Providencia pseudovermicola sp. nov.: redefining Providencia vermicola and unveiling multidrug-resistant strains from diabetic foot ulcers in Egypt DOI Creative Commons
Samira M. Hamed, Manal Darwish,

Reham Monir

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 23, 2025

Abstract Background Providencia species are concerning due to their intrinsic resistance colistin and tigecycline, complicating the treatment of multidrug-resistant (MDR) infections. Methods In current study, two MDR isolates, DFU6 DFU52 T , were recovered from infected diabetic foot ulcers in Egypt 2024. Following initial identification as stuartii using VITEK® 2 MALDI-TOF-MS, isolates subjected whole-genome sequencing via DNBseq. Results While 16S rRNA gene showed 100% similarity that vermicola phylogenomic analysis against type strains TYGS database, including P. DSM 17385 confirmed these represent a distinct within genus, further supported by overall genome-relatedness indices (ORGIs). This discrepancy prompted us revise taxonomy all published genomes ( n = 59) which revealed misidentification at least 56 unrelated strain this species. carried novel sequence types (ST29 ST41, submitted PubMLST) harbored multiple genes. Both contained qnrD1 on small, non-mobilizable plasmid. possessed conjugative plasmid encoding bla CMY−6 NDM−1 rmtC aac(6’)-Ib10 sul1 aph(3’)-Ia qacEΔ1 . an IS Ecp 1-associated CTX−M−14 along with aadA dfrA1 lnuF class integron, armA msrE mphE also featured pathogenicity island (PAI) integrated into pheV fimbriae-encoding Conclusion our reassessment taxonomic classification genomes, we propose reclassifying certain strains, for name pseudovermicola sp. nov. We recommend (= CCASU-2024-72) shed light public health threat human pathogen harbours carbapenem aminoglycoside genes mobile genetic elements.

Language: Английский

On the priority between Yokenella regensburgei Kosako et al. 1985 and Koserella trabulsii Hickman-Brenner et al. 1985 and on the priority between Providencia alcalifaciens (de Salles Gomes 1944) Ewing 1962 (Approved Lists 1980) and Proteus inconstans (Ornstein 1920) Shaw and Clarke 1955 (Approved Lists 1980). Requests for an Opinion DOI
Meng‐Syun Li

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2025, Volume and Issue: 75(4)

Published: April 8, 2025

In this paper, I include two Requests for an Opinion regarding ambiguous priority of pairs names considered synonyms. First, present a case unknown between heterotypic synonyms Yokenella regensburgei Kosako et al . 1985 and Koserella trabulsii Hickman-Brenner 1985. These appeared on the same Validation List but without any sequence number given. According to Rule 24b(4) 2022 Revision International Code Nomenclature Prokaryotes (ICNP), in no numbers given, it is up those who first propose union taxa determine priority. However, choice was made by synonymized these names, therefore, impossible choose correct name. To resolve extremely rare, awkward situation, argue that Judicial Commission should conserve genus name epithet well-known Y. over less-known K. according 56b ICNP. And doing this, will become united taxon with particular circumscription. Secondly, discussed which Providencia alcalifaciens (de Salles Gomes 1944) Ewing 1962 (Approved Lists 1980) Proteus inconstans (Ornstein 1920) Shaw Clarke 1955 are found be homotypic both included Approved Lists. Based priority, P. earliest adopted name, means treated as illegitimate epithet. Since proposed type species , generic its combinations well Rules 20a 51b(2) Literature search showed has obsolete prevailing usage. Moreover, 16 validly published including synonyms, have been date under Accordingly, necessary conservation sake nomenclatural stability.

Language: Английский

Citations

0

Providencia pseudovermicola sp. nov.: redefining Providencia vermicola and unveiling multidrug-resistant strains from diabetic foot ulcers in Egypt DOI Creative Commons
Samira M. Hamed, Manal Darwish,

Reham Monir

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 23, 2025

Abstract Background Providencia species are concerning due to their intrinsic resistance colistin and tigecycline, complicating the treatment of multidrug-resistant (MDR) infections. Methods In current study, two MDR isolates, DFU6 DFU52 T , were recovered from infected diabetic foot ulcers in Egypt 2024. Following initial identification as stuartii using VITEK® 2 MALDI-TOF-MS, isolates subjected whole-genome sequencing via DNBseq. Results While 16S rRNA gene showed 100% similarity that vermicola phylogenomic analysis against type strains TYGS database, including P. DSM 17385 confirmed these represent a distinct within genus, further supported by overall genome-relatedness indices (ORGIs). This discrepancy prompted us revise taxonomy all published genomes ( n = 59) which revealed misidentification at least 56 unrelated strain this species. carried novel sequence types (ST29 ST41, submitted PubMLST) harbored multiple genes. Both contained qnrD1 on small, non-mobilizable plasmid. possessed conjugative plasmid encoding bla CMY−6 NDM−1 rmtC aac(6’)-Ib10 sul1 aph(3’)-Ia qacEΔ1 . an IS Ecp 1-associated CTX−M−14 along with aadA dfrA1 lnuF class integron, armA msrE mphE also featured pathogenicity island (PAI) integrated into pheV fimbriae-encoding Conclusion our reassessment taxonomic classification genomes, we propose reclassifying certain strains, for name pseudovermicola sp. nov. We recommend (= CCASU-2024-72) shed light public health threat human pathogen harbours carbapenem aminoglycoside genes mobile genetic elements.

Language: Английский

Citations

0