Critical Review on Two-Stage Anaerobic Digestion with H2 and CH4 Production from Various Wastes

Water, Journal Year: 2024, Volume and Issue: 16(11), P. 1608 - 1608

Published: June 4, 2024

Anaerobic digestion (AD) is a promising method for resource recovery from various wastes. Compared to the conventional single-stage AD process, two-stage process with separate H2 and CH4 production provides higher energy efficiency enhanced operation stability. The stage separation makes it possible apply optimal conditions different functional microorganisms in their respective stages. This review elaborates mechanisms of evaluates recent research trends on this topic. A comprehensive comparison between single- processes made perspective biogas production, organics degradation, recovery, main influence factors are discussed, including substrates, inoculum, parameters, such as pH, temperature, etc. Upgrading technologies assessed. microbial communities treating substrates systems also summarized. Furthermore, future opportunities enhancing application technology highlighted.

Language: Английский

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Bioenergetic Modeling of the Relationship Between Voltage and Electroactive Microbial Biomass Yield for Bioelectrochemical Carbon Dioxide Reduction to Methane

Fermentation, Journal Year: 2025, Volume and Issue: 11(1), P. 40 - 40

Published: Jan. 17, 2025

Optimal product synthesis in bioelectrochemical systems (BESs) requires a comprehensive understanding of the relationship between external voltage and microbial yield. While most studies assume constant growth yields or rely on empirical estimates, this study presents novel thermodynamic model, linking anodic oxidation cathodic carbon dioxide (CO2) reduction to methane (CH4) by growing biofilm. Through integrating theoretical Gibbs free energy calculations, model predicts electron proton transfers for autotrophic methanogen anode-respiring bacteria (ARB) growth, accounting varying applied voltages substrate concentrations. The findings identify an optimal potential −0.3 V vs. standard hydrogen electrode (SHE) maximizing CH4 production under conditions (pH 7, 25 °C, 1 atm) regardless ohmic losses. bridges stoichiometry biofilms, addressing research gaps simulating biofilm simultaneously. Additionally, sensitivity analyses reveal that lower concentrations require more negative than condition stimulate growth. was validated using experimental data, demonstrating reasonable predictions biomass yield different operating multi system. results show higher inputs increase while reducing output due non-optimal voltage. This provides tool optimizing BES performance enhance recovery stability. These insights contribute finding optimum highest efficient CO2 scaling up technology.

Language: Английский

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Stimulation and inhibition of direct interspecies electron transfer mechanisms within methanogenic reactors by adding magnetite and granular actived carbon

Chemical Engineering Journal, Journal Year: 2021, Volume and Issue: 415, P. 128882 - 128882

Published: Feb. 15, 2021

Language: Английский

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Newly discovered Asgard archaea Hermodarchaeota potentially degrade alkanes and aromatics via alkyl/benzyl-succinate synthase and benzoyl-CoA pathway

The ISME Journal, Journal Year: 2021, Volume and Issue: 15(6), P. 1826 - 1843

Published: Jan. 15, 2021

Asgard archaea are widely distributed in anaerobic environments. Previous studies revealed the potential capability of to utilize various organic substrates including proteins, carbohydrates, fatty acids, amino acids and hydrocarbons, suggesting that play an important role sediment carbon cycling. Here, we describe a previously unrecognized archaeal phylum, Hermodarchaeota, affiliated with superphylum. The genomes these were recovered from metagenomes generated mangrove sediments, found encode alkyl/benzyl-succinate synthases their activating enzymes similar those identified alkane-degrading sulfate-reducing bacteria. Hermodarchaeota also potentially involved alkyl-coenzyme A benzoyl-coenzyme oxidation, Wood-Ljungdahl pathway nitrate reduction. These results indicate members this phylum have strictly anaerobically degrade alkanes aromatic compounds, coupling reduction nitrate. By screening Sequence Read Archive, additional genes encoding 16S rRNA analogous metagenomic datasets wide range marine freshwater sediments. findings suggest capable degrading aromatics via formation alkyl/benzyl-substituted succinates ubiquitous

Language: Английский

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Correlation of Key Physiological Properties of Methanosarcina Isolates with Environment of Origin

Applied and Environmental Microbiology, Journal Year: 2021, Volume and Issue: 87(13)

Published: May 17, 2021

It is known that the physiology of

Language: Английский

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Mechanisms of Energy Transduction by Charge Translocating Membrane Proteins

Chemical Reviews, Journal Year: 2021, Volume and Issue: 121(3), P. 1804 - 1844

Published: Jan. 5, 2021

Life relies on the constant exchange of different forms energy, i.e., energy transduction. Therefore, organisms have evolved in a way to be able harvest made available by external sources (such as light or chemical compounds) and convert these into biological useable forms, such transmembrane difference electrochemical potential (Δμ̃). Membrane proteins contribute establishment Δμ̃ coupling exergonic catalytic reactions translocation charges (electrons/ions) across membrane. Irrespectively source consequent type reaction, all charge-translocating follow two molecular mechanisms: direct- indirect-coupling, depending whether translocated charge is involved driving reaction. In this review, we explore mechanisms thoroughly examining types membrane proteins. For each protein, analyze respective reaction thermodynamics, electron transfer/catalytic processes, pathways, ion/substrate stoichiometries.

Language: Английский

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Expanding the phylogenetic distribution of cytochrome b-containing methanogenic archaea sheds light on the evolution of methanogenesis

The ISME Journal, Journal Year: 2022, Volume and Issue: 16(10), P. 2373 - 2387

Published: July 9, 2022

Abstract Methane produced by methanogenic archaea has an important influence on Earth’s changing climate. Methanogenic are phylogenetically diverse and widespread in anoxic environments. These microorganisms can be divided into two subgroups based whether or not they use b-type cytochromes for energy conservation. Methanogens with have a wider substrate range higher growth yields than those without them. To date, methanogens were found exclusively the phylum “Ca. Halobacteriota” (formerly part of Euryarchaeota). Here, we present discovery metagenome-assembled genomes harboring methyl-coenzyme M reductase genes reconstructed from mesophilic sediments, together previously reported thermophilic Methylarchaeum tengchongensis”, representing novel archaeal order, designated Methylarchaeales”, Thermoproteota TACK superphylum). contain required methyl-reducing methanogenesis Wood-Ljundahl pathway. Importantly, genus Methanotowutia” Methylarchaeales” encode cytochrome b-containing heterodisulfide (HdrDE) methanophenazine-reducing hydrogenase complex that similar gene arrangements to Methanosarcinales. Our results indicate members conserve via membrane-bound electron transport chains. Phylogenetic amalgamated likelihood estimation analyses transfer complexes likely evolved before diversification early Archean Eon. Surveys public sequence databases suggest lineage globally distributed sediments may players methane cycle.

Language: Английский

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Evolving understanding of rumen methanogen ecophysiology

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 6, 2023

Production of methane by methanogenic archaea, or methanogens, in the rumen ruminants is a thermodynamic necessity for microbial conversion feed to volatile fatty acids, which are essential nutrients animals. On other hand, greenhouse gas and its production causes energy loss animal. Accordingly, there ongoing efforts toward developing effective strategies mitigating emissions from ruminant livestock that require detailed understanding diversity ecophysiology methanogens. Rumen methanogens evolved free-living autotrophic ancestors through genome streamlining involving gene acquisition. The process yielded an oligotrophic lifestyle, metabolically efficient ecologically adapted descendants. This specialization poses serious challenges obtaining axenic cultures consequently, information on their physiological properties remains most part inferred those non-rumen representatives. review presents current knowledge metabolic contributions enteric production. It also identifies respective critical gaps need be filled aiding mitigate emission operations at same time increasing productivity this agriculture sector.

Language: Английский

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A comprehensive review on methane’s dual role: effects in climate change and potential as a carbon–neutral energy source

Environmental Science and Pollution Research, Journal Year: 2023, Volume and Issue: 31(7), P. 10379 - 10394

Published: Oct. 26, 2023

Language: Английский

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Biofouling and biocorrosion by microbiota from a marine oil pipeline: A metagenomic and proteomic approach

Journal of environmental chemical engineering, Journal Year: 2023, Volume and Issue: 11(2), P. 109413 - 109413

Published: Feb. 1, 2023

Biofouling and biocorrosion in oil pipelines are serious problems capable of causing expensive, irreparable losses. For a better understanding processes, several lines evidence required, not least experimental considerations that emulate natural conditions inside pipelines. Herein, an omics approach is used to unveil the microbial involvement deterioration electrochemical process on X52 steel with Shewanella (SW), pipeline microbiota (AK), mixed culture (AK5), latter two obtained from sludge pig cleaning Gulf Mexico. Shotgun metagenomics showed diverse communities at species level sludge: bacteria up 112, 34 archaea, 9 fungi, 23 viruses. In AK most abundant taxa were Petrotogaceae Petrotoga (31.16%) Methanomicrobiaceae (48.51%). AK5 Methylobacterium (49%) (19.49%). Concerning fungi viruses, respectively: Clavariaceae (43.26%), Picciniaceae (35.59), Onygenaceae (3.77%), Siphoviridae (46.8%), Myoviridae (33.50%), Podoviridae (7.36%) AK5. Proteomics revealed 32,284 proteins AK, 13,664 AK5, 7 SW. Expressed oxidoreductases, lyases, nitrogenases, metalloproteases, hydrolases associated process, for instance, electron transport, sulfur oxidation, nitrate reduction, biofilm formation, oxygen reduction. addition functional annotation metagenomes KEGG pathways, through Multigenomic Entropy Based Score (MEBS) metabolic functions evaluated explaining relation energy transfer processes biogeochemical cycles. The was analyzed by X-ray diffraction (XRD), fluorescence (XRF), Mössbauer spectroscopy suggesting biofouling processes. Regarding assessments, scanning microscopy (SEM) Energy Dispersive Spectroscopy (EDX) coupled SEM, SW biofilms behaved differently. Three mechanisms proposed.

Language: Английский

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