The link between ancient microbial fluoride resistance mechanisms and bioengineering organofluorine degradation or synthesis

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 30, 2024

Abstract Fluorinated organic chemicals, such as per- and polyfluorinated alkyl substances (PFAS) fluorinated pesticides, are both broadly useful unusually long-lived. To combat problems related to the accumulation of these compounds, microbial PFAS organofluorine degradation biosynthesis less-fluorinated replacement chemicals under intense study. Both efforts undermined by substantial toxicity fluoride, an anion that powerfully inhibits metabolism. Microorganisms have contended with environmental mineral fluoride over evolutionary time, evolving a suite detoxification mechanisms. In this perspective, we synthesize emerging ideas on defluorination/fluorination resistance mechanisms identify best approaches for bioengineering new degrading making compounds.

Language: Английский

---

Advances in macro-bioactive materials enhancing dentin bonding

Discover Nano, Journal Year: 2025, Volume and Issue: 20(1)

Published: Feb. 17, 2025

The long-term stability of dentin bonding is equally crucial for minimally invasive aesthetic restoration. Although the meets clinical standards at initial stage, its efficacy remains suboptimal owing to impact physiological factors. Herein, we present a comprehensive analysis macro-bioactive materials, including nanomaterials and polymer improve longevity extend lifespan adhesive prosthetics through various mechanisms achieve sustained stable effects over an extended period. On one hand, materials directly inhibit enzymatic activity matrix metalloproteinases (MMPs) or impede acidogenic abilities cariogenic microorganisms, thereby enhancing local pH within oral cavity. other they indirectly prevent activation MMPs, safeguarding structural integrity resin–dentin interface efficiently stability. Moreover, these establish cross-links with collagen fibers, promoting bionic remineralization protecting exposed fibers hybrid layer from degradation. These processes ultimately enhance mechanical properties

Language: Английский

---

Molecules Targeting EriC ^F 1 Increase Streptococcus mutans Fluoride Sensitivity

Journal of Dental Research, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Dental caries, as one of the prevalent oral infectious diseases worldwide, constitutes a considerable disease burden. Fluoride has been widely used to prevent dental caries for decades. However, fluoride alone may not always be sufficient. The major cariogenic bacterial species, Streptococcus mutans , effectively controlled by daily exposure, possibly because it detoxification mechanism. Studies have shown that most microorganisms exporters dedicated exporting ions (F − ). S. possesses 2 homologous genes, eriC F 1 and which encode exporters, but their function fully clarified. In this work, we constructed markerless gene deletion mutants, overexpression, complemented strains UA159. Assessing sensitivity, intracellular levels, cell membrane permeability revealed EriC was functional unit exporter in . To further enhance antibacterial efficiency fluoride, identified 3 diphenylurea derivatives might target molecular docking, significantly enhanced effect sodium (NaF) synergistically impeding efflux, demonstrated chequerboard broth microdilution assays. Moreover, these compounds combined with mM NaF impaired cariogenicity vivo good biocompatibility, especially 9 15. Collectively, findings suggest could serve potential prevention, targeting valuable therapeutic approach when providing promising measures prevention.

Language: Английский

---

Enhancement of Fluoride’s Antibacterial and Antibiofilm Effects against Oral Staphylococcus aureus by the Urea Derivative BPU

Antibiotics, Journal Year: 2024, Volume and Issue: 13(10), P. 930 - 930

Published: Sept. 30, 2024

Background: The oral cavity is an important but often overlooked reservoir for Staphylococcus aureus. effective control and prevention of S. aureus colonization infection in the maxillofacial regions are crucial public health. Fluoride widely used dental care its remineralization antibacterial properties. However, effectiveness against has not been thoroughly investigated. Objectives: This study aimed to evaluate potential combining sodium fluoride (NaF) with compounds enhance antibiofilm effects Method: We found that a urea derivative significantly enhances efficacy by promoting retention ions within cells. synergistic BPU NaF were confirmed through various assays, including checkerboard time-kill growth curve analysis. These findings further supported additional methods, transmission electron microscopy (TEM), silico simulations, gene overexpression studies. Results: suggest targeting ion membrane exporters could efficacy. When combined fluoride, 1,3-Bis [3,5-bis(trifluoromethyl)phenyl]urea (BPU) showed increased inhibiting reducing established biofilms. Conclusions: novel combination represents promising therapeutic strategy treating biofilm-associated infections, offering new healthcare. To fully clinical this therapy, vivo studies essential.

Language: Английский

---

Conserved signaling modules regulate filamentous growth in fungi: a model for eukaryotic cell differentiation

Genetics, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 6, 2024

Abstract Eukaryotic organisms are composed of different cell types with defined shapes and functions. Specific produced by the process differentiation, which is regulated signal transduction pathways. Signaling pathways regulate differentiation sensing cues controlling expression target genes whose products generate specific attributes. In studying how cells differentiate, fungi have proved valuable models because their ease genetic manipulation striking morphologies. Many fungal species undergo filamentous growth—a specialized growth pattern where produce elongated tube-like projections. Filamentous promotes expansion into new environments, including invasion plant animal hosts pathogens. The same signaling that in also control throughout eukaryotes include highly conserved mitogen-activated protein kinase (MAPK) pathways, focus this review. many species, mucin-type sensors MAPK to response diverse stimuli. Once activated, reorganize polarity, induce changes adhesion, promote secretion degradative enzymes mediate access environments. However, pathway regulation complicated related can share components each other yet unique responses (i.e. specificity). addition, function integrated networks regulatory integration). Here, we discuss specificity integration several yeast (mainly Saccharomyces cerevisiae Candida albicans) focusing on filamentation pathway. Because strong evolutionary ties between a deeper understanding established increasingly reveal fundamentally mechanisms underlying eukaryotic differentiation.

Language: Английский

---

A prescription for engineering PFAS biodegradation

Biochemical Journal, Journal Year: 2024, Volume and Issue: 481(23), P. 1757 - 1770

Published: Nov. 25, 2024

Per- and polyfluorinated chemicals (PFAS) are of rising concern due to environmental persistence emerging evidence health risks humans. Environmental is largely attributed a failure microbes degrade PFAS. PFAS recalcitrance has been proposed result from chemistry, specifically C-F bond strength, or biology, negative selection fluoride toxicity. Given natural evolution many hurdles, this review advocates for strategy laboratory engineering evolution. Enzymes identified participate in defluorination reactions have discovered all Enzyme Commission classes, providing palette metabolic engineering. In vivo biodegradation will require multiple types powerful mitigation mechanisms act concert. The necessary steps to: (1) engineer bacteria that survive very high, unnatural levels fluoride, (2) design, evolve, screen enzymes cleave C–F bonds broader array substrates, (3) create overall physiological conditions make positive selective pressure with substrates.

Language: Английский

---