Galectin-3 regulates erythropoiesis and enhances the immunoregulatory properties of CD71+ erythroid cells across developmental stages DOI Creative Commons
Shokrollah Elahi,

Zahra Elahi,

Najmeh Bozorgmehr

et al.

The Journal of Immunology, Journal Year: 2025, Volume and Issue: unknown

Published: March 22, 2025

Abstract Galectins are expressed by different immune and nonimmune cells with diverse immunomodulatory properties. However, their roles in erythropoiesis remain unknown. We investigated the expression of galectin genes splenic CD71+ erythroid (CECs) from neonatal BALB/c mice at various developmental stages using bulk RNA sequencing. Our analysis revealed distinct gene profiles ages. Specifically, CECs day-3 had a markedly pattern compared to those days 6, 12, 28. Notably, Lgals1, Lgals3, Lgals4, Lgals8, Lgals9 were constitutively CECs, galectin-3 (Gal-3) showing predominant surface expression, unlike Gal-1 Gal-9. Further that Gal-3+ exhibited elevated levels TGF-β, ROS, arginase I, VISTA, PD-L1, correlating enhanced immunosuppressive functions. These also demonstrated increased CD45, c-kit, Ki67, p21 levels, indicating heightened proliferative activity despite apoptosis. Moreover, we found displayed activation signaling pathways, including STAT5, MAPK, LCK. Additionally, co-expressed Fas FasL, implicating these molecules regulation early erythroblasts. Gal-3 interacted CD71 GARP, influencing CECs’ immunoregulatory roles. In tissue-specific studies, varying frequencies across spleen, liver, bone marrow (BM), notable variations placenta fetal liver. results paralleled human BM–derived which high levels. findings emphasize critical role modulating suggest possess capacities.

Language: Английский

Galectin-3 regulates erythropoiesis and enhances the immunoregulatory properties of CD71+ erythroid cells across developmental stages DOI Creative Commons
Shokrollah Elahi,

Zahra Elahi,

Najmeh Bozorgmehr

et al.

The Journal of Immunology, Journal Year: 2025, Volume and Issue: unknown

Published: March 22, 2025

Abstract Galectins are expressed by different immune and nonimmune cells with diverse immunomodulatory properties. However, their roles in erythropoiesis remain unknown. We investigated the expression of galectin genes splenic CD71+ erythroid (CECs) from neonatal BALB/c mice at various developmental stages using bulk RNA sequencing. Our analysis revealed distinct gene profiles ages. Specifically, CECs day-3 had a markedly pattern compared to those days 6, 12, 28. Notably, Lgals1, Lgals3, Lgals4, Lgals8, Lgals9 were constitutively CECs, galectin-3 (Gal-3) showing predominant surface expression, unlike Gal-1 Gal-9. Further that Gal-3+ exhibited elevated levels TGF-β, ROS, arginase I, VISTA, PD-L1, correlating enhanced immunosuppressive functions. These also demonstrated increased CD45, c-kit, Ki67, p21 levels, indicating heightened proliferative activity despite apoptosis. Moreover, we found displayed activation signaling pathways, including STAT5, MAPK, LCK. Additionally, co-expressed Fas FasL, implicating these molecules regulation early erythroblasts. Gal-3 interacted CD71 GARP, influencing CECs’ immunoregulatory roles. In tissue-specific studies, varying frequencies across spleen, liver, bone marrow (BM), notable variations placenta fetal liver. results paralleled human BM–derived which high levels. findings emphasize critical role modulating suggest possess capacities.

Language: Английский

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