ACS ES&T Water,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 5, 2024
Free-living
amoebae
represent
a
reservoir
for
some
environmental
bacteria
that
are
opportunistic
human
pathogens.
Legionella
pneumophila
is
Gram-negative,
amoeba-resistant
bacterium,
which
upon
inhalation
can
cause
life-threatening
atypical
pneumonia
termed
Legionnaires'
disease.
The
capacity
to
grow
within
and
other
protozoa
prerequisite
L.
virulence
requires
the
bacterial
Icm/Dot
type
IV
secretion
system
(T4SS).
plate
test
(APT)
exploits
of
virulent
rather
than
Icm/Dot-deficient
on
BCYE
agar
plates
in
presence
amoeba.
Here,
we
apply
APT
different
species
optimize
by
varying
amoeba
concentration,
growth
temperature,
culture
media,
additives
such
as
fungicides.
optimized
enriches
suppresses
microbial
flora.
Accordingly,
allows
identification
complex
water
samples
further
pathogenic
Delftia
acidovorans,
rare
causative
agent
sepsis
pneumonia.
The Science of The Total Environment,
Journal Year:
2025,
Volume and Issue:
975, P. 179204 - 179204
Published: April 7, 2025
Free-living
amoebae
(FLA)
are
ubiquitous
protists
found
in
water,
feeding
mainly
on
bacteria.
While
most
FLA
harmless,
Acanthamoeba
spp.
and
Naegleria
fowleri
can
cause
keratitis
and/or
meningitis.
host
amoeba-resistant
bacteria
(ARB),
but
their
natural
bacterial
microbiota
is
largely
unknown.
This
study
aimed
to
identify
the
of
fowleri,
castellanii,
lenticulata,
sp.
T17,
isolated
from
untreated
(rivers)
treated
(tap)
waters
Guadeloupe.
The
whole
water
source
grown
with
E.
coli
under
axenic
culture
conditions,
during
successive
passages,
were
characterized
using
16S
rRNA
gene
metabarcoding.
culturable
subset
ARB
was
identified
by
mass
spectrometry
(MALDI-TOF
MS)
followed
conventional
PCR,
antibiotic
resistance
analyzed
disk
diffusion
method.
Transmission
electron
microscopy
used
locate
within
amoebae.
metabarcoding
analyses
Salmonella,
Enterobacter
Klebsiella
genera
as
abundant
waters.
However,
frequently
detected
(ARB)
Bosea,
Escherichia-Shigella,
Microbacterium,
Pseudomonas
genera.
Our
findings
revealed,
for
first
time,
occurrence
several
N.
including
Escherichia
coli.
Additionally,
we
Legionella
A.
castellanii
Bordetella
lenticulata.
four
pathogenic
showed
both
temporary
permanent
associations
various
genera,
depending
number
passages
conditions.
species
distinct
exhibited
different
antibiotics.
cytoplasm
trophozoites.
presence
Guadeloupe's
drinking
systems
pose
health
risks.
results
highlight
need
regular
monitoring
ensure
safety
understanding
amoebae-bacteria
interactions
better
management.
also
questions
immune
response
amoeba
infection.
Current Research in Microbial Sciences,
Journal Year:
2025,
Volume and Issue:
8, P. 100338 - 100338
Published: Jan. 1, 2025
Legionella
pneumophila,
the
causative
agent
of
Legionnaires'
disease,
interacts
in
environment
with
free-living
amoebae
that
serve
as
replicative
niches
for
bacteria.
Among
these
amoebae,
Acanthamoeba
castellanii
is
a
natural
host
water
networks
and
model
commonly
used
to
study
interaction
between
L.
pneumophila
its
host.
However,
certain
crucial
aspects
this
remain
unclear.
One
such
aspect
role
oxidative
stress,
studies
focusing
on
reactive
oxygen
species
(ROS)
production
by
putting
less
emphasis
involvement
host's
antioxidant
defenses
during
infectious
process.
In
study,
we
propose
examine
consequences
infection
wild-type
or
an
isogenic
ΔdotA
mutant
strain,
which
unable
replicate
intracellularly,
A.
castellanii.
For
purpose,
looked
at
ROS
levels,
defense
transcripts,
metabolites
linked
amoeba's
defenses.
It
known
WT
can
block
activation
NADPH
oxidase
soon
it
enters
macrophage
suppress
compared
strain.
addition,
has
been
shown
macrophages
decreases
24
h
p.i.;
here
confirm
result
suggest
decrease
could
be
partly
explained
differentially
regulated
transcripts
6
p.i..
We
also
explored
metabolome
infected
not
pneumophila.
617
identified,
four
reduced
abundances
may
involved
responses.
This
suggests
hijack
replication
maintain
level
ROS.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 16, 2025
Sexual
reproduction
in
basidiomycete
fungi
is
governed
by
MAT
loci
(P/R
and
HD),
which
exhibit
remarkable
evolutionary
plasticity,
characterized
expansions,
rearrangements,
gene
losses
often
associated
with
mating
system
transitions.
The
sister
genera
Cryptococcus
Kwoniella
provide
a
powerful
framework
for
studying
evolution
owing
to
their
diverse
reproductive
strategies
distinct
architectures,
spanning
bipolar
tetrapolar
systems
either
linked
or
unlinked
loci.
Building
on
recent
large-scale
comparative
genomic
analyses,
we
generated
additional
chromosome-level
assemblies
uncovering
trajectories
shaping
organization.
Contrasting
the
small-scale
expansions
acquisitions
observed
Kwoniella,
our
analyses
revealed
independent
of
P/R
locus
Cryptococcus,
possibly
driven
pheromone
duplications.
Notably,
these
coincided
an
enrichment
AT-rich
codons
pronounced
GC-content
reduction,
likely
recombination
suppression
relaxed
codon
usage
selection.
Diverse
modes
linkage
were
also
identified,
including
three
previously
unrecognized
transitions:
one
resulting
pseudobipolar
arrangement
two
leading
bipolarity.
All
transitions
involved
translocations.
In
configuration,
HD
remained
same
chromosome
but
genetically
unlinked,
whereas
additionally
featured
rearrangements
that
fused
into
nonrecombining
region.
Mating
assays
confirmed
sexual
cycle
C.
decagattii,
demonstrating
its
ability
undergo
sporulation.
Progeny
analysis
K.
mangrovensis
substantial
ploidy
variation
aneuploidy,
stemming
from
haploid-diploid
mating,
yet
evidence
loss
heterozygosity
indicates
meiotic
exchange
occurs
despite
irregular
segregation.
Our
findings
underscore
importance
continued
diversity
sampling
provides
further
convergent
basidiomycetes,
offering
new
insights
genetic
chromosomal
changes
driving
ЗДОРОВЬЕ НАСЕЛЕНИЯ И СРЕДА ОБИТАНИЯ - ЗНиСО / PUBLIC HEALTH AND LIFE ENVIRONMENT,
Journal Year:
2025,
Volume and Issue:
unknown, P. 57 - 65
Published: March 1, 2025
Introduction:
The
relevance
of
the
methodological
development
subsystem
sanitary
and
environmental
protistology
in
conditions
high
anthropogenic
pressure
on
environment
is
associated
with
need
to
monitor
safety
cenotic
changes
natural
ecosystems
order
assess
their
impact
public
health.
Objective:
To
analyze
up-to-date
areas
modern
scientific
research
unicellular
organisms
human
biota.
Materials
methods:
article
presents
a
literature
review
findings
published
1990–2022
found
PubMed,
Google
Scholar,
eLibrary,
CyberLeninka,
Scopus,
disserCat
information
portals
platforms.
topic
was
searched
for
using
following
keywords:
protists,
protistology,
medical
genetic
morphological
diversity
organisms,
role
protozoa
nature,
exemplary
models.
initial
selection
consisted
more
than
60
publications;
40
45
initially
identified
articles
were
selected
analysis.
Description
infrastructural
components
biota
internal
organizational
inclusion
criterion
while
that
mechanisms
interaction
biomedical
processes
exclusion
one.
Drinking
water
opportunistic
pathogens
(OPs)
and
disinfection
byproducts
(DBPs)
both
pose
risks
to
public
health,
their
variable
occurrence
from
source
tap
complicates
efforts
control
them
simultaneously.
Management
of
OPs
DBPs
is
further
hindered
by
the
historical
division
between
microbial
chemical
research.
This
review
brings
together
current
knowledge
regarding
DBPs,
identifies
factors
that
influence
both,
highlights
areas
where
research
needed
better
understand
health
risks.
First,
we
examine
understanding
how
are
jointly
influenced
physicochemical
parameters,
characteristics,
treatment
processes
including
disinfection,
distribution
system
properties.
Temperature,
for
example,
can
affect
OP
DBP
occurrence,
higher
temperatures
promote
growth
some
OPs,
such
as
Legionella
pneumophila,
but
temperature's
effect
on
species-dependent.
Methods
quantifying
associated
with
(quantitative
risk
assessment)
(chemical
compared,
finding
numerous
assumptions
data
gaps
each
method
limit
comparability
across
contaminant
types.
We
highlight
urgent
need
fill
existing
develop
a
more
unified
framework
so
move
toward
holistic
assessment
provides
suggestions
future
research,
highlighting
ways
researchers
might
utilize
established
practices
in
or
studies
our
other.
For
analysis
organic
matter
composition,
which
has
advanced
formation,
could
be
utilized
elucidate
characteristics
OPs.
bridges
gap
disciplines,
arguing
collaboration
two
address
pressing
challenges
facing
systems
today.
Pathogens,
Journal Year:
2025,
Volume and Issue:
14(6), P. 546 - 546
Published: May 31, 2025
Acanthamoeba,
a
free-living
amoeba
ubiquitous
in
environmental
water,
has
been
considered
as
the
reservoir
of
certain
bacterial
pathogens,
including
Campylobacter
jejuni,
an
intracellular
human
pathogen
causing
self-limiting
gastroenteritis.
Acanthamoeba-C.
jejuni
interaction
mechanisms
may
help
clarify
how
otherwise
fastidious
bacterium
C.
survives
waters.
In
this
study,
we
constructed
single
deletion
mutants
strain
81–176
for
10
selected
genes
(motAB,
ciaB,
kpsE,
virB11,
cheY,
flaAB,
cstII,
docB,
sodB,
and
cadF)
previously
shown
to
be
important
(invasion
survival)
with
mammalian
hosts.
We
used
modified
gentamicin
protection
assay
quantify
internalization
survival
these
wild
type
two
species
Acanthamoeba
(A.
castellanii
A.
polyphaga).
Both
were
significantly
lower
all
compared
both
strains,
except
ΔcstII
(p
<
0.05).
The
results
study
highlight
that
by
interact
hosts
are
conserved
its
interactions
This
understanding
useful
developing
effective
strategies
reduce
transmission
chickens
through
drinking
water.
Current Opinion in Infectious Diseases,
Journal Year:
2025,
Volume and Issue:
unknown
Published: June 5, 2025
Purpose
of
review
This
examines
the
interplay
between
biological
and
anthropogenic
factors
in
development
persistence
antimicrobial
resistance
(AMR)
within
building
plumbing
systems,
which
is
particular
concern
high
risk
setting
such
as
healthcare
facilities.
The
highlights
role
biofilms
amoeba
reservoirs
for
AMR
explores
how
engineering
design
decisions,
governance
structures,
cleaning
protocols
influence
microbial
dynamics.
Recent
findings
Biofilms
provide
a
protective
environment
that
facilitates
horizontal
gene
transfer
enhances
bacterial
to
disinfection.
Amoeba-hosted
bacteria
can
evade
standard
practices,
further
promoting
persistence.
Emerging
technologies,
digital
twin
modelling,
offer
new
opportunities
optimize
mitigation
strategies.
However,
more
consideration
needed
be
given
or
management
decision
may
have
unintended
consequences,
outcomes,
increased
biofilm
growth
from
tap
mixers
low-flow
fixtures,
ineffective
protocols,
inadvertently
worsen
AMR.
Summary
Effectively
managing
systems
requires
multidisciplinary
approach
integrates
microbiology,
engineering,
policy.
Data
driven
assessments
identify
high-risk
areas
require
changes
but
also
enable
targeted
strategies,
reducing
reliance
on
widespread
disinfection
drive
resistance.
Future
policies
must
consider
system-wide
implications
prevent
consequences.
By
addressing
both
drivers,
we
develop
sustainable
solutions
mitigate
risks
beyond.
Frontiers in Cellular and Infection Microbiology,
Journal Year:
2024,
Volume and Issue:
14
Published: Dec. 18, 2024
Amoebae,
fascinatingly
diverse
protists,
showcase
a
dual
nature
that
positions
them
as
both
friends
and
foes
in
our
world.
These
organisms,
defined
by
their
distinctive
pseudopodia,
span
spectrum
from
harmful
to
helpful.
On
the
darker
side,
species
like