Reference Whole Genome Sequence Analyses and Characterization of a Novel Carnobacterium maltaromaticum Distinct Sequence Type Isolated from a North American Gray Wolf (Canis lupus) Gastrointestinal Tract

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(5), P. 410 - 410

Published: April 27, 2025

We hypothesize that bacteria isolated from free-ranging animals could potentially be useful for practical applications. To meet this objective a Gram-positive bacterium was the gastrointestinal (GI) tract of Gray Wolf (Canis lupus) using Brucella broth with hemin and vitamin K (BBHK). By small ribosomal RNA (16S) gene sequencing initially identified as novel Carnobacterium maltaromaticum strain. The propagated both anaerobically aerobically catalase/oxidase negative by starch hydrolysis well lipase assays. reference whole genome sequence (WGS) obtained Illumina Nanopore sequencing. assembly 3,512,202 bp in length, encoding core bacterial genes GC% content 34.48. No lysogenic bacteriophage were detected, although harbors expression bacteriocin other secondary metabolites potential antimicrobial properties. Multilocus typing (MLST), WGS phylogenetics, average nucleotide identity (ANI), single polymorphism (SNP) analyses isolate’s indicate is newly type (ST). Members Carnobacteria have anti-listeria activities, highlighting their functional Consequently, isolate probiotic canids first report on an axenic C. culture genus Canis.

Language: Английский

Comparative Genome Analysis of Canine Frederiksenia canicola Isolates

Antibiotics, Journal Year: 2024, Volume and Issue: 13(12), P. 1235 - 1235

Published: Dec. 22, 2024

Background/Objectives: The One Health approach is crucial for managing and controlling the spread of antimicrobial resistance. Frederiksenia canicola a recently identified bacterial species that seems to be component oral microbiota dogs; however, its pathogenic nature questionable. Methods: In this study, antibacterial susceptibility F. isolates was determined using disk diffusion broth microdilution methods. Genome-wide comparative analyses were performed identify genetic factors driving virulence drug resistance (e.g., factors, genes (ARGs) prophage-related sequences). Results: Most lacked virulence-associated genes. likely resistant clindamycin, lincomycin neomycin, but susceptible penicillin, erythromycin enrofloxacin. Antimicrobial not found in genomes, sequences identified, suggesting potential transfer associated with between bacteria microbiome. Conclusions: presumably commensal organism low potential, as evidenced by absence As can colonize wide range hosts, including humans, further investigation greater number needed better understand role disease development

Language: Английский