PNAS Nexus,
Journal Year:
2024,
Volume and Issue:
3(11)
Published: Oct. 25, 2024
Abstract
UDP-α-D-galactofuranose
(UDP-Galf):
β-galactofuranoside
β-(1→5)-galactofuranosyltransferase,
known
as
GfsA,
is
essential
in
synthesizing
β-(1→5)-galactofuranosyl
oligosaccharides
that
are
incorporated
into
the
cell
wall
of
pathogenic
fungi.
This
study
analyzed
structure
and
function
GfsA
from
Aspergillus
fumigatus.
To
provide
crucial
insights
catalytic
mechanism
substrate
recognition,
complex
was
elucidated
with
manganese
(Mn2+),
a
donor
product
(UDP),
an
acceptor
sugar
molecule
(β-galactofuranose).
In
addition
to
typical
GT-A
fold
domain,
has
unique
domain
formed
by
N
C
termini.
The
former
interacts
another
forming
dimer.
active
center
contains
Mn2+,
UDP,
galactofuranose
forms
groove
highly
conserved
Pezizomycotina
Enzymatic
assays
using
site-directed
mutants
were
conducted
determine
roles
specific
active-site
residues
enzymatic
activity
GfsA.
predicted
enzyme–substrate
model
containing
UDP-Galf
characterized
β-galactofuranosyltransfer
5ʹ-OH
β-galactofuranose.
Overall,
fungi
provides
glycan
biosynthetic
processes
fungal
pathogenesis
may
inform
development
novel
antifungal
therapies.
BMC Infectious Diseases,
Journal Year:
2024,
Volume and Issue:
24(1)
Published: June 12, 2024
Several
antifungal
agents
are
available
for
primary
therapy
in
patients
with
invasive
aspergillosis
(IA).
Although
a
few
studies
have
compared
the
effectiveness
of
different
treating
IA,
there
has
yet
to
be
definitive
agreement
on
best
choice.
Herein,
we
perform
network
meta-analysis
comparing
efficacy
IA.
Methods,
Journal Year:
2024,
Volume and Issue:
230, P. 59 - 67
Published: July 22, 2024
Most
bacterial,
plant
and
fungal
cells
possess
at
their
surface
a
protective
layer
called
the
cell
wall,
conferring
strength,
plasticity
rigidity
to
withstand
osmotic
pressure.
This
molecular
barrier
is
crucial
for
pathogenic
microorganisms,
as
it
protects
from
local
environment
often
constitutes
first
structural
component
encountered
in
host-pathogen
interaction.
In
molds
yeasts,
wall
main
target
development
of
clinically-relevant
antifungal
drugs.
past
decade,
solid-state
NMR
has
emerged
powerful
analytical
technique
investigate
organization
microbial
walls
context
intact
cells.
Pharmaceutics,
Journal Year:
2025,
Volume and Issue:
17(1), P. 125 - 125
Published: Jan. 17, 2025
The
development
of
resistance
to
traditional
antifungal
therapies
has
necessitated
the
exploration
alternative
treatment
strategies
effectively
manage
fungal
infections,
particularly
those
induced
by
Candida
albicans
(C.
albicans).
This
research
investigates
possibility
integrating
silver
nanoparticles
(AgNPs)
with
Terbinafine
improve
effectiveness.
Terbinafine,
while
potent,
faces
challenges
specific
strains,
highlighting
need
for
enhance
its
efficacy.
Silver
were
produced
through
a
light-activated,
gelatin-based
method,
resulting
in
particle
sizes
ranging
from
56.8
nm
66.2
nm,
confirmed
dynamic
light
scattering
and
scanning
electron
microscopy.
Stability
studies
indicated
that
AgNPs
30
mg
nitrate
(AgNO₃)
exhibited
greatest
stability
over
60
days
across
different
temperature
conditions.
analysis
UV-visible
spectrophotometry
revealed
notable
shift
absorption
spectra
as
AgNO₃
concentrations
increased,
which
was
associated
strengthening
plasmon
resonance.
effectiveness
combination
assessed
against
three
strains
C.
(ATCC
10231,
ATCC
90028,
18804).
demonstrated
strong
properties
minimum
inhibitory
(MIC)
values
2-4
µg/mL,
whereas
on
their
own
displayed
moderate
integrated
formulation
notably
enhanced
effectiveness,
especially
strain
revealing
synergistic
effect
(FIFi
=
0.369).
These
results
complemented
findings
time-to-kill
assay,
where
same
showed
3.2
log₁₀
CFU/mL
decrease
viable
cell
count.
process
boost
activity
entails
disruption
membrane
internal
components,
probably
result
ion
release
generation
free
radicals.
indicate
may
act
powerful
addressing
resistant
presenting
an
encouraging
direction
future
treatments.
Future Microbiology,
Journal Year:
2025,
Volume and Issue:
unknown, P. 1 - 11
Published: Jan. 27, 2025
Trichosporon
asahii
(T.
asahii)
is
an
opportunistic
pathogenic
fungus
that
often
causes
severe
infections
in
immunosuppressed
patients.
Among
species,
T.
the
most
and
lethal
species.
Current
research
faces
challenges
related
to
unknown
mechanisms,
complex
resistance
insufficiently
rapid
accurate
diagnostic
methods,
insufficient
on
susceptibility
infection.
These
issues
need
be
explored
depth.
This
review
summarizes
progress
origin
classification
of
asahii,
its
virulence
factors
epidemiological
characteristics,
infection
modes,
drug
treatment
options,
mechanisms.
Traditional
culture
combined
with
molecular
biology
techniques,
such
as
polymerase
chain
reaction
gene
sequencing,
has
improved
accuracy
speed
detection.
Treatment
relies
mainly
azole
antifungal
drugs
amphotericin
B;
however,
patients
are
facing
problem
resistance.
New
knockout
have
identified
thus
supporting
development
novel
drugs.
In
summary,
in-depth
study
will
aid
developing
more
effective
therapeutic
methods
improve
patient
prognosis.
mBio,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 29, 2025
ABSTRACT
The
emerging
fungal
pathogen
Candida
auris
is
known
for
its
strong
skin
tropism
and
resilience
against
antifungal
disinfection
treatment,
posing
a
significant
challenge
healthcare
units.
Although
efforts
to
identify
the
effectors
of
unique
pathogenic
behavior
have
been
insightful,
role
high-osmolarity
glycerol
(HOG)
pathway
in
this
context
remains
unexplored.
study
by
Shivarathri
co-workers
(R.
Shivarathri,
M.
Chauhan,
A.
Datta,
D.
Das
et
al.,
mBio
15:e02748-24,
2024,
https://doi.org/10.1128/mbio.02748-24
)
sought
address
gap.
This
report
indeed
advances
our
understanding
critical
HOG
C.
pathogenicity
emphasizing
involvement
colonization,
biofilm
formation,
evasion
phagocyte
attack.
