CRISPR/Cas9 therapeutics: progress and prospects

Signal Transduction and Targeted Therapy, Journal Year: 2023, Volume and Issue: 8(1)

Published: Jan. 16, 2023

Abstract Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene-editing technology is the ideal tool of future for treating diseases by permanently correcting deleterious base mutations or disrupting disease-causing genes with great precision and efficiency. A variety efficient Cas9 variants derivatives have been developed to cope complex genomic changes that occur during diseases. However, strategies effectively deliver CRISPR system diseased cells in vivo are currently lacking, nonviral vectors target recognition functions may be focus research. Pathological physiological resulting from disease onset expected serve as identifying factors targeted delivery targets gene editing. Diseases both varied complex, choice appropriate methods different important. Meanwhile, there still many potential challenges identified when targeting CRISPR/Cas9 treatment. This paper reviews current developments three aspects, namely, type, vector, characteristics. Additionally, this summarizes successful examples clinical trials finally describes possible problems associated applications.

Language: Английский

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TRP (transient receptor potential) ion channel family: structures, biological functions and therapeutic interventions for diseases

Signal Transduction and Targeted Therapy, Journal Year: 2023, Volume and Issue: 8(1)

Published: July 5, 2023

Abstract Transient receptor potential (TRP) channels are sensors for a variety of cellular and environmental signals. Mammals express total 28 different TRP channel proteins, which can be divided into seven subfamilies based on amino acid sequence homology: TRPA (Ankyrin), TRPC (Canonical), TRPM (Melastatin), TRPML (Mucolipin), TRPN (NO-mechano-potential, NOMP), TRPP (Polycystin), TRPV (Vanilloid). They class ion found in numerous tissues cell types permeable to wide range cations such as Ca 2+ , Mg Na + K others. responsible various sensory responses including heat, cold, pain, stress, vision taste activated by number stimuli. Their predominantly location the surface, their interaction with physiological signaling pathways, unique crystal structure make TRPs attractive drug targets implicate them treatment diseases. Here, we review history discovery, summarize structures functions family, highlight current understanding role pathogenesis human disease. Most importantly, describe channel-related therapeutic interventions diseases limitations targeting clinical applications.

Language: Английский

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SARS‐CoV‐2 variants and vulnerability at the global level

Journal of Medical Virology, Journal Year: 2022, Volume and Issue: 94(7), P. 2986 - 3005

Published: March 12, 2022

Numerous variants of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic have evolved. Viral may evolve with harmful susceptibility to immunity established existing COVID-19 vaccination. These are more transmissible, induce relatively extreme illness, evasive immunological features, decrease neutralization using antibodies from vaccinated persons, and susceptible re-infection. The Centers for Disease Control Prevention (CDC) has categorized SARS-CoV-2 mutations as interest (VOI), concern (VOC), high consequence (VOHC). At moment, four VOC many been defined require constant observation. This review article summarizes various surfaced special emphasis on VOCs that spreading across world, well several viral mutational impacts how these modifications alter properties virus.

Language: Английский

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Two Years into the COVID-19 Pandemic: Lessons Learned

ACS Infectious Diseases, Journal Year: 2022, Volume and Issue: 8(9), P. 1758 - 1814

Published: Aug. 8, 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly transmissible and virulent human-infecting that emerged in late December 2019 Wuhan, China, causing disease called (COVID-19), which has massively impacted global public health caused widespread disruption to daily life. The crisis by COVID-19 mobilized scientists authorities across the world rapidly improve our knowledge about this devastating disease, shedding light on its management control, spawned development of new countermeasures. Here we provide an overview state art gained last years virus COVID-19, including origin natural reservoir hosts, viral etiology, epidemiology, modes transmission, clinical manifestations, pathophysiology, diagnosis, treatment, prevention, emerging variants, vaccines, highlighting important differences from previously known pathogenic coronaviruses. We also discuss selected key discoveries each topic underline gaps for future investigations.

Language: Английский

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Fast and sensitive CRISPR detection by minimized interference of target amplification

Nature Chemical Biology, Journal Year: 2024, Volume and Issue: 20(7), P. 885 - 893

Published: Feb. 8, 2024

Language: Английский

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Prevalence of cardiovascular symptoms in post-acute COVID-19 syndrome: a meta-analysis

BMC Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 6, 2025

Since its emergence in 2019, COVID-19 has continued to pose significant threats both the physical and mental health of global population, as well healthcare systems worldwide (Raman et al., Eur Heart J 43:1157–1172, 2022). Emerging evidence indicates that may lead post-acute syndrome (PACS) with cardiovascular implications, potentially driven by factors such ACE2 interaction viruses, systemic inflammation, endothelial dysfunction. However, there remains a limited amount research on manifestations PACS, which delay development optimal treatment strategies for affected patients. Therefore, it is crucial investigate prevalence sequelae patients determine whether infection acts an independent risk factor these outcomes. This meta-analysis adhered PRISMA guidelines was registered PROSPERO (CRD42024524290). A systematic search PubMed, Embase, Cochrane Library conducted up March 17, 2024. The primary outcomes included hypertension, palpitations, chest pain, pooled effect estimate reported proportions odds ratios (ORs) 95% confidence intervals (CIs). Sensitivity subgroup analysis were performed assess robustness results identify sources heterogeneity. total 37 studies, encompassing 2,965,467 patients, analysis. Pooled from case–control studies revealed that, compared control group, ORs pain group 4.0 (95% CI: 1.6, 10.0). palpitation hypertension 3.4 1.1, 10.2) 1.7 1.8), respectively. PACS experiencing palpitation, 22% 14%, 33%), 18% 13%, 24%), 19% 12%, 31%), Our findings indicate 15% experience sequelae. Furthermore, significantly increases likelihood developing uninfected individuals. Future should prioritize investigating underlying pathological mechanisms targeted preventive management strategies. CRD42024524290.

Language: Английский

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Digital PCR Applications in the SARS-CoV-2/COVID-19 Era: a Roadmap for Future Outbreaks

Clinical Microbiology Reviews, Journal Year: 2022, Volume and Issue: 35(3)

Published: March 8, 2022

The ongoing coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2), has led to a global public health disaster. current gold standard for the diagnosis of infected patients is real-time reverse transcription-quantitative PCR (RT-qPCR). As effective as this method may be, it subject false-negative and -positive results, affecting its precision, especially detection low viral loads in samples. In contrast, digital (dPCR), third generation PCR, been shown be more than standard, RT-qPCR, detecting review article, we selected publications show broad-spectrum applications dPCR, including development assays reference standards, environmental monitoring, mutation detection, clinical SARS-CoV-2, while comparing analytically RT-qPCR. summary, evident that specificity, sensitivity, reproducibility, limits RT-dPCR are generally unaffected common factors affect first time dPCR being tested an outbreak such magnitude, knowledge will help chart course future monitoring infectious outbreaks.

Language: Английский

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PLGA-Based Micro/Nanoparticles: An Overview of Their Applications in Respiratory Diseases

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(5), P. 4333 - 4333

Published: Feb. 22, 2023

Respiratory diseases, such as asthma and chronic obstructive pulmonary disease (COPD), are critical areas of medical research, millions people affected worldwide. In fact, more than 9 million deaths worldwide were associated with respiratory diseases in 2016, equivalent to 15% global deaths, the prevalence is increasing every year population ages. Due inadequate treatment options, treatments for many limited relieving symptoms rather curing disease. Therefore, new therapeutic strategies urgently needed. Poly (lactic-co-glycolic acid) micro/nanoparticles (PLGA M/NPs) have good biocompatibility, biodegradability unique physical chemical properties, making them one most popular effective drug delivery polymers. this review, we summarized synthesis modification methods PLGA M/NPs their applications (asthma, COPD, cystic fibrosis (CF), etc.) also discussed research progress current status diseases. It was concluded that promising vehicles due advantages low toxicity, high bioavailability, loading capacity, plasticity modifiability. And at end, presented an outlook on future directions, aiming provide some ideas directions hopefully promote widespread application clinical treatment.

Language: Английский

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Bone/cartilage organoid on-chip: Construction strategy and application

Bioactive Materials, Journal Year: 2023, Volume and Issue: 25, P. 29 - 41

Published: Jan. 20, 2023

The necessity of disease models for bone/cartilage related disorders is well-recognized, but the barrier between ex-vivo cell culture, animal and real human body has been pending decades. organoid-on-a-chip technique showed opportunity to revolutionize basic research drug screening diseases like osteoporosis arthritis. organoid on-chip (BCoC) system a novel platform multi-tissue which faithfully emulate essential elements, biologic functions pathophysiological response under circumstances. In this review, we propose concept BCoC platform, summarize modules current efforts orchestrate them on single microfluidic system. Current models, unsolved problems future challenging are also discussed, aim should be deeper understanding diseases, ultimate realization generic tools further therapeutic strategies pathological conditions.

Language: Английский

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Clinical applications of plasma proteomics and peptidomics: Towards precision medicine

PROTEOMICS - CLINICAL APPLICATIONS, Journal Year: 2022, Volume and Issue: 16(6)

Published: May 1, 2022

In the context of precision medicine, disease treatment requires individualized strategies based on underlying molecular characteristics to overcome therapeutic challenges posed by heterogeneity. For this purpose, it is essential develop new biomarkers diagnose, stratify, or possibly prevent diseases. Plasma an available source that greatly reflects physiological and pathological conditions body. An increasing number studies are focusing proteins peptides, including many involving Human Proteome Project (HPP) Organization (HUPO), proteomics peptidomics techniques emerging as critical tools for developing novel medicine preventative measures. Excitingly, plasma toolbox exhibits a huge potential studying pathogenesis diseases (e.g., COVID-19 cancer), identifying valuable improving clinical management. However, enormous complexity wide dynamic range makes proteome profiling challenging. Herein, we summarize recent advances in with focus their roles cancer research, aiming emphasize significance applications medicine.

Language: Английский

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