Bergamottin Inhibits Bovine Viral Diarrhea Virus Replication by Suppressing ROS-Mediated Endoplasmic Reticulum Stress and Apoptosis DOI Creative Commons

Jinhua Yin,

Jialu Zhang,

Yi Liu

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(8), P. 1287 - 1287

Published: Aug. 13, 2024

Bovine viral diarrhea virus (BVDV) is one of the most important etiological agents that causes serious economic losses to global livestock industry. Vaccines usually provide limited efficacy against BVDV due emergence mutant strains. Therefore, developing novel strategies combat infection urgently needed. Bergamottin (Berg), a natural furanocoumarin compound, possesses various pharmaceutical bioactivities, but its effect on remains unknown. The present study aimed investigate antiviral and underlying mechanism Berg infection. results showed exhibited an inhibitory replication in MDBK cells by disrupting release, rather than directly inactivating particles. Mechanistically, inhibits suppressing endoplasmic reticulum (ER) stress-mediated apoptosis via reducing reactive oxygen species (ROS) generation. Studies vivo demonstrated oral gavage at doses 50 mg/kg 75 significantly reduced load within intestines spleen BVDV-challenged mice. Furthermore, histopathological damage oxidative stress caused were also mitigated with treatment. Our data indicated suppressed propagation both vitro vivo, suggesting it as promising option BVDV.

Language: Английский

Phlorizin Limits Bovine Viral Diarrhea Virus Infection in Mice via Regulating Gut Microbiota Composition DOI
Zhicheng Zhao, Chuang Li,

Jiang Huang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(17), P. 9906 - 9914

Published: April 16, 2024

Phlorizin (PHZ) is one of the main pharmacologically active ingredients in

Language: Английский

Citations

4
The long noncoding RNA APR attenuates PPRV infection-induced accumulation of intracellular iron to inhibit membrane lipid peroxidation and viral replication DOI Creative Commons
Bo Wen, Wen‐Chi Chang, Lulu Yang

et al.

mBio, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

ABSTRACT Peste des petits ruminants virus (PPRV) is an important pathogen that has long been a significant threat to small ruminant productivity worldwide. Iron metabolism vital the host and pathogen. However, mechanism underlying host-PPRV interactions from perspective of iron iron-mediated membrane lipid peroxidation not reported thus far. In this study, we identified novel long-noncoding RNA (lncRNA), APR, impairs PPRV infectivity by sponging miR-3955-5p, negative microRNA (miRNA) directly targets gene encoding ferritin-heavy chain 1 (FTH1) protein. Importantly, demonstrated infection causes aberrant cellular accumulation increasing transferrin receptor (TFRC) expression induces reticulophagy ferroptosis, which benefits replication. Moreover, enhanced localization on endoplasmic reticulum (ER) caused ER damage promoting excess induce reticulophagy. Interestingly, APR decreased infection-induced intracellular Fe 2+ via miR-3955-5p/FTH1 axis ultimately inhibited ferroptosis. Additionally, our results indicate interferon regulatory factor promotes transcription positively regulating promoter activity after infection. Taken together, findings revealed new pattern PPRV-host interactions, involving noncoding regulation, metabolism, iron-related peroxidation, critical for understanding defense against pathogenesis PPRV. IMPORTANCE Many viruses have engage in facilitate their replication pathogenesis. interacts with cells or yet reported. Our data provide first direct evidence promote viral reveal lncRNA, as regulator FTH1 protein expression. increased TFRC expression, more importantly, overload well promoted enhancing induced ferroptosis Furthermore, factor, lncRNA was found decrease protein, thereby attenuating inhibiting present study insight into interaction potential therapeutics

Language: Английский

Citations

0
Identification of three novel B cell epitopes targeting the bovine viral diarrhea virus NS3 protein for use in diagnostics and vaccine development DOI
Yuanyuan Zhang, Jing Cheng,

Wenxiao Liu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142767 - 142767

Published: April 1, 2025

Language: Английский

Citations

0
Peste des petits ruminants virus (PPRV) induces ferroptosis via LONP1-mediated mitochondrial GPX4 degradation in cell culture DOI Creative Commons
Zhou Fang, Shirley Cheng, Zhijun Li

et al.

Journal of Virology, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

ABSTRACT Peste des petits ruminants virus (PPRV) is an important pathogen that seriously affects the productivity of small worldwide. Ferroptosis a programmed cell death characterized by iron-dependent lipid peroxidation and accumulation reactive oxygen species (ROS). Emerging evidence has demonstrated mitochondria play diverse roles in process ferroptosis, but interaction between ferroptosis during infection remains largely unknown. Here, we demonstrate PPRV induces including Fe 2+ overload, peroxidation, shrinkage mitochondria. Importantly, crucial role PPRV-induced decreased mitochondrial GPX4 Mechanistically, downregulates Lon protease-1 (LONP1) expression, multifaceted enzyme essential for maintaining homeostasis function, which leads to degradation through Nrf2/Keap pathway ROS More importantly, tightly associated with inflammatory responses enhanced replication. Overall, this study first show LONP1-mediated involved infection. IMPORTANCE transient severe immunosuppression host, threatens both livestock endangered susceptible wildlife populations many countries. Despite extensive research, it unknown whether causes what mechanism regulation is. Our data provide direct relationship (LONP1)-mediated dysfunctional consequent induction pathogenesis. via mitochondria, replication levels. Taken together, our research provided new insight into understanding effect on pathogenesis revealed potential therapeutic target antiviral intervention.

Language: Английский

Citations

0
Bergamottin Inhibits Bovine Viral Diarrhea Virus Replication by Suppressing ROS-Mediated Endoplasmic Reticulum Stress and Apoptosis DOI Creative Commons

Jinhua Yin,

Jialu Zhang,

Yi Liu

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(8), P. 1287 - 1287

Published: Aug. 13, 2024

Bovine viral diarrhea virus (BVDV) is one of the most important etiological agents that causes serious economic losses to global livestock industry. Vaccines usually provide limited efficacy against BVDV due emergence mutant strains. Therefore, developing novel strategies combat infection urgently needed. Bergamottin (Berg), a natural furanocoumarin compound, possesses various pharmaceutical bioactivities, but its effect on remains unknown. The present study aimed investigate antiviral and underlying mechanism Berg infection. results showed exhibited an inhibitory replication in MDBK cells by disrupting release, rather than directly inactivating particles. Mechanistically, inhibits suppressing endoplasmic reticulum (ER) stress-mediated apoptosis via reducing reactive oxygen species (ROS) generation. Studies vivo demonstrated oral gavage at doses 50 mg/kg 75 significantly reduced load within intestines spleen BVDV-challenged mice. Furthermore, histopathological damage oxidative stress caused were also mitigated with treatment. Our data indicated suppressed propagation both vitro vivo, suggesting it as promising option BVDV.

Language: Английский

Citations

0