Chlamydiae as symbionts of photosynthetic dinoflagellates DOI Creative Commons
Justin Maire, Astrid Collingro, Kshitij Tandon

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 18, 2023

Abstract Chlamydiae are ubiquitous intracellular bacteria and infect a wide diversity of eukaryotes, including mammals. However, chlamydiae have never been reported to photosynthetic organisms. Here, we describe novel chlamydial genus species, Candidatus Algichlamydia australiensis ( A. thereafter), capable infecting the dinoflagellate Cladocopium sp. (originally isolated from scleractinian coral). was confirmed be by fluorescence in situ hybridization confocal laser scanning microscopy, temporally stable at population level monitoring its relative abundance across four weeks host growth. Using combination short- long-read sequencing, recovered high-quality (completeness 91.73% contamination 0.27%) metagenome-assembled genome . Phylogenetic analyses show that this taxon represents new species within Simkaniaceae family. possesses all hallmark genes for chlamydiae-host interactions, complete type III secretion system. In addition, IV system is encoded on plasmid has previously observed only three other species. Twenty orthologous groups unique , one which structurally similar protein known Cyanobacteria Archaeplastida involved thylakoid biogenesis maintenance, hinting potential interactions with chloroplasts cells. Despite being itself symbiont cnidarians, meta-analysis 12,009 cnidarian 16S rRNA gene metabarcoding samples returned five sequences, suggesting does not associate cnidarians. Our study shows symbionts first organism harbor chlamydiae, thereby expanding breadth hosts providing contribution discussion around role establishment primary plastid.

Language: Английский

Chlamydiae as symbionts of photosynthetic dinoflagellates DOI Creative Commons
Justin Maire, Astrid Collingro, Kshitij Tandon

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 18, 2023

Abstract Chlamydiae are ubiquitous intracellular bacteria and infect a wide diversity of eukaryotes, including mammals. However, chlamydiae have never been reported to photosynthetic organisms. Here, we describe novel chlamydial genus species, Candidatus Algichlamydia australiensis ( A. thereafter), capable infecting the dinoflagellate Cladocopium sp. (originally isolated from scleractinian coral). was confirmed be by fluorescence in situ hybridization confocal laser scanning microscopy, temporally stable at population level monitoring its relative abundance across four weeks host growth. Using combination short- long-read sequencing, recovered high-quality (completeness 91.73% contamination 0.27%) metagenome-assembled genome . Phylogenetic analyses show that this taxon represents new species within Simkaniaceae family. possesses all hallmark genes for chlamydiae-host interactions, complete type III secretion system. In addition, IV system is encoded on plasmid has previously observed only three other species. Twenty orthologous groups unique , one which structurally similar protein known Cyanobacteria Archaeplastida involved thylakoid biogenesis maintenance, hinting potential interactions with chloroplasts cells. Despite being itself symbiont cnidarians, meta-analysis 12,009 cnidarian 16S rRNA gene metabarcoding samples returned five sequences, suggesting does not associate cnidarians. Our study shows symbionts first organism harbor chlamydiae, thereby expanding breadth hosts providing contribution discussion around role establishment primary plastid.

Language: Английский

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