Study on the immune enhancers against <i>Micropterus salmoides</i> rhabdovirus infection DOI Creative Commons

Lei Ning,

Chaonan Zhang,

Yanchao Wang

et al.

Journal of Limnology, Journal Year: 2024, Volume and Issue: 83

Published: Oct. 11, 2024

Micropterus salmoides rhabdovirus (MSRV) is one of the most serious pathogens harming M. juvenile, which had brought huge economic losses to farming industry. Studies involving candidate genes clinical diseases, however, are limited. In this study, viral target and manifestation MSRV on juvenile were analyzed, protective effects a single immune enhancer compound evaluated. The results showed that brain, liver, intestine muscle obvious lesions after infection with MSRV. relative expression levels nucleoprotein (N) matrix protein (M) trend increasing at first then decreasing reached peak in each tissue 36 h post-infection. mortality rate was over 90% 7 days infection. enhancers containing free nucleotides Astragalus polysaccharide added diet effectively inhibited replication N M increased survival by 25% 28%. This study provided basic data theoretical reference for analysis pathological mechanism prevention treatment

Language: Английский

Dihydroartemisinin inhibits Micropterus salmoides rhabdovirus infection in vitro and in vivo DOI
Yuanyuan Li, Peipei Zhang,

Shuangshuang Mao

et al.

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742163 - 742163

Published: Jan. 1, 2025

Language: Английский

Citations

1
Transcriptome analysis reveals hypoxic response key genes and modules as well as adaptive mechanism of crucian carp (Carassius auratus) gill under hypoxic stress DOI Creative Commons

Meng-Chao Xing,

Zhen Rong, Xin Zhao

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 5, 2025

Fish gill tissue is a primary organ responsive to acute oxygen deprivation or dissolved (DO) fluctuations in aquatic environments. However, the adaptive mechanism of crucian carp hypoxic stress remains largely unknown. Here, we investigated physiological and transcriptomic changes exposed conditions (dissolved concentration 0.6 ± 0.3 mg/L) for different durations (0 d, 1 2d, 3d, 4 5d). Transcriptomic analysis revealed that hypoxia group (0.6 mg/L DO) exhibited reduction interlamellar cell mass (ILCM) on filaments, compared with control (6.6 DO). With prolonged stress, epithelial cells lamellae became sparse at 3 d 5 vacuoles were increased. A total 3,502 differentially expressed genes (DEGs) identified, hypoxia-specific modules screened through differential expression analysis, weighted gene co-expression network (WGCNA), Bayesian analysis. The apoptosis, necroptosis, efferocytosis FoxO signaling pathways significantly enriched based KEGG enrichment pathway VEGF are expressed, enhancing generation microvessels improving capacity carry oxygen, thus enabling adapt stress. Hypoxia activated glycolysis, enhanced anaerobic metabolism, promoted β-oxidation fatty acids, providing energy maintaining normal eventually antioxidant immune capabilities carp. In summary, this study reveals molecular by which Our findings provide valuable references promoting healthy aquaculture hypoxic-sensitive fish breeding hypoxia-tolerant varieties.

Language: Английский

Citations

1
The laminin receptor is a receptor for Micropterus salmoides rhabdovirus DOI
Sheng Luo, Jiahui Liang, Guan‐Jun Yang

et al.

Journal of Virology, Journal Year: 2024, Volume and Issue: 98(7)

Published: June 25, 2024

ABSTRACT Micropterus salmoides rhabdovirus (MSRV) is an important pathogen of largemouth bass. Despite extensive research, the functional receptors MSRV remained unknown. This study identified host protein, laminin receptor (LamR), as a cellular facilitating entry into cells. Our results demonstrated that LamR directly interacts with G playing pivotal role in attachment and internalization processes MSRV. Knockdown siRNA, blocking cells antibody, or incubating virions soluble protein significantly reduced entry. Notably, we found mediated via clathrin-mediated endocytosis. Additionally, our findings revealed were internalized co-localized early late endosomes. These highlight significance binding target through interaction protein. IMPORTANCE serious epidemic caused by bass, precise mechanism which it invades remains unclear. Here, determined (LamR) novel MSRV, its involved viral internalization, transporting together first report demonstrating life cycle, thus contributing new insights host-pathogen interactions.

Language: Английский

Citations

5
CRISPR/CasRx: A novel antiviral approach to combat largemouth bass (Micropterus salmoides) Rhabdovirus infections DOI

Kechen Yang,

B.M. Ma,

Zhongxin Wu

et al.

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742189 - 742189

Published: Jan. 1, 2025

Language: Английский

Citations

0
Antiviral potential of the arctigenin derivative COA in reducing viral adhesion to the epithelial cell surface against IHNV infection DOI
Yang Hu, Huan Wang, Zixuan Wang

et al.

Fish & Shellfish Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 110278 - 110278

Published: March 1, 2025

Language: Английский

Citations

0
Updates on infectious diseases of largemouth bass: a major review DOI
Shun Yang, Jing Zhao, Nan An

et al.

Fish & Shellfish Immunology, Journal Year: 2024, Volume and Issue: unknown, P. 109976 - 109976

Published: Oct. 1, 2024

Language: Английский

Citations

3
Unlocking the potential: Cinnamomum cassia (L.) D. Don aqueous extracts as natural defenders against MSRV in largemouth bass aquaculture DOI

Guanglu Liu,

Mohan Bu,

Mingyang Qin

et al.

Aquaculture, Journal Year: 2024, Volume and Issue: 595, P. 741606 - 741606

Published: Sept. 11, 2024

Language: Английский

Citations

2
Orange-spotted grouper IFNh response to NNV or MSRV and its potential antiviral activities DOI
Siyou Huang, Rui Zheng,

Yiling Kang

et al.

Fish & Shellfish Immunology, Journal Year: 2023, Volume and Issue: 145, P. 109345 - 109345

Published: Dec. 26, 2023

Language: Английский

Citations

1
Effects of dietary Silphium perfoliatum L. on the growth performance, immunity, intestinal health, and resistance against Nocardia seriolae of largemouth bass (Micropterus salmoides) DOI Creative Commons
Hao Tang, Sheng Luo, Jifeng Chen

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: July 23, 2024

The present study was conducted to evaluate the effect of dietary Silphium perfoliatum L. on growth performance, immunity, intestinal health, and resistance against Nocardia seriolae largemouth bass. Four concentrations S. were prepared: 0% (SPL0), 1% (SPL1), 2.5% (SPL2.5), 5% (SPL5). After 60 d feeding, a challenge test by injecting N. intraperitoneally. results showed that supplementing diets with could promote weight gain rate, specific feed intake. Meantime, also enhance activity immune-related enzymes improve expression genes in liver. Compared control group, improved morphology reduced abundance harmful bacteria microbiota. Importantly, protected bass from infection, resulting relative percent survival (RPS) 24% 31.99% 43.99% In conclusion, boost ability infection.

Language: Английский

Citations

0
Study on the immune enhancers against <i>Micropterus salmoides</i> rhabdovirus infection DOI Creative Commons

Lei Ning,

Chaonan Zhang,

Yanchao Wang

et al.

Journal of Limnology, Journal Year: 2024, Volume and Issue: 83

Published: Oct. 11, 2024

Micropterus salmoides rhabdovirus (MSRV) is one of the most serious pathogens harming M. juvenile, which had brought huge economic losses to farming industry. Studies involving candidate genes clinical diseases, however, are limited. In this study, viral target and manifestation MSRV on juvenile were analyzed, protective effects a single immune enhancer compound evaluated. The results showed that brain, liver, intestine muscle obvious lesions after infection with MSRV. relative expression levels nucleoprotein (N) matrix protein (M) trend increasing at first then decreasing reached peak in each tissue 36 h post-infection. mortality rate was over 90% 7 days infection. enhancers containing free nucleotides Astragalus polysaccharide added diet effectively inhibited replication N M increased survival by 25% 28%. This study provided basic data theoretical reference for analysis pathological mechanism prevention treatment

Language: Английский

Citations

0