The two-component system ArlRS is essential for wall teichoic acid glycoswitching in Staphylococcus aureus DOI Creative Commons
Marieke M. Kuijk,

Emma Tusveld,

Esther Lehmann

et al.

mBio, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 29, 2024

ABSTRACT Staphylococcus aureus is among the leading causes of hospital-acquired infections. Critical to S. biology and pathogenesis are cell wall-anchored glycopolymers wall teichoic acids (WTA). Approximately one-third isolates decorates WTA with a mixture α1,4- β1,4- N -acetylglucosamine (GlcNAc), which requires dedicated glycosyltransferases TarM TarS, respectively. Environmental conditions, such as high salt concentrations, affect abundance ratio β1,4-GlcNAc decorations, thereby impacting biological properties antibody binding phage infection. To identify regulatory mechanisms underlying glycoswitching, we screened 1,920 S . mutants (Nebraska Transposon Mutant Library) by immunoblotting for differential expression WTA-linked or using specific monoclonal Fab fragments. Three two-component systems (TCS), GraRS, ArlRS, AgrCA, were 230 potential hits. Using isogenic TCS mutants, demonstrated that ArlRS essential decoration. repressed tarM through transcriptional regulator MgrA. In bacteria lacking arlRS , increased correlated absence β1,4-GlcNAc, likely outcompeting TarS enzymatic activity. was responsive Mg 2+ but not Na + revealing its role in previously reported salt-induced glycoswitch from α1,4-GlcNAc β1,4-GlcNAc. Importantly, ArlRS-mediated regulation glycosylation affected interaction innate receptor langerin lysis β1,4-GlcNAc-dependent phages. Since represents promising target future immune-based treatments vaccines, our findings provide important insight align strategies targeting patterns during IMPORTANCE common colonizer can also cause severe infections humans. The development antibiotic resistance complicates treatment infections, increasing need alternatives vaccines therapies bacterial viruses known Wall (WTA) abundant glycosylated structures have gained attention new treatments. show variation depending on environmental host factors, antibodies pattern-recognition receptors. Here, system involved responding changes concentration. These may support design

Language: Английский

Synergistic removal of Staphylococcus aureus biofilms by using a combination of phage Kayvirus rodi with the exopolysaccharide depolymerase Dpo7 DOI Creative Commons
Ana Catarina Duarte, Lucía Fernández, Andrea Jurado

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 7, 2024

Bacteriophages have been shown to penetrate biofilms and replicate if they find suitable host cells. Therefore, these viruses appear be a good option tackle the biofilm problem complement or even substitute more conventional antimicrobials. However, in order successfully remove biofilms, particular mature phages may need administered along with other compounds. Phage-derived proteins, such as endolysins depolymerases, offer safer alternative compounds era of antibiotic resistance.

Language: Английский

Citations

3
Virulence factors in biofilm formation and therapeutic strategies for Staphylococcus aureus: A review DOI Creative Commons
Dali Wang, Li Wang, Quan Liu

et al.

Animals and zoonoses., Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

Citations

2
O-glycosylation of IgA1 and the pathogenesis of an autoimmune disease IgA nephropathy DOI
Jan Novák, R. Glenn King, Janet Yother

et al.

Glycobiology, Journal Year: 2024, Volume and Issue: 34(11)

Published: Aug. 2, 2024

Abstract IgA nephropathy is a kidney disease characterized by deposition of immune complexes containing abnormally O-glycosylated IgA1 in the glomeruli. Specifically, some O-glycans are missing galactose that normally β1,3-linked to N-acetylgalactosamine core 1 glycans. These galactose-deficient glycoforms produced IgA1-secreting cells due dysregulated expression and activity several glycosyltransferases. Galactose-deficient circulation patients with bound IgG autoantibodies resultant can contain additional proteins, such as complement C3. complexes, if not removed from circulation, enter glomerular mesangium, activate resident mesangial cells, induce injury. In this review, we briefly summarize clinical pathological features nephropathy, review normal aberrant O-glycosylation pathways, discuss origins potential significance natural anti-glycan antibodies, namely those recognizing N-acetylgalactosamine. We also specific for characteristics pathogenic IgG. kidneys injured IgA1-containing innocent bystanders. Most progress failure require dialysis or transplantation. Moreover, most after transplantation experience recurrent disease. Thus, better understanding pathogenetic mechanisms needed develop new disease-specific treatments.

Language: Английский

Citations

1
The two-component system ArlRS is essential for wall teichoic acid glycoswitching inStaphylococcus aureus DOI Creative Commons
Marieke M. Kuijk,

Emma Tusveld,

Esther Lehmann

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Abstract Staphylococcus aureus is among the leading causes of hospital-acquired infections. Critical to S. biology and pathogenesis are cell wall-anchored glycopolymers wall teichoic acids (WTA). Approximately one-third isolates decorates WTA with a mixture α1,4- β1,4- N -acetylglucosamine (GlcNAc), which requires dedicated glycosyltransferases TarM TarS, respectively. Environmental conditions, such as high salt concentrations, affect abundance ratio β1,4-GlcNAc decorations, thereby impacting biological properties antibody binding phage infection. To identify regulatory mechanisms underlying glycoswitching, we screened 1,920 mutants (Nebraska Transposon Mutant Library) by immunoblotting for differential expression WTA-linked or using specific monoclonal Fab fragments. Three two-component systems (TCS), GraRS, ArlRS, AgrCA, were 230 potential hits. Using isogenic TCS mutants, demonstrated that ArlRS essential decoration through regulation tarM but not tarS . regulated transcriptional regulator MgrA, was responsive Mg2+, Na+. Importantly, ArlRS-mediated glycosylation affected interaction innate receptor langerin lysis β1,4-GlcNAc-dependent phages. Since represents promising target future immune-based treatments vaccines, our findings provide important insight align targeting strategies patterns during Importance common colonizer mucosal surfaces, also frequent cause severe infections in humans. Development antibiotic resistance complicates treatment infections, increasing need alternatives vaccines therapies bacterial viruses known Wall (WTA) abundantly-expressed glycosylated structures have gained attention new treatments. show variation depending on environmental host factors, antibodies pattern-recognition receptors. Here, system its effector MgrA involved responding changes Mg 2+ concentration. These may support design

Language: Английский

Citations

0
The two-component system ArlRS is essential for wall teichoic acid glycoswitching in Staphylococcus aureus DOI Creative Commons
Marieke M. Kuijk,

Emma Tusveld,

Esther Lehmann

et al.

mBio, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 29, 2024

ABSTRACT Staphylococcus aureus is among the leading causes of hospital-acquired infections. Critical to S. biology and pathogenesis are cell wall-anchored glycopolymers wall teichoic acids (WTA). Approximately one-third isolates decorates WTA with a mixture α1,4- β1,4- N -acetylglucosamine (GlcNAc), which requires dedicated glycosyltransferases TarM TarS, respectively. Environmental conditions, such as high salt concentrations, affect abundance ratio β1,4-GlcNAc decorations, thereby impacting biological properties antibody binding phage infection. To identify regulatory mechanisms underlying glycoswitching, we screened 1,920 S . mutants (Nebraska Transposon Mutant Library) by immunoblotting for differential expression WTA-linked or using specific monoclonal Fab fragments. Three two-component systems (TCS), GraRS, ArlRS, AgrCA, were 230 potential hits. Using isogenic TCS mutants, demonstrated that ArlRS essential decoration. repressed tarM through transcriptional regulator MgrA. In bacteria lacking arlRS , increased correlated absence β1,4-GlcNAc, likely outcompeting TarS enzymatic activity. was responsive Mg 2+ but not Na + revealing its role in previously reported salt-induced glycoswitch from α1,4-GlcNAc β1,4-GlcNAc. Importantly, ArlRS-mediated regulation glycosylation affected interaction innate receptor langerin lysis β1,4-GlcNAc-dependent phages. Since represents promising target future immune-based treatments vaccines, our findings provide important insight align strategies targeting patterns during IMPORTANCE common colonizer can also cause severe infections humans. The development antibiotic resistance complicates treatment infections, increasing need alternatives vaccines therapies bacterial viruses known Wall (WTA) abundant glycosylated structures have gained attention new treatments. show variation depending on environmental host factors, antibodies pattern-recognition receptors. Here, system involved responding changes concentration. These may support design

Language: Английский

Citations

0