Diagnosis, Severity, and Prognosis from Potential Biomarkers of COVID-19 in Urine: A Review of Clinical and Omics Results DOI Creative Commons
Jennifer Narro-Serrano, Frutos C. Marhuenda‐Egea

Metabolites, Journal Year: 2024, Volume and Issue: 14(12), P. 724 - 724

Published: Dec. 22, 2024

The COVID-19 pandemic, caused by the SARS-CoV-2 virus, has spurred an extraordinary scientific effort to better understand disease’s pathophysiology and develop diagnostic prognostic tools guide more precise effective clinical management. Among biological samples analyzed for biomarker identification, urine stands out due its low risk of infection, non-invasive collection, suitability frequent, large-volume sampling. Integrating data from omics studies with standard biochemical analyses offers a deeper comprehensive understanding COVID-19. This review aims provide detailed summary published date that have applied on identify potential biomarkers In July 2024, advanced search was conducted in Web Science using query: “covid* (Topic) AND metabol* (Topic)”. included results up 14 October 2024. retrieved this digital were evaluated through two-step screening process: first reviewing titles abstracts eligibility, then retrieving assessing full texts articles met specific criteria. initial 913 studies, which 45 ultimately review. most robust identified include kynurenine, neopterin, total proteins, red blood cells, ACE2, citric acid, ketone bodies, hypoxanthine, amino acids, glucose. causes underlying these alterations reflect multisystemic impact COVID-19, highlighting key processes such as systemic inflammation, renal dysfunction, critical hypoxia, metabolic stress.

Language: Английский

Effectiveness and user experience of nose and throat swabbing techniques for SARS-CoV-2 detection: results from the UK COVID-19 National Testing Programme DOI Creative Commons
Matthias E. Futschik, Raghavendran Kulasegaran‐Shylini, Edward Blandford

et al.

BMC Global and Public Health, Journal Year: 2025, Volume and Issue: 3(1)

Published: Jan. 13, 2025

Abstract Background The UK’s National Health Service Test and Trace (NHSTT) program aimed to provide the most effective accessible SARS-CoV-2 testing approach possible. Early user feedback indicated that there were accessibility issues associated with throat swabbing. We report results of service evaluations performed by NHSTT assess effectiveness acceptance swabbing approaches, as well qualitative findings experiences from research reports, surveys, incident reports. Our intent is present summarize our about application alternative approaches during COVID-19 pandemic in UK. Methods From May 2020 December 2021, conducted a series assessing self-swabbing assisted nose throat, only (anterior nares/mid-turbinate) using polymerase chain reaction (PCR) lateral flow devices (LFDs), for diagnostic suitability within Testing Programme. Outcomes included observational on quantitative performance (concordance, sensitivity, specificity). A post-hoc indirect comparison was also performed. Additionally, an analysis existing cross-service April 2021 determine regarding approaches. Results Observational data preference over Significantly more users reported easier perform than (50% vs. 12%) significantly fewer incidents. In evaluations, while reduced sensitivity nose-only PCR (88%) compared swabbing, similar sensitivities observed LFDs. LFDs higher individuals viral concentrations. Conclusions User experience analyses supported Nose-only provided sufficient accuracy, supporting its use viable option Less invasive are important maximize alongside other behavioral interventions, increase uptake.

Language: Английский

Citations

1
Multiplex lateral flow test sensitivity and specificity in detecting influenza A, B and SARS-CoV-2 in adult patients in a UK emergency department DOI Creative Commons
Rahul Batra, Edward Blandford, Raghavendran Kulasegaran‐Shylini

et al.

Emergency Medicine Journal, Journal Year: 2025, Volume and Issue: unknown, P. emermed - 214177

Published: Jan. 14, 2025

Background Rapid identification of individuals with acute respiratory infections is crucial for preventing nosocomial infections. For rapid diagnosis, especially in EDs, lateral flow devices (LFDs) are a convenient, inexpensive option turnaround. Several ‘multiplex’ LFDs (M-LFDs) now exist, testing multiple pathogens from single swab sample. We evaluated the real-world performance M-LFD versus PCR detecting influenza A, B and SARS-CoV-2) ED setting. Methods After preliminary evaluation an (SureScreen) laboratory-grown virus PCR-negative clinical samples, it was setting at St Thomas’ Hospital (London, UK) 1 December 2022 to 21 April 2023. Eligible participants were ≥18 years age, admitted symptoms received concurrent tests. Main endpoints sensitivity detect A/B (primary) SARS-CoV-2 (secondary) PCR. The probability true positive relation viral concentration (expressed as cycle threshold (Ct)) analysed using logistic regression. Results In total, 808 symptomatic included (49.8% female; mean age 46.9 years). Test (95% CI) 67.0% (56.9% 76.1%) A (n=100), 94.1% (71.3% 99.9%) (n=17) 48.2% (39.7% 56.8%) (n=141). Sensitivity significantly lower than that (p=0.0057 p=0.00088, respectively). 98% Ct<25 (influenza non-evaluable). No co-infections identified by or M-LFD. Conclusion SureScreen consistent laboratory achieved high concentration, most likely be infectious. Given representative UK population sample, results could generalised use other settings.

Language: Английский

Citations

0
Association between confirmed COVID-19 cases at hospitals and SARS-CoV-2 levels in municipal wastewater during the pandemic and endemic phases DOI Creative Commons
Keisuke Kagami, Masaaki Kitajima, Haruhisa Watanabe

et al.

Environment International, Journal Year: 2025, Volume and Issue: 197, P. 109342 - 109342

Published: Feb. 20, 2025

Language: Английский

Citations

0
Key SARS-CoV-2 testing strategies implemented in the UK: rationale and impact DOI Creative Commons
Tom Fowler,

Chris Kenny,

Sarah Tunkel

et al.

Journal of the Royal Society of Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Language: Английский

Citations

0
Clinical Significance of Neutralizing Antibodies in COVID-19: Implications for Disease Prognosis DOI Creative Commons
Sudem Mahmutoğlu Çolak, Tuba İlgar, İlkay Bahçeci

et al.

Life, Journal Year: 2025, Volume and Issue: 15(3), P. 429 - 429

Published: March 8, 2025

The pathogenesis of COVID-19 highlights a complex relationship between disease severity and neutralizing antibodies (NAbs). We aimed to investigate the relationships among total NAb (tNAb) levels, presence potential neutralization (pNAbs), clinical outcomes patients. Patients aged ≥18 years diagnosed October 2021 September 2022 were grouped by symptom severity. Blood samples taken at two time points data on demographics, epidemiology, vaccination recorded. tNAbs pNAbs measured an enhanced chemiluminescence assay surrogate virus test, respectively. 68 52 patients analyzed. Twenty-six (38.2%) had severe infection. 28-day mortality rate was 16.2% (n = 11). tNAb levels in control blood (CBSs) significantly higher than those admission (ABSs) (p < 0.001). statistical analysis showed no pNAbs. Vaccinated ABSs CBSs 0.001 p 0.001, respectively). correlated with lower 0.026) milder course 0.041). Although these findings support correlation mortality, their seems be unrelated favorable outcomes.

Language: Английский

Citations

0
Learn from the SARS-CoV-2 nucleic acid test to increase the experience of dealing with the “disease X “ DOI Creative Commons
Xin Yang, Jinming Li

BMC Infectious Diseases, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 24, 2025

RT‒PCR is crucial for screening epidemic diseases such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, false positives further plague prevention and control. This study conducted a stratified on the initial Ct values positive ratios, providing experience reference addressing future "Disease X". Data from 1,255 or suspected results were obtained eleven laboratories with seven different reagents. The proportion of was analyzed basis among reagents various testing institutions. When both target genes in detection < 30, considered small probability event (≤ 1.72%). when 30 ≤ value 35, significant differences noted (0%, 1.41%, 7.69%, 9.14%, P 0.001). any gene > 15.58 - 24.22% may be positive. Among samples, 53.23% according to retesting. After separate sampling, 4 tubes (30 people involved) 19 (133 negative. In summary, strategies should adopted primary under pandemic control conditions, which provide better rapid diagnosis next

Language: Английский

Citations

0
Diagnosis, Severity, and Prognosis from Potential Biomarkers of COVID-19 in Urine: A Review of Clinical and Omics Results DOI Creative Commons
Jennifer Narro-Serrano, Frutos C. Marhuenda‐Egea

Metabolites, Journal Year: 2024, Volume and Issue: 14(12), P. 724 - 724

Published: Dec. 22, 2024

The COVID-19 pandemic, caused by the SARS-CoV-2 virus, has spurred an extraordinary scientific effort to better understand disease’s pathophysiology and develop diagnostic prognostic tools guide more precise effective clinical management. Among biological samples analyzed for biomarker identification, urine stands out due its low risk of infection, non-invasive collection, suitability frequent, large-volume sampling. Integrating data from omics studies with standard biochemical analyses offers a deeper comprehensive understanding COVID-19. This review aims provide detailed summary published date that have applied on identify potential biomarkers In July 2024, advanced search was conducted in Web Science using query: “covid* (Topic) AND metabol* (Topic)”. included results up 14 October 2024. retrieved this digital were evaluated through two-step screening process: first reviewing titles abstracts eligibility, then retrieving assessing full texts articles met specific criteria. initial 913 studies, which 45 ultimately review. most robust identified include kynurenine, neopterin, total proteins, red blood cells, ACE2, citric acid, ketone bodies, hypoxanthine, amino acids, glucose. causes underlying these alterations reflect multisystemic impact COVID-19, highlighting key processes such as systemic inflammation, renal dysfunction, critical hypoxia, metabolic stress.

Language: Английский

Citations

0