Human cell adaptation of the swine acute diarrhea syndrome coronavirus spike protein DOI Creative Commons
Rafael Sanjuán, Jérémy Dufloo,

Clàudia Soriano-Tordera

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 2, 2024

Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a recently identified highly pathogenic swine coronavirus. In vitro, SADS-CoV can infect cell lines from many different species, including humans, highlighting its high zoonotic potential. Coronavirus spike glycoproteins play critical role in viral entry and are involved determining host range cellular tropism. Here, we used experimental evolution to investigate how the protein adapts human cells identify potential variants with increased infectivity. We evolved recombinant vesicular stomatitis virus expressing (rVSV-SADS) three lines. After ten passages, replication was observed, mutations were by sequencing. Mutations functionally characterized terms of fitness, processing fusogenicity. Our results thus human-adaptive that may further enhance

Language: Английский

Interplay of swine acute diarrhoea syndrome coronavirus and the host intrinsic and innate immunity DOI Creative Commons
Fei Zhao,

Xiao Cong,

Xiaobo Huang

et al.

Veterinary Research, Journal Year: 2025, Volume and Issue: 56(1)

Published: Jan. 9, 2025

Abstract Swine acute diarrhoea syndrome coronavirus (SADS-CoV), a novel HKU2-related of bat origin, is newly emerged swine enteropathogenic that causes severe in piglets. SADS-CoV has broad cell tropism with the capability to infect wide variety cells from human and diverse animals, which implicates its ability hold high risks cross-species transmission. The intracellular antiviral immunity, comprised intrinsic innate represents first line host defence against viral infection prior onset adaptive immunity. To date, there are no vaccines drugs approved prevent or treat infection. Understanding mutual relationship between immunity crucial for development SADS-CoV. Here, we review recent advancements our understanding interplay extensive in-depth investigation on their interactive will contribute identification new targets developing intervention strategies control

Language: Английский

Citations

0
Swine Acute Diarrhea Syndrome Coronavirus: An Overview of Virus Structure and Virus–Host Interactions DOI Creative Commons

Seung-Hwa Baek,

Jung-Eun Park

Animals, Journal Year: 2025, Volume and Issue: 15(2), P. 149 - 149

Published: Jan. 9, 2025

SADS-CoV, a recently identified Rhinolophus bat coronavirus HKU2-associated swine coronavirus, is malignant pathogen that causes acute diarrhea, severe and weight loss in infected piglets. The virus was first detected Guangdong Province, China, 2017 has since been observed Jiangxi, Fujian, Guangxi Provinces. In 2023, the Henan inland China. This can infect various cell lines, including human showing significant potential for cross-species transmission posing possible zoonotic threat. However, molecular biology of SADS-CoV remains largely unknown, there are no commercially available therapeutics or vaccines to prevent infection. this review, an update on progress research provided, with focus history outbreaks, characteristics virus, its interactions host, developments vaccines.

Language: Английский

Citations

0
Adaptive truncation of the S gene in IBV during chicken embryo passaging plays a crucial role in its attenuation DOI Creative Commons

Rong Liang,

Kangchengyin Liu,

Yingfei Li

et al.

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(7), P. e1012415 - e1012415

Published: July 30, 2024

Like all coronaviruses, infectious bronchitis virus, the causative agent of in chickens, exhibits a high mutation rate. Adaptive mutations that arise during production live attenuated vaccines against IBV often decrease virulence. The specific impact these on viral pathogenicity, however, has not been fully elucidated. In this study, we identified at 3' end S gene an strain was serially passaged chicken embryos, and showed resulted 9-aa truncation cytoplasmic tail (CT) protein. This phenomenon CT previously observed other coronaviruses such as porcine epidemic diarrhea virus. We next discovered protein loss endoplasmic-reticulum-retention signal (KKSV). Rescue experiments with recombinant viruses confirmed deletion KKSV motif impaired localization to endoplasmic-reticulum-Golgi intermediate compartment (ERGIC) increased its expression cell surface. significantly reduced incorporation into particles, early subgenomic RNA synthesis, ultimately invasion efficiency CEK cells. vivo chickens pathogenicity mutant strains. Additionally, adaptive altered TRS-B ORF3 impacted transcriptional regulation gene. Our findings underscore significance attenuation infection provide novel strategy for development vaccines.

Language: Английский

Citations

3
Human cell adaptation of the swine acute diarrhea syndrome coronavirus spike protein DOI Creative Commons
Rafael Sanjuán, Jérémy Dufloo,

Clàudia Soriano-Tordera

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 2, 2024

Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a recently identified highly pathogenic swine coronavirus. In vitro, SADS-CoV can infect cell lines from many different species, including humans, highlighting its high zoonotic potential. Coronavirus spike glycoproteins play critical role in viral entry and are involved determining host range cellular tropism. Here, we used experimental evolution to investigate how the protein adapts human cells identify potential variants with increased infectivity. We evolved recombinant vesicular stomatitis virus expressing (rVSV-SADS) three lines. After ten passages, replication was observed, mutations were by sequencing. Mutations functionally characterized terms of fitness, processing fusogenicity. Our results thus human-adaptive that may further enhance

Language: Английский

Citations

0