Biomass and Bioenergy, Journal Year: 2025, Volume and Issue: 199, P. 107899 - 107899
Published: April 16, 2025
Language: Английский
Environmental Microbiology, Journal Year: 2025, Volume and Issue: 27(1)
Published: Jan. 1, 2025
ABSTRACT The Hepatincolaceae ( Alphaproteobacteria ) are a group of bacteria that inhabit the gut arthropods and other ecdysozoans, associating extracellularly with microvilli. Previous phylogenetic studies, primarily single‐gene analyses, suggested their relationship to Holosporales , which includes intracellular in protist hosts. However, genomics is still its early stages. In this study, number available genomes was increased examine evolutionary functional characteristics. It found previous grouping incorrect due sequence compositional biases form an independent branch within . This led reinterpretation features, proposing new scenario involves adaptation host association compared distinct specificities. exhibit greater nutritional flexibility, utilising various molecules thriving anaerobic conditions. they have less complex mechanism for modulating interactions, likely direct than those bacteria. addition, representatives show several lineage‐specific traits related differences species life
Language: Английский
Citations
1
Microbiome, Journal Year: 2024, Volume and Issue: 12(1)
Published: Oct. 15, 2024
The microbial landscape within termite guts varies across families. gut microbiota of lower termites (LT) is dominated by cellulolytic flagellates that sequester wood particles in their digestive vacuoles, whereas the flagellate-free higher (HT), activity has been attributed to fiber-associated bacteria. However, little known about role individual lineages fiber digestion, particularly LT.
Language: Английский
Citations
7
Environmental Microbiology, Journal Year: 2025, Volume and Issue: 27(1)
Published: Jan. 1, 2025
ABSTRACT Cellulolytic flagellates are essential for the symbiotic digestion of lignocellulose in gut lower termites. Most species associated with host‐specific consortia bacterial symbionts from various phyla. 16S rRNA‐based diversity studies and taxon‐specific fluorescence situ hybridization revealed a termite‐specific clade Actinomycetales that colonise cytoplasm Trichonympha spp. other flagellates, representing only known case intracellular Actinomycetota protists. Comparative analysis eleven metagenome‐assembled genomes termites allowed us to describe them as new genera Bifidobacteriaceae . Like previously investigated Candidatus Ancillula trichonymphae, they ferment sugars via bifidobacterium shunt but, unlike their free‐living relatives, experienced significant genome erosion. Additionally, acquired functions by horizontal gene transfer bacteria, including capacity produce hydrogen. Members genus (average size 1.56 ± 0.2 Mbp) retained most pathways synthesis amino acids, threonine/serine exporter, providing concrete evidence basis mutualistic relationship host. By contrast, Opitulatrix (1.23 0.1 lost biosynthetic capacities, indicating an originally symbiosis is on decline.
Language: Английский
Citations
0
Microbial Ecology, Journal Year: 2025, Volume and Issue: 88(1)
Published: March 14, 2025
Abstract The order Holosporales is a broad and ancient lineage of bacteria obligatorily associated with eukaryotic hosts, mostly protists. Significantly, this similar to other evolutionary distinct bacterial lineages (e.g. Rickettsiales Chlamydiae ). Here, we provide detailed comprehensive account on the current knowledge . First, acknowledging up-to-date phylogenetic reconstructions recent nomenclatural proposals, reevaluate their taxonomy, thus re-ranking them as suborder, i.e. Holosporineae , within Rhodospirillales Then, examine diversity presenting 20 described genera many yet undescribed sub-lineages, well variety respective environments provenance which belong several different supergroups. Noteworthy representatives are infectious intranuclear Holospora host manipulator ‘ Caedimonas ’, farmed shrimp pathogen Candidatus Hepatobacter’. Next, put these in context whole by comparing available data least studied representatives, including genome sequences. Accordingly, reason most probable trajectories for interactions, specificity, emergence potential pathogens aquaculture possibly humans, future research directions investigate those open points
Language: Английский
Citations
0
Insects, Journal Year: 2025, Volume and Issue: 16(3), P. 325 - 325
Published: March 19, 2025
Termites are eusocial insects with functionally specialized workers and soldiers, both sharing the same genotype. Additionally, lower termites host flagellates in their hindguts that assist wood digestion. However, worker-biased soldier-biased gene expression patterns of host–flagellate symbiotic system remain underexplored most taxonomic groups. In this study, we sequenced high-depth transcriptomes from soldiers a termite, Neotermes binovatus (Kalotermitidae), to investigate differentially expressed termite transcripts, flagellate transcript abundance, co-expression pairs castes. The transcripts were enriched functions related cuticle development, nervous regulation, pheromone biosynthesis, metabolism, whereas predominantly involved muscle development kinesis, body morphogenesis, protein modification, aggression. Flagellate orders Cristamonadida, Trichomonadida, Tritrichomonadida, Oxymonadida identified abundance tending be higher than soldiers. Furthermore, observed much larger number strong correlations between suggesting possibility depend more on food processed by worker holobionts own system. This research provides insights into functional specialization soldier castes termites, supporting workers’ roles nest maintenance, preliminary processing, communication, while emphasizing defensive role it offers new perspectives potential termite-flagellate interactions underscores need for whole-genome data further studies.
Language: Английский
Citations
0
Systematic and Applied Microbiology, Journal Year: 2025, Volume and Issue: 48(3), P. 126606 - 126606
Published: April 13, 2025
The tree of life comprises many deep-branching lineages with no or only very few cultured representatives. One such lineage is the phylum Elusimicrobiota, which contains two described species and whose biology has been poorly explored. We isolated three new from this intestinal tracts cockroaches. Like their closest relative, Elusimicrobium minutum, member family Elusimicrobiaceae to date, they are small, pleomorphic gram-negative rods characterized by a distinct cell cycle, like all ultramicrobacteria, pass through 0.22-μm filter membrane. Physiological characterization isolates revealed that obligately anaerobic fermenters lack catalase cytochrome c oxidase activities but can remove oxygen environment in non-respiratory manner. Their substrate range limited hexoses, as d-glucose, d-galactose, N-acetyl-d-glucosamine, fermented lactate, acetate, ethanol, hydrogen major products. Comparative genome analysis, included more than 100 MAGs uncultured Elusimicrobiaceae, underlying metabolic pathways outlined phylogenomic framework family. Based on phylogenomic, physiological, morphological evidence, we describe Parelusimicrobium proximum gen. nov., sp. posterum simillimum nov. under rules ICNP. high-quality genomes representatives, propose comprehensive taxonomy SeqCode, including genera Avelusimicrobium, Proelusimicrobium, candidate genus "Pseudelusimicrobium".
Language: Английский
Citations
0
Biomass and Bioenergy, Journal Year: 2025, Volume and Issue: 199, P. 107899 - 107899
Published: April 16, 2025
Language: Английский
Citations
0