Performance of disk diffusion, gradient test, and VITEK 2 for carbapenem susceptibility testing in OXA-48-like carbapenemase-producing Enterobacterales : a comparative study DOI Creative Commons
Cansu Çimen,

Philipp Siemer,

Janko Sattler

et al.

Journal of Clinical Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

ABSTRACT This study aimed to compare the performance of disk diffusion, gradient test (ETEST), and VITEK 2 (AST-N223, AST-N428, AST-N432 cards) antibiotic susceptibility testing methods with reference broth microdilution (BMD) for carbapenem in OXA-48-like carbapenemase-producing Enterobacterales (CPE). A total 107 CPE 142 controls ( that do not produce any type carbapenemases), all molecularly characterized by whole-genome sequencing, were tested using BMD derivative methods. Essential agreement (EA), categorical (CA), major error, very bias evaluated. In group, resistance frequencies ertapenem, imipenem, meropenem 86.9%, 12.1%, 10.3%, respectively. For CPE, ETEST showed highest EA among (100/107, 93.5%) ertapenem (99/107, 92.5%), while cards <90%. contrast, AST-N428 performed best an 95/105 (90.5%). CA was higher across (93.5%–98.1%) compared imipenem (59.8%–81.4%) (78.8%–95.3%). The achieved meropenem, AST-N223 imipenem. Significant variability observed different tests frequencies, MICs, EA, group. Ertapenem most useful detecting Laboratories should be aware leads more erroneous results other carbapenems when Additionally, tend overcall CPE. IMPORTANCE is frequent carbapenemase western Europe, both its rapid spread challenging-to-detect nature are a particular concern adequate treatment infection control purposes. Accurate determination minimal inhibitory concentrations (MICs) utmost importance, selection therapy as marker detection. However, unclear isolates. Our reports on varying OXA-48-like-producing . present can help inform about limitations current serve improve MIC these challenging

Language: Английский

Rapidly spreading Enterobacterales with OXA-48-like carbapenemases DOI Creative Commons

Gisele Peirano,

Johann Pitout

Journal of Clinical Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

ABSTRACT Enterobacterales (mostly Klebsiella pneumoniae , Escherichia coli ) with OXA-48-like carbapenemases (e.g., OXA-48, -181, -232, -244) are undermining the global efficiency of carbapenem therapy. In Middle East, North Africa, and some European countries, most common types among . Currently, OXA-48 is endemic in Spain, France, Belgium; OXA-181 Sub-Saharan Africa Indian Subcontinent, while OXA-232 has been increasing Subcontinent. countries Germany, Denmark, Switzerland, France) experiencing community outbreaks E. ST38 that produce OXA-244, these strains have introduced into Norwegian, Polish, Czech hospitals. The ascendancy genes due to combination horizontal spread through promiscuous plasmids IncL, IncX3, ColE2) vertical certain high-risk multidrug-resistant clones K. ST14, ST15, ST147, ST307; ST38, ST410). This a powerful “gene survival strategy” assisted different environments including setting. laboratory diagnosis complex; therefore, bacteria “difficult detect” variants OXA-484) likely underreported spreading silently “beneath radar” hospital settings. forces be reckoned with.

Language: Английский

Citations

2
Fluorescence Immunochromatographic Assay Based on Cdse/Zns Quantum Dots for the Detection of Oxa-48 DOI
Aiping Wang,

Mi Lun,

Yumei Chen

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Performance of disk diffusion, gradient test, and VITEK 2 for carbapenem susceptibility testing in OXA-48-like carbapenemase-producing Enterobacterales : a comparative study DOI Creative Commons
Cansu Çimen,

Philipp Siemer,

Janko Sattler

et al.

Journal of Clinical Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

ABSTRACT This study aimed to compare the performance of disk diffusion, gradient test (ETEST), and VITEK 2 (AST-N223, AST-N428, AST-N432 cards) antibiotic susceptibility testing methods with reference broth microdilution (BMD) for carbapenem in OXA-48-like carbapenemase-producing Enterobacterales (CPE). A total 107 CPE 142 controls ( that do not produce any type carbapenemases), all molecularly characterized by whole-genome sequencing, were tested using BMD derivative methods. Essential agreement (EA), categorical (CA), major error, very bias evaluated. In group, resistance frequencies ertapenem, imipenem, meropenem 86.9%, 12.1%, 10.3%, respectively. For CPE, ETEST showed highest EA among (100/107, 93.5%) ertapenem (99/107, 92.5%), while cards <90%. contrast, AST-N428 performed best an 95/105 (90.5%). CA was higher across (93.5%–98.1%) compared imipenem (59.8%–81.4%) (78.8%–95.3%). The achieved meropenem, AST-N223 imipenem. Significant variability observed different tests frequencies, MICs, EA, group. Ertapenem most useful detecting Laboratories should be aware leads more erroneous results other carbapenems when Additionally, tend overcall CPE. IMPORTANCE is frequent carbapenemase western Europe, both its rapid spread challenging-to-detect nature are a particular concern adequate treatment infection control purposes. Accurate determination minimal inhibitory concentrations (MICs) utmost importance, selection therapy as marker detection. However, unclear isolates. Our reports on varying OXA-48-like-producing . present can help inform about limitations current serve improve MIC these challenging

Language: Английский

Citations

0