A bacteriophage cocktail delivered in feed significantly reduced Salmonella colonization in challenged broiler chickens

Emerging Microbes & Infections, Journal Year: 2023, Volume and Issue: 12(1)

Published: May 24, 2023

Nontyphoidal Salmonella spp. are a leading cause of human gastrointestinal infections and commonly transmitted via the consumption contaminated meat. To limit spread other food-borne pathogens in food chain, bacteriophage (phage) therapy could be used during rearing or pre-harvest stages animal production. This study was conducted to determine if phage cocktail delivered feed is capable reducing colonization experimentally challenged chickens optimal dose. A total 672 broilers were divided into six treatment groups T1 (no diet unchallenged); T2 (phage 106 PFU/day); T3 (challenged group); T4 105 PFU/day challenged); T5 T6 107 challenged). The liquid added mash with ad libitum access available throughout study. By day 42 (the concluding study), no detected faecal samples collected from group T4. isolated small number pens (3/16) (2/16) at ∼4 × 102 CFU/g. In comparison, 7/16 ∼3 104 Phage all three doses had positive impact on growth performance birds increased weight gains comparison diet. We showed delivering phages effective our highlights offer promising tool target bacterial poultry.

Language: Английский

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High throughput platform technology for rapid target identification in personalized phage therapy

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 11, 2024

Abstract As bacteriophages continue to gain regulatory approval for personalized human therapy against antibiotic-resistant infections, there is a need transformative technologies rapid target identification through multiple, large, decentralized therapeutic phages biobanks. Here, we design high throughput phage screening platform comprised of portable library individual shelf-stable, ready-to-use phages, in all-inclusive solid tablets. Each tablet encapsulates one along with luciferin and luciferase enzyme stabilized sugar matrix pullulan trehalose capable directly detecting phage-mediated adenosine triphosphate (ATP) release ATP bioluminescence reaction upon bacterial cell burst. The composition also enhances desiccation tolerance all components, which should allow easier cheaper international transportation as result, increased accessibility phages. We demonstrate by identifying select multidrug-resistant clinical isolates Pseudomonas aeruginosa , Salmonella enterica Escherichia coli Staphylococcus aureus targets identified within 30-120 min.

Language: Английский

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Effectiveness of newly isolated bacteriophages targeting multidrug-resistant Extraintestinal Pathogenic Escherichia coli strain (TZ1_3) in food preservation and mice health modulation

Food Chemistry, Journal Year: 2025, Volume and Issue: 472, P. 142833 - 142833

Published: Jan. 14, 2025

Language: Английский

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Bacteriophage therapy for multidrug-resistant infections: current technologies and therapeutic approaches

Journal of Clinical Investigation, Journal Year: 2025, Volume and Issue: 135(5)

Published: March 2, 2025

Bacteriophage (phage) therapy has emerged as a promising solution to combat the growing crisis of multidrug-resistant (MDR) infections. There are several international centers actively engaged in implementation phage therapy, and recent case series have reported encouraging success rates patients receiving personalized, compassionate for difficult-to-treat Nonetheless, substantial hurdles remain way more widespread adoption consistent success. This Review offers comprehensive overview current technologies therapeutic approaches. We first delineate common steps development, from bank establishment clinical administration, examine spectrum approaches, personalized fixed cocktails. Using framework conventional drug development pipeline, we then identify critical knowledge gaps areas such cocktail design, formulation, pharmacology, trial design. conclude that, while holds promise, structured pipeline sustained government support crucial MDR

Language: Английский

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Challenges of Studying the Human Virome – Relevant Emerging Technologies

Trends in Microbiology, Journal Year: 2020, Volume and Issue: 29(2), P. 171 - 181

Published: July 1, 2020

Shotgun sequencing bypasses the need for metabarcoding in viromics, although it is prone to high background noises and biases towards double-stranded DNA viruses.Protein-level assembly can be a better tool use on virome data as they predict more protein sequences from complex unknown metagenomes.Using viral discovery methods help resolve full diversity of fraction microbiome data.Culture-independent such viral-tagging used measure phage host range human body. In this review we provide an overview current challenges advances bacteriophage research within growing field viromics. particular, discuss, study perspective, emerging technologies available, their limitations terms de novo discoveries, possible solutions overcome present experimental computational associated with low abundance or RNA. We summarize recent breakthroughs metagenomics assembling tools single-cell analysis, which have potential increase our understanding biology, diversity, interactions both microbial community expect that these future viromics will strong impact how develop phage-based therapeutic approaches.

Language: Английский

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