ZDHHC3-LYPLA1 regulates PRRSV-2 replication through reversible palmitoylation

Veterinary Microbiology, Journal Year: 2025, Volume and Issue: 301, P. 110368 - 110368

Published: Jan. 5, 2025

Language: Английский

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Enhanced Porcine Reproductive and Respiratory Syndrome Virus Replication in Nsp4- or Nsp2-Overexpressed Marc-145 Cell Lines

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(1), P. 52 - 52

Published: Jan. 13, 2025

Porcine reproductive and respiratory syndrome (PRRS) causes significant economic losses to the swine industry. The killed PRRSV vaccine has been reported be safe could elicit humoral responses. with a high viral antigen load combined robust adjuvants provide good protection against infection. However, virus titer of on successive production cell lines is prerequisite for this strategy. In study, we explored in two recombinant Marc-145 expressing Nsp2 or Nsp4 through lentivirus system. results demonstrated that either cells did not affect morphology growth kinetics but significantly enhanced replication. Overall, our exploration may enable high-yield offer potential tool developing safer more effective vaccines.

Language: Английский

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The role of major and minor structural proteins of porcine reproductive and respiratory syndrome virus in induction of protective immunity

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 19, 2025

Introduction Porcine reproductive and respiratory syndrome virus (PRRSV), an economically significant threat to the world pork production, is notoriously known for its heterogeneity, therefore current vaccines often fail provide efficient cross-protection against diverse PRRSV strains. Methods By making chimeric viruses using HP-PRRSV-2 lineage 8 (JXwn06) 1 NADC30-like strains (CHsx1401) as model organisms, recently results have shown that viral structural protein-coding region critical induction of homologous immunity. In this study, were further constructed by exchanging coding minor (GP2/3/4) or major (GP5/M) proteins JXwn06 on backbone CHsx1401 generate two mutants CHsx1401-GP234 JX CHsx1401-GP5M . Results The subsequent animal experiment showed all three chimeras could confer good protective immunity lethal challenge HP-PRRSV strain JXwn06, survived pigs had much lower lung lesions, faster viremia clearance, tissue load. However, exchange SP a whole performed better than either GP2/3/4 GP5/M alone, in latter groups transient fever following higher load certain tissues, highlighting synergistic role. Interestingly, compared group , excellent comparable group. Discussion Our report revealed important role ORFs2-4 ORFs5-6 regions implications understanding pathogenesis vaccine development.

Language: Английский

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Dynamic interplay between RNA N6-methyladenosine modification and porcine reproductive and respiratory syndrome virus infection

Veterinary Research, Journal Year: 2025, Volume and Issue: 56(1)

Published: March 22, 2025

Abstract N 6 -methyladenosine (m A) has attracted significant attention for its role in regulating the complex interaction between viruses and host cells. Porcine reproductive respiratory syndrome virus (PRRSV) is a pathogen affecting swine health worldwide. Here, we first identified seven m A-enriched peaks PRRSV genomic RNA by A immunoprecipitation sequencing A-seq). Moreover, functional analyses revealed positive correlation modification level replication. Treatment with universal methylation inhibitor 3-deazaadenosine (3-DAA) effectively suppressed replication dose-dependent manner. Furthermore, A-seq was also used to determine landscape of transcriptome PAMs infected pandemic or highly pathogenic strains. Among 4677 transcripts exhibiting altered levels, MAPK14 gene p38/MAPK signalling pathway emerged as preliminary targets A-mediated epigenetic regulation during infection. These findings provide new insights into mechanisms underlying infection may facilitate development anti-PRRSV therapeutics.

Language: Английский

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The commercial PRRSV attenuated vaccine can be a potentially effective live trivalent vaccine vector

Applied Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 109(1)

Published: May 2, 2025

PRRSV is an immunosuppressive virus that prone to secondary infection by other viral and microbial pathogens, exacerbating its harm pigs. The development of multivalent vaccines can prevent control multiple pathogens great significance for the pig industry. In order evaluate potential commercial as vectors trivalent vaccine, this study first embedded mCherry or EGFP genes fluorescent proteins into infectious clone HP-PRRSV MLV vaccine strain GD construct a recombinant strain. Furthermore, we constructed strain, rPRRSV-mCherry-EGFP, expresses both simultaneously evaluated stability immunogenicity exogenous proteins. results showed MARC-145 cells infected with rPRRSV-mCherry-EGFP could express proteins, number positive increased infection, providing more tool options visualization high-throughput screening anti-PRRSV drugs. More importantly, be passaged in at least 10 generations. It induce piglets produce antibodies against PRRSV, EGFP, mCherry, indicating two also good immune responses. Our research suggests using HP-PRRSV-attenuated vector embedding may effective attempt achieve cross-protection three important basis design vaccines. KEY POINTS: • A expressed foreign was constructed. mCherry. had used triple

Language: Английский

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Porcine reproductive and respiratory syndrome virus nsp2-related proteins induce host translational arrest by specifically impairing the mTOR signaling cascade

Veterinary Microbiology, Journal Year: 2025, Volume and Issue: 306, P. 110562 - 110562

Published: May 13, 2025

Language: Английский

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