Species of Aquatic Animals DOI Creative Commons

Shuo Sun,

Tingting Gu,

Yafei Ou

et al.

Diversity, Journal Year: 2024, Volume and Issue: 16(1), P. 30 - 30

Published: Jan. 2, 2024

Klebsiella oxytoca is an emerging pathogen that can cause life-threatening infectious diseases in humans. Recently, we firstly reported for the first time presence of K. edible aquatic animals. In this study, further investigated its bacterial environmental fitness and genome evolution signatures. The results revealed isolates (n = 8), originating from eight species animals, were capable growing under a broad spectrum conditions (pH 4.5–8.5, 0.5–6.5% NaCl), with different biofilm formation swimming mobility profiles. sequences determined (5.84–6.02 Mb, 55.07–56.06% GC content). Strikingly, numerous putative mobile genetic elements (MGEs), particularly genomic islands (GIs, n 105) prophages 24), found genomes, which provided bacterium specific adaptation traits, such as resistance, virulence, material metabolism. Interestingly, identified prophage-related clusters derived Burkholderia spp., Enterobacter Pseudomonas Haemophilus suggesting phage transmission across other four genera. Many strain-specific 10–447) genes present whereas CRISPR-Cas protein-encoding gene was absent, indicating likely active horizontal transfer (HGT) considerable variation evolution. Overall, study are to demonstrate compatibility flexibility animal origins.

Language: Английский

Core genome multilocus sequence typing (cgMLST) applicable to the monophyletic Klebsiella oxytoca species complex DOI Creative Commons
Johanna Dabernig‐Heinz, Gabriel E. Wagner,

Karola Prior

et al.

Journal of Clinical Microbiology, Journal Year: 2024, Volume and Issue: 62(6)

Published: May 23, 2024

The environmental bacterium

Language: Английский

Citations

4
Gut community structure as a risk factor for infection in Klebsiella pneumoniae -colonized patients DOI Creative Commons
Jay Vornhagen, Krishna Rao, Michael A. Bachman

et al.

mSystems, Journal Year: 2024, Volume and Issue: 9(8)

Published: Aug. 20, 2024

The primary risk factor for infection with members of the

Language: Английский

Citations

4
A rare case of sepsis caused by Klebsiella oxytoca and Aeromonas hydrophila DOI Creative Commons
Hua Long,

Ruirui Yang

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)

Published: Jan. 21, 2025

Language: Английский

Citations

0
Potential of lavender essential oil to inhibit tetracycline resistance and modulate gut microbiota in black soldier fly larvae DOI

Huawei Wei,

Jing Jiang, Yu Zhao

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 137345 - 137345

Published: Jan. 1, 2025

Language: Английский

Citations

0
The results of polymerase chain reaction and MALDI-TOF mass spectrometry versus phenotypic distinction between Klebsiella pneumoniae and Klebsiella oxytoca DOI Creative Commons
Agata Palusiak, Anna Maciejewska, Jolanta Łukasiewicz

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 27, 2025

Klebsiella pneumoniae and K. oxytoca are members of Enterobacteriaceae. They Gram-negative, non-motile rods that ubiquitous in the environment part human intestinal microbiota. These opportunistic pathogens may cause pneumonia urinary tract infections. species genetically biochemically similar; therefore, it is important to find reliable methods for their differentiation. This study presents results biochemical assays, PCR, MALDI-TOF mass spectrometry (MS) performed on 35 isolates obtained from urine patients central Poland. Among methods, indole test demonstrated highest discriminatory power, whereas determination growth at 10°C was least effective. For all strains identified as pneumoniae, a 108-bp amplicon detected, indicating presence rpoB gene genome. Only 12 produced product pehX gene. All tested were analyzed using Biotyper, which confirmed, with high-quality scores, identification based api 20E tests. Strain 0.011 Raoultella ornithinolytica. MS analysis proved be most method identifying potential phylogroup

Language: Английский

Citations

0
Antimicrobial resistance and biofilm formation of penile prosthesis isolates: insights from in-vitro analysis DOI
Bradley J. Roth,

Prajit Khooblall,

Navid Leelani

et al.

The Journal of Sexual Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Language: Английский

Citations

0
First report of blaVEB-3 and blaKPC-2 coexistence with a novel blaKPC-2 transposon in Klebsiella michiganensis DOI Creative Commons
Yu-Xia Zhong, Peibo Yuan, Li Dai

et al.

Infection Genetics and Evolution, Journal Year: 2025, Volume and Issue: unknown, P. 105740 - 105740

Published: March 1, 2025

Klebsiella michiganensis, an emerging opportunistic pathogen, poses public health risks due to its increasing multidrug resistance (MDR), especially carbapenems. A 46-year-old man with pulmonary fibrosis was hospitalized in Guangzhou, China, for worsening pneumonia. multidrug-resistant K. michiganensis strain (YK6) isolated from his sputum before treatment. The characterized using MALDI-TOF mass spectrometry, antimicrobial susceptibility testing (AST), and whole genome sequencing (WGS). Targeted therapy guided by AST successfully resolved the infection. YK6 exhibited carbapenems, β-lactam/β-lactamase inhibitors, cephalosporins, aminoglycosides, quinolones, except colistin tigecycline. Genomic analysis revealed a 41.9-kb MDR island intact I-E CRISPR-Cas system on chromosome, along two plasmids: IncFIA/IncFII plasmid pYK6-1 carrying blaKPC-2 IncC pYK6-2 harboring blaVEB-3. novel blaKPC-2-transposon identified, consisting of non-Tn4401 element (NTE)-like structure (Tn3-ISKpn27-blaKPC-2-ΔISKpn6-korC) flanked inversely oriented ISKpn19-tnpM-tnpR elements 31-bp inverted repeats never reported, configuration did not reported previously. Furthermore, blaVEB-3 genetic environment featured unique cassette: IS26-IS6100-blaVEB-3-tnp-ISAs1-qacEΔ1-sul1-ISCR1. An additional ISAs1 insertion between tnpF-like integrase qacEΔ1 distinguishes it similar blaVEB-3-harboring cassettes. region likely originated homologous recombination mediated IS26 Tn5403, which flank gene cassette. To our knowledge, this is first report concurrent transposon structure. These findings highlight urgent need enhanced surveillance prevent treatment failures.

Language: Английский

Citations

0
Unraveling the genomic epidemiology and plasmid-mediated carbapenem resistance of Klebsiella pasteurii DOI Creative Commons
Xinyue Li,

Zexuan Song,

Jinshuo Liu

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 17, 2025

This study isolated a Klebsiella pasteurii strain, K1134, from the sputum of an ICU patient, revealing its resistance to carbapenem antibiotics meropenem and imipenem. Whole-genome sequencing identified plasmid pK1134-KPC, which carries gene bla KPC-2. belonging IncFIIpCP020359 group, exhibits modular structure with KPC-2 embedded in 32.09 kb accessory region containing multiple genetic elements (AGEs). Comparative genomic analysis 48 K. isolates 12 countries showed high diversity, strains clustered into three clades. Notably, harbors extensive antimicrobial genes across diverse AGEs, classifying it as multidrug-resistant. Twelve KPC-carrying AGEs were sequences isolates, classified two groups: Tn7551 Tn6296-related elements. The clusters for enterotoxins tilimycin tilivalline, encompassing key regulators operons, present nearly all strains, incomplete exclusively observed clade 3 isolates. underscores global dissemination adaptability pasteurii, highlighting potential role reservoir emphasizing need robust surveillance mitigate public health impact.

Language: Английский

Citations

0
BCG therapy in bladder cancer and its tumor microenvironment interactions DOI Creative Commons

Jian Ni,

Lei Yu, Lijuan Ma

et al.

Clinical Microbiology Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

SUMMARYBacillus Calmette-Guérin (BCG) has been the standard treatment for non-muscle-invasive bladder cancer over 30 years. Despite its proven efficacy, challenges persist, including unclear mechanisms of action, resistance in 30%-50% patients, and significant side effects. This review presents an updated balanced discussion antitumor BCG, focusing on direct effects interactions with various cell types within tumor microenvironment. Notably, recent research between BCG microbiome is also incorporated. We further summarize analyze latest preclinical clinical studies regarding both intrinsic adaptive to cancer. Based current understanding BCG's therapeutic principles mechanisms, we systematically explore strategies improve BCG-based immunotherapy. These include development recombinant combination therapy different drugs, optimization regimens management, exploration new approaches by targeting changes microbiota metabolites. measures aim effectively address cancer, reduce toxicity, ultimately enhance anti-tumor efficacy. Bacterial therapy, represented genetically engineered oncolytic bacteria, gradually emerged field As only bacterial drug successfully approved oncology use, provided decades experience. By consolidating lessons from successes limitations, hope provide valuable insights application therapies treatment.

Language: Английский

Citations

0
Misidentification of Raoultella spp. (R. terrigena, R. planticola) and Klebsiella spp. (K. variicola, K. grimontii) as Klebsiella pneumoniae: retrospective study of a necropsy-associated bacterial collection from horses DOI Creative Commons
François Gravey,

Corinne Sévin,

Bénédicte Langlois

et al.

Veterinary Microbiology, Journal Year: 2025, Volume and Issue: 304, P. 110497 - 110497

Published: March 30, 2025

Language: Английский

Citations

0