Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: Oct. 3, 2024
Language: Английский
British Journal of Biomedical Science, Journal Year: 2024, Volume and Issue: 81
Published: Aug. 7, 2024
The global issue of antimicrobial resistance poses significant challenges to public health. World Health Organization (WHO) has highlighted it as a major health threat, causing an estimated 700,000 deaths worldwide. Understanding the multifaceted nature antibiotic is crucial for developing effective strategies. Several physiological and biochemical mechanisms are involved in development resistance. Bacterial cells may escape bactericidal actions drugs by entering physiologically dormant state known bacterial persistence. Recent findings this field suggest that persistence can be one main sources chronic infections. tolerance developed persister could tolerate high levels antibiotics give rise offspring. These offspring attributed mechanisms, especially This review attempts shed light on persister-induced current therapeutic
Language: Английский
Citations
9
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: April 24, 2025
Abstract Antibiotic resistance is an escalating global concern, necessitating the development of novel antibiotics with unique mechanisms action, and preferably also a lowered propensity for development. Type-I Toxin-Antitoxin (TA) systems that are ubiquitous in bacterial genomes consist genetic toxin element encoding hydrophobic peptide antitoxin producing sRNA inhibits translation. Although biological roles these membrane-associated toxins remain incompletely understood, their inherent lethality upon overexpression suggests potential as antimicrobial agents. In this study, we explore ShoB from shoB-ohsC TA system Escherichia coli ( E. ) basis designing synthetic peptides exogenous delivery. We demonstrate ShoB-derived can retain efficacy when modified into shorter, cationic analogs enhanced solubility. Our most promising hits exhibit rapid bactericidal action frequency within cultures indicate limited tendency These findings highlight type-I constitute source peptide-based antibiotics, thereby offering alternative largely unexplored strategy to combat antibiotic-resistant infections.
Language: Английский
Citations
0
mSystems, Journal Year: 2024, Volume and Issue: 9(11)
Published: Oct. 8, 2024
ABSTRACT Bacterial dormancy is a valuable strategy to survive stressful conditions. Toxins from chromosomal toxin-antitoxin systems have the potential halt cell growth, induce dormancy, and eventually promote stress-tolerant persister state. Due their toxicity when overexpressed, sophisticated expression are needed studying toxin genes. Here, we present moderate system for genes based on an artificial 5′ untranslated region. We applied of gene tisB type I tisB/istR-1 in Escherichia coli . TisB small hydrophobic protein that targets inner membrane, resulting depolarization ATP depletion. analyzed TisB-producing cells by RNA-sequencing revealed several with role recovery TisB-induced including chaperone ibpAB spy The importance suggested prone aggregation, which was validated vivo fluorescent reporter system. moved show essential factor aggregation upon DNA damage mediated fluoroquinolone antibiotic ciprofloxacin E. wild-type cells. occurrence aggregates correlates extended duration, underscores life cycle TisB-dependent IMPORTANCE Protein occur all living due misfolding proteins. In bacteria, associated cellular inactivity, related tolerance conditions, exposure antibiotics. , membrane important ciprofloxacin. provokes which, turn, promotes state dormancy. Our study suggests consequence toxins affect duration outcome therapy.
Language: Английский
Citations
2
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: Oct. 3, 2024
Language: Английский
Citations
0