SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9
Cell,
Journal Year:
2023,
Volume and Issue:
186(22), P. 4834 - 4850.e23
Published: Oct. 1, 2023
Regulation
of
viral
RNA
biogenesis
is
fundamental
to
productive
SARS-CoV-2
infection.
To
characterize
host
RNA-binding
proteins
(RBPs)
involved
in
this
process,
we
biochemically
identified
bound
genomic
and
subgenomic
RNAs.
We
find
that
the
protein
SND1
binds
5'
end
negative-sense
required
for
synthesis.
SND1-depleted
cells
form
smaller
replication
organelles
display
diminished
virus
growth
kinetics.
discover
NSP9,
a
RBP
direct
interaction
partner,
covalently
linked
ends
positive-
RNAs
produced
during
These
linkages
occur
at
replication-transcription
initiation
sites,
consistent
with
NSP9
priming
Mechanistically,
remodels
occupancy
alters
covalent
linkage
initiating
nucleotides
RNA.
Our
findings
implicate
synthesis
unravel
an
unsuspected
role
cellular
orchestrating
production.
Language: Английский
Structural and functional insights into the enzymatic plasticity of the SARS-CoV-2 NiRAN domain
Molecular Cell,
Journal Year:
2023,
Volume and Issue:
83(21), P. 3921 - 3930.e7
Published: Oct. 26, 2023
Language: Английский
Targeting cap1 RNA methyltransferases as an antiviral strategy
Cell chemical biology,
Journal Year:
2023,
Volume and Issue:
31(1), P. 86 - 99
Published: Dec. 12, 2023
Methylation
is
one
of
the
critical
modifications
that
regulates
numerous
biological
processes.
Guanine
capping
and
methylation
at
7
Language: Английский
SHIFTR enables the unbiased identification of proteins bound to specific RNA regions in live cells
Nucleic Acids Research,
Journal Year:
2024,
Volume and Issue:
52(5), P. e26 - e26
Published: Jan. 28, 2024
Abstract
RNA-protein
interactions
determine
the
cellular
fate
of
RNA
and
are
central
to
regulating
gene
expression
outcomes
in
health
disease.
To
date,
no
method
exists
that
is
able
identify
proteins
interact
with
specific
regions
within
endogenous
RNAs
live
cells.
Here,
we
develop
SHIFTR
(Selective
RNase
H-mediated
interactome
framing
for
target
regions),
an
efficient
scalable
approach
bound
selected
using
mass
spectrometry.
Compared
state-of-the-art
techniques,
superior
accuracy,
captures
minimal
background
requires
orders
magnitude
lower
input
material.
We
establish
workflows
targeting
classes
different
length
abundance,
including
short
long
non-coding
RNAs,
as
well
mRNAs
demonstrate
compatible
sequentially
mapping
interactomes
multiple
a
single
experiment.
Using
SHIFTR,
comprehensively
cis-regulatory
elements
located
at
5′
3′-terminal
authentic
SARS-CoV-2
infected
cells
accurately
recover
known
novel
linked
function
these
viral
elements.
enables
systematic
region-resolved
any
cell
type
has
potential
revolutionize
our
understanding
transcriptomes
their
regulation.
Language: Английский
Viral RNA capping: Mechanisms and antiviral therapy
Saini Chen,
No information about this author
Zhimin Jiang,
No information about this author
Qiuchen Li
No information about this author
et al.
Journal of Medical Virology,
Journal Year:
2024,
Volume and Issue:
96(5)
Published: April 29, 2024
Abstract
RNA
capping
is
an
essential
trigger
for
protein
translation
in
eukaryotic
cells.
Many
viruses
have
evolved
various
strategies
initiating
the
of
viral
genes
and
generating
progeny
virions
infected
cells
via
synthesizing
cap
structure
or
stealing
from
nascent
host
messenger
ribonucleotide
acid
(mRNA).
In
addition
to
translation,
a
new
understanding
role
antiviral
innate
immunity
has
advanced
field
mRNA
synthesis
vitro
therapeutic
applications.
Recent
studies
on
these
systems
revealed
startlingly
diverse
ways
molecular
machinery.
A
comprehensive
how
accomplish
pivotal
designing
effective
broad‐spectrum
therapies.
Here
we
systematically
review
contemporary
insights
into
RNA‐capping
mechanisms
employed
by
causing
human
animal
infectious
diseases,
while
also
highlighting
its
impact
immune
response.
The
applications
targeting
against
infections
development
inhibitors
are
summarized.
Language: Английский
Viral RNA Interactome: The Ultimate Researcher’s Guide to RNA–Protein Interactions
W. B. Hanson,
No information about this author
Gabriel A. Romero Agosto,
No information about this author
Silvi Rouskin
No information about this author
et al.
Viruses,
Journal Year:
2024,
Volume and Issue:
16(11), P. 1702 - 1702
Published: Oct. 30, 2024
RNA
molecules
in
the
cell
are
bound
by
a
multitude
of
RNA-binding
proteins
(RBPs)
with
variety
regulatory
consequences.
Often,
interactions
these
facilitated
complex
secondary
and
tertiary
structures
molecules.
Viral
RNAs
especially
known
to
be
heavily
structured
interact
many
RBPs,
roles
including
genome
packaging,
immune
evasion,
enhancing
replication
transcription,
increasing
translation
efficiency.
As
such,
RNA-protein
interactome
represents
critical
facet
viral
cycle.
Characterization
is
necessary
for
development
novel
therapeutics
targeted
at
disruption
essential
cycle
events.
In
this
review,
we
aim
summarize
various
shaping
interactome,
interactions,
as
well
up-to-date
methods
developed
characterization
directions
novel,
RNA-directed
therapeutics.
Language: Английский
SHIFTR enables the unbiased and multiplexed identification of proteins bound to specific RNA regions in live cells
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Oct. 10, 2023
ABSTRACT
RNA-protein
interactions
determine
the
cellular
fate
of
RNA
and
are
central
to
regulating
gene
expression
outcomes
in
health
disease.
To
date,
no
method
exists
that
is
able
identify
proteins
interact
with
specific
regions
within
endogenous
RNAs
live
cells.
Here,
we
develop
SHIFTR
(
S
elective
RNase
H
-mediated
interactome
framing
for
target
regions),
an
efficient
scalable
approach
bound
selected
using
mass
spectrometry.
Compared
state-of-the-art
techniques,
superior
accuracy,
captures
close
zero
background
requires
orders
magnitude
lower
input
material.
We
establish
workflows
targeting
classes
different
length
abundance,
including
short
long
non-coding
RNAs,
as
well
mRNAs
demonstrate
compatible
sequentially
mapping
interactomes
multiple
a
single
experiment.
Using
SHIFTR,
comprehensively
cis
-regulatory
elements
located
at
5ʹ
3ʹ-
terminal
authentic
SARS-CoV-2
genome
infected
cells
accurately
recover
known
novel
linked
function
these
viral
elements.
enables
systematic
region-resolved
any
cell
type
has
potential
revolutionize
our
understanding
transcriptomes
their
regulation.
Language: Английский