BIOCHEMICAL AND GENETIC MECHANISMS UNDERPINNING ESCHERICHIA COLI CELL CYCLE AND GROWTH TRANSITIONS DOI Creative Commons
Josiah J. Morrison

Published: Jan. 1, 2023

Bacterial cell division and the decision to divide is a process that requires wide variety of factors. In bacterial cells, such as widely studied Escherichia coli, large protein complex, termed Z-ring, assembles at midcell enable division. The Z-ring subject many regulators which govern spatiotemporal positioning ring. made up eukaryotic tubulin homolog FtsZ, polymerizes recruit proteins midcell. FtsZ polymers are tethered cytoplasmic membrane by actin FtsA. Together, FtsA act first step in pathway least 30 septum, 12 essential, constrict one into two identical progeny cells. natural environment, cells not continually dividing they do under laboratory conditions; encounter conditions stress nutrient deprivation, pH fluctuation, oxygen limitation, among others. Some can enter non-replicative, or dormant state, provides them with survival advantages face environmental stresses antibiotic treatment.

Language: Английский

Xylooligosaccharide interferes with the cell cycle and reduces the antibiotic tolerance of avian pathogenic Escherichia coli by associating with its potential anti-metabolic actions DOI Creative Commons
Lulu Ren, Hui Ye,

Jiarong Fang

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 103(12), P. 104405 - 104405

Published: Oct. 10, 2024

This study aimed to probe if xylooligosaccharide (XOS) could act as an antimetabolite impact the cell cycle and antibiotic tolerance of avian pathogenic Escherichia coli (APEC). We firstly measured bacteriostasis XOS against APEC O78 its effect on growth growing different medium. Afterwards, effects xylose operon activation along with were analyzed. The results showed that caused no inhibitory circle did not affect (P > 0.05) LB Besides, was unable grow M9 medium (carbon-free) added XOS. However, exerted a similar role in increasing < expression certain genes including isomerase (XylA)-encoding gene (xylA) xylose-binding periplasmic protein (XylF)-encoding (xylF) O78. molecular docking simulation revealed major monomer components (xylobiose, xylotriose xylotetraose) had stable binding potentials both XylA XylF proteins E. coli, supported by low free energy formation considerable hydrogen bonds between them. subsequent analysis altered cycle-related expression, especially elevated nrdB decreased ihfB degree. Moreover, played 2-deoxy-glucose (a glucose analogue serving typical antimetabolite) lowering number ampicillin-tolerant Collectively, direct be metabolized/utilized it might become then activate transport- metabolism-related O78, thus functioning potential exerting antimetabolic actions. at least partially interpret observed roles interfering diminishing above findings expand knowledges about functions provide basis for exploring novel strategies reduce APEC.

Language: Английский

Citations

2
Bacterial survivors: evaluating the mechanisms of antibiotic persistence DOI
Xiaoyi Shi, Ashraf Zarkan

Microbiology, Journal Year: 2022, Volume and Issue: 168(12)

Published: Dec. 6, 2022

Bacteria withstand antibiotic onslaughts by employing a variety of strategies, one which is persistence. Persistence occurs in bacterial population where subpopulation cells (persisters) survives treatment and can regrow drug-free environment. Persisters may cause the recalcitrance infectious diseases be stepping stone to resistance, so understanding persistence mechanisms critical for therapeutic applications. However, current pervaded paradoxes that stymie research progress, many aspects this cellular state remain elusive. In review, we summarize putative persister mechanisms, including toxin-antitoxin modules, quorum sensing, indole signalling epigenetics, as well reasons behind inconsistent body evidence. We highlight present limitations field underscore clinical context frequently neglected, hope supporting future researchers examining clinically important mechanisms.

Language: Английский

Citations

9
The role of integration host factor in biofilm and virulence of high-alcohol-producing Klebsiella pneumoniae DOI Creative Commons
Zheng Fan,

Tongtong Fu,

Zhoufei Li

et al.

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(5)

Published: Sept. 21, 2023

Klebsiella pneumoniae is a well-known human nosocomial pathogen with an arsenal of virulence factors, including capsular polysaccharides (CPS), fimbriae, flagella, and lipopolysaccharides (LPS). Our previous study found that alcohol acted as essential factor for high-alcohol-producing K. (HiAlc Kpn). Integration host (IHF) nucleoid-associated protein functions global regulator in Escherichia coli. However, the regulatory role IHF remains unknown. In present study, we deletion ihfA or ihfB resulted slight defect bacterial growth, severe absence biofilm formation cytotoxicity, significant reduction production. RNA sequencing differential gene expression analysis showed compared wild-type control, many genes was downregulated ΔihfA ΔihfB strains, such those related to CPS (rcsA, galF, wzi, iscR), LPS (rfbABCD), type I III fimbriae (fim mrk operon), cellulose (bcs iron transporter (feoABC, fhuA, fhuF, tonB, exbB, exbD), quorum sensing (lsr operon sdiA), II secretion system (T2SS) VI (T6SS) (tssG, hcp, gspE). Of these CPS, LPS, are involved formation. addition, could affect production by regulating glucose intake (ptsG), pyruvate formate-lyase, dehydrogenase, tricarboxylic acid (TCA) cycle. data provided new insights into importance HiAlc Kpn. IMPORTANCE causes various infectious diseases, urinary tract infections, hospital-acquired pneumonia, bacteremia, liver abscesses. studies demonstrated Kpn mediated development nonalcoholic fatty disease producing excess endogenous vivo. regulators metabolism, formation, remain unclear. this positively regulate factors cellulose, transporter, AI-2 sensing, T2SS, T6SS Furthermore, regulated results suggested be potential drug target treating diseases caused pneumoniae. Hence, regulation different requires further investigation.

Language: Английский

Citations

5
Molecular mechanism and application of emerging technologies in study of bacterial persisters DOI Creative Commons
Shuo Yuan, Yamin Shen,

Yingying Quan

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 16, 2024

Abstract Since the discovery of antibiotics, they have served as a potent weapon against bacterial infections; however, natural evolution has allowed bacteria to adapt and develop coping mechanisms, ultimately leading concerning escalation multidrug resistance. Bacterial persisters are subpopulation that can survive briefly under high concentrations antibiotic treatment resume growth after lethal stress. Importantly, thought be significant cause ineffective therapy recurrent infections in clinical practice contribute development Therefore, it is essential elucidate molecular mechanisms persister formation precise medical strategies combat persistent infections. However, there many difficulties studying due their small proportion microbiota non-heritable nature. In this review, we discuss similarities differences resistance, tolerance, persistence, viable but non-culturable cells, summarize affect persisters, outline emerging technologies study persisters.

Language: Английский

Citations

1
Deciphering Antibiotic-Targeted Metabolic Pathways in Acinetobacter baumannii: Insights from Transcriptomics and Genome-Scale Metabolic Modeling DOI Creative Commons
Fatma Zehra Sarı, Tunahan Çakır

Life, Journal Year: 2024, Volume and Issue: 14(9), P. 1102 - 1102

Published: Sept. 2, 2024

In the ongoing battle against antibiotic-resistant infections, Acinetobacter baumannii has emerged as a critical pathogen in healthcare settings. To understand its response to antibiotic-induced stress, we integrated transcriptomic data from various antibiotics (amikacin sulfate, ciprofloxacin, polymyxin-B, and meropenem) with metabolic modeling techniques. Key pathways, including arginine proline metabolism, glycine–serine threonine glyoxylate dicarboxylate propanoate were significantly impacted by all four across multiple strains. Specifically, biotin metabolism was consistently down-regulated under polymyxin-B treatment, while fatty acid perturbed amikacin sulfate. Ciprofloxacin induced up-regulation glycerophospholipid metabolism. Validation an independent dataset focusing on colistin treatment confirmed alterations degradation, elongation, By harmonizing genetic metabolite-centric approach, our findings offer insights into intricate adaptations of A. antibiotic pressure, suggesting more effective strategies combat infections.

Language: Английский

Citations

0
Isolation and Identification of Oral Bacteria and Characterization for biofilm formation and antibiotics resistance DOI Creative Commons

FARAH ALI

Wasit Journal of Pure sciences, Journal Year: 2024, Volume and Issue: 3(1), P. 106 - 115

Published: March 30, 2024

In this study, 100 bacterial isolates were obtained from tooth surface swabs of male and female patients with different ages who visited specialized centers dental clinics in Baghdad city after they clinically diagnosed oral (dental) infections by specialist doctors for the period 1/10/2022 to 1/3/2023. The methods employed identify Vitek system, as well highest isolated rate was among Streptococcus mutans species (48%). Isolates S. their drug sensitivity tested against 10 antibiotics using disc diffusion process, showing that separators’ Antibiotic resistance larger than these antibiotics. erythromycin (98.21 94.64%), amoxicillin (85.71)% each them, ampicillin (80.35 83.92)%, Nalidixic acid 87.50)%, cefotaxime (69.64 75.00)%, chloramphenicol 89%), amoxicillin–Clavulanic (58.92 76.78)%), gentamycin (57.14 78.57)%, tetracycline (55.35 66.07)% respectively, while it showed high amikacin (25.00 48.21)% trimethoprim sulfamethaxyl (7.14 19.64)% them respectively.

Language: Английский

Citations

0
CRP and IHF act as host regulators in Royal Jelly's antibacterial activity DOI Creative Commons
Zhenyu Xia,

Yunchang Li,

Jinhao Liu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 21, 2024

Abstract Royal Jelly (RJ) is a natural substance produced by honeybees, serving not only as nutrition for bee brood and queens but also functional food due to its health-promoting properties. Despite well-known broad-spectrum antibacterial activity, the precise molecular mechanism underlying action has remained elusive. In this study, we investigated impact of RJ on bacteria model MG1655 at half maximal inhibitory concentration, employing LC-MS/MS analyze proteomic changes. The differentially expressed proteins were found primarily contribute suppressing gene expression processes, specifically transcription translation, disrupting energy metabolism, inducing oxidative stress. Notably, treatment led marked inhibition superoxide dismutase catalase activities, resulting in heightened damage lipid peroxidation. Furthermore, through protein-protein interaction network analysis using STRING database, identified CRP IHF crucial host regulators responsive RJ. These play pivotal role essential hub genes associated with production antioxidant capabilities. Our findings significantly understanding RJ's mechanism, highlighting potential alternative conventional antibiotics.

Language: Английский

Citations

0
Metabolic flux regulates growth transitions and antibiotic tolerance in uropathogenicEscherichia coli DOI Creative Commons
Josiah J. Morrison,

Daniel A. Banas,

Ellen K. Madden

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 10, 2023

Abstract Reducing growth and limiting metabolism are strategies that allow bacteria to survive exposure environmental stress antibiotics. During infection, uropathogenic Escherichia coli (UPEC) may enter a quiescent state enables them reemerge after completion of successful antibiotic treatment. Many clinical isolates, including the well characterized UPEC strain CFT073, also metabolite-dependent, in vitro is reversible with cues, peptidoglycan-derived peptides amino acids. Here, we show tolerant demonstrate metabolic flux tricarboxylic acid (TCA) cycle regulates via succinyl-CoA. We transcriptional regulator complex IHF FtsZ-interacting protein ZapE, which important for E. division during stress, essential state. Notably, addition engaging FtsZ late-stage cell proteins, ZapE interacts directly TCA enzymes bacterial two hybrid assays. report direct interactions between succinate dehydrogenase subunit SdhC, FtsN, ZapE. These likely enable communication oxidative machinery UPEC. Moreover, these conserved an K-12 strain. This work suggests there coordination among fundamental pathways regulate overall growth, quiescence, susceptibility. Importance Uropathogenic leading cause urinary tract infections (UTIs). Upon invasion into bladder epithelial cells, establish intracellular reservoirs lead tolerance recurrent UTIs. using system cells ampicillin have decreased by succinyl-CoA limitation. identify global as critical regulators quiescence tolerance. Lastly, components both cycle, this interaction non-pathogenic , establishing novel link metabolism.

Language: Английский

Citations

0
BIOCHEMICAL AND GENETIC MECHANISMS UNDERPINNING ESCHERICHIA COLI CELL CYCLE AND GROWTH TRANSITIONS DOI Creative Commons
Josiah J. Morrison

Published: Jan. 1, 2023

Bacterial cell division and the decision to divide is a process that requires wide variety of factors. In bacterial cells, such as widely studied Escherichia coli, large protein complex, termed Z-ring, assembles at midcell enable division. The Z-ring subject many regulators which govern spatiotemporal positioning ring. made up eukaryotic tubulin homolog FtsZ, polymerizes recruit proteins midcell. FtsZ polymers are tethered cytoplasmic membrane by actin FtsA. Together, FtsA act first step in pathway least 30 septum, 12 essential, constrict one into two identical progeny cells. natural environment, cells not continually dividing they do under laboratory conditions; encounter conditions stress nutrient deprivation, pH fluctuation, oxygen limitation, among others. Some can enter non-replicative, or dormant state, provides them with survival advantages face environmental stresses antibiotic treatment.

Language: Английский

Citations

0