Medicine and ecology,
Journal Year:
2025,
Volume and Issue:
1, P. 30 - 38
Published: May 16, 2025
The
causative
agent
of
varicella
virus
is
widespread.
In
children,
especially
with
a
complicated
premorbid
background,
there
high
incidence
severe
forms
and
complications.
Widespread
use
specific
prophylaxis
necessary.
According
to
WHO
estimates,
4.2
million
registered
cases
complications
chickenpox
resulted
in
4,200
deaths.
Severe
course
virus,
addition
mortality
are
typical
for
newborns
individuals
impaired
immune
system.
following
were
identified
deceased
patients
virus:
bacterial
(55%),
pneumonia
(54%),
hemorrhagic
(41%),
from
the
central
nervous
system
(33%).
genetic
diversity
plays
key
role
its
pathogenicity,
epidemiology
response
vaccination.
Despite
successful
vaccination
programs,
continues
circulate
population,
which
emphasizes
need
deep
understanding
diversity.
Genetic
varicella-zoster
viruses
may
influence
response,
vaccine
efficacy,
making
it
an
important
subject
research.
Global
research
results
this
area
highlight
importance
monitoring
evolution
developing
effective
treatment
strategies.
These
be
useful
epidemiologists,
immunologists,
virologists,
infectious
disease
specialists.
Expert Review of Molecular Diagnostics,
Journal Year:
2024,
Volume and Issue:
24(5), P. 423 - 438
Published: May 3, 2024
Diagnostics
are
an
essential,
undervalued
part
of
the
health-care
system.
For
many
diseases,
molecular
diagnostics
gold
standard,
but
not
easy
to
implement
in
Low-
and
Middle-Income
Countries
(LMIC).
Sample-to-result
(S2R)
platforms
combining
all
procedures
a
closed
system
could
offer
solution.
In
this
paper,
we
investigated
their
suitability
for
implementation
LMIC.
Biosensors,
Journal Year:
2024,
Volume and Issue:
14(6), P. 265 - 265
Published: May 22, 2024
Traditional
single
nucleic
acid
assays
can
only
detect
one
target
while
multiple
targets
simultaneously,
providing
comprehensive
and
accurate
information.
Fluorescent
microspheres
in
multiplexed
detection
offer
high
sensitivity,
specificity,
multiplexing,
flexibility,
scalability
advantages,
enabling
precise,
real-time
results
supporting
clinical
diagnosis
research.
However,
face
challenges
like
complexity,
costs,
sample
handling
issues.
The
review
explores
the
recent
advancements
applications
of
fluorescent
detection.
It
discusses
versatility
various
fields,
such
as
disease
diagnosis,
drug
screening,
personalized
medicine.
highlights
possibility
adjusting
performance
by
modifying
concentrations
carrier
forms,
allowing
for
tailored
applications.
emphasizes
potential
microsphere
technology
revolutionizing
advancing
health,
treatment,
medical
Expert Review of Molecular Diagnostics,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 27, 2025
Rapid
and
accurate
laboratory
diagnosis
is
essential
for
the
effective
treatment
of
bloodstream
infection
(BSI).
This
review
aims
to
address
novel
traditional
approaches
that
exhibit
different
performance
characteristics
in
BSI.
In
particular,
authors
will
discuss
pros
cons
blood
culture-based
phenotypic
methods,
nucleic
acid-targeted
molecular
host
response-targeted
biomarker
detection
manuscript
summarizes
etiologic
host-based
techniques
Both
methods
are
not
mutually
exclusive
but
should
be
selected
based
on
clinical
needs
conditions
help
diagnose
BSI
more
quickly
accurately.
International Journal of Infectious Diseases,
Journal Year:
2025,
Volume and Issue:
unknown, P. 107835 - 107835
Published: Feb. 1, 2025
Highlights•A
HiFi-LAMP
assay
for
M.
leprea
has
a
LOD
of
43
copies/25
μL
reaction•A
higher
detection
rate
leprae
in
nasal
swabs
than
oral
swabs•The
sensitivity
the
qPCR
assay•The
extraction-free
short
sample-to-answer
time
(<30
mins)AbstractBackgroundTimely
and
accurate
Mycobacterium
(M.
leprae)
is
crucial
efficient
treatment
early
intervention
leprosy,
which
requires
simple
rapid
assay.MethodsAn
was
developed
leprae.
The
performance
assessed
by
comparing
with
nested
PCR
assays
using
clinical
samples.
saliva
from
individuals
leprosy.ResultsThe
high
specificity
limit
(LOD)
reaction.
Both
were
100%
130
purified
DNA
samples,
slightly
50-88.9%
assay.
A
observed
swabs.
directly
6
that
11,833
RLEP
copies/mL
can
be
completed
within
30
mins,
showed
66.7%
three
samples
when
compared
DNA.ConclusionThe
standard
and/or
used
detecting
monitoring
endemic
areas
resource-limited
settings.Graphical
Abstract
Ecology and Evolution,
Journal Year:
2025,
Volume and Issue:
15(2)
Published: Feb. 1, 2025
ABSTRACT
Evaluating
field‐sourced
samples
with
poor‐quality
and
low‐quantity
DNA,
like
animal
feces,
presents
significant
challenges
in
the
field
of
molecular
biology.
Nonetheless,
recent
innovations
PCR
technology
are
promoted
as
effective
tools
to
overcome
many
these
issues.
Here,
we
evaluate
efficiency
droplet
digital
(ddPCR)
a
method
for
color
vision
assessment
from
feces
white‐faced
capuchins
(
Cebus
imitator
)
report
frequencies
alleles
genotypes
wild
population.
The
sex‐linked
polymorphism
monkeys
Americas
is
driven
by
single
nucleotide
polymorphisms
(SNPs)
opsin
genes
at
up
three
tuning
sites.
DNA
was
extracted
fecal
collected
211
(53.1%
males)
Sector
Santa
Rosa,
Costa
Rica:
56
were
evaluated
ddPCR,
24
both
ddPCR
Sanger
sequencing,
141
sequencing
(historical
dataset).
same
opsins
derived
each
monkey
using
ddPCR;
however,
latter
far
more
sensitive
required
fewer
reach
definitive
genotype.
Overall,
most
frequent
phenotypes
red
green/red.
distribution
was:
Females
N
=
99):
green/red
(35.4%),
red/red
(33.3%),
green/yellow
(14.1%),
yellow/red
(12.1%),
yellow/yellow
(4.0%),
green/green
(1.0%);
Males
112):
(60.7%),
yellow
(23.2%),
green
(16.1%).
reliable
evaluating
noninvasively
advantage
excellent
sensitivity
high‐throughput.
highly
robust
inhibitors
can
be
potentially
used
identify
other
disease‐related
SNP
mutations
animals.
Microchimica Acta,
Journal Year:
2025,
Volume and Issue:
192(3)
Published: Feb. 12, 2025
Abstract
The
application
is
demonstrated
of
injection-molded
centrifugal
microfluidic
chips
with
integrated
optical
pH
sensors
for
multiplexed
detection
respiratory
syndrome
coronavirus
2
(SARS-CoV-2),
influenza
A,
and
B
RNA.
generated
sensitive
fluorescent
readouts
from
diagnostic
reverse
transcription
loop-mediated
isothermal
amplification
(RT-LAMP)
reactions;
limits
influenzas
A
B,
SARS-CoV-2
89,
245,
38
RNA
copies
per
reaction,
respectively,
were
attained.
Results
obtainable
within
44
min
48
B.
We
implemented
a
data
processing
strategy
based
on
numerical
derivatives
the
fluorescence
curves
that
allowed
reliable,
quantitative
thresholds
deciding
reaction
outcomes
enabled
100%
specificity.
This
work
demonstrates
utility
microfluidics
infectious
disease
diagnostics
point-of-care
applications.
Graphical
