Multispecies emergence of dual bla KPC/NDM carbapenemase-producing Enterobacterales recovered from invasive infections in Chile DOI Creative Commons
Ana María Quesille-Villalobos, Camila Solar, José R W Martínez

et al.

Antimicrobial Agents and Chemotherapy, Journal Year: 2024, Volume and Issue: 69(1)

Published: Dec. 5, 2024

Carbapenemase-producing carbapenem-resistant Enterobacterales (CP-CRE) represent a significant global threat. The emergence of dual CP-CRE is particularly alarming, as they can potentially compromise the efficacy newer antibiotics, further decreasing therapeutic alternatives. Herein, we report multiple species recovered from invasive infections in Chile that simultaneously harbor blaKPC and blaNDM provide an in-depth genomic characterization these worrisome pathogens. We collected (CRE) isolates over 4-year period, across 11 healthcare centers Chile. Bacterial presence carbapenemase genes were confirmed using MALDI-TOF PCR assays, respectively. Antimicrobial susceptibility testing was conducted through disk diffusion broth microdilution methods. Dual subjected to short- long-read whole genome sequencing perform detailed mobile genetic elements harboring enzymes. From total 1,335 CRE isolates, observed increase prevalence CP-CRE, 11% 2019 38% 2022. A recovered, all them blaNDM. Species corresponded Escherichia coli (n = 6), Klebsiella pneumoniae 2), oxytoca Citrobacter freundii 1). exhibited resistance tested β-lactams except for cefiderocol. encoding located on independent plasmids. Platforms diverse included IncN, IncF, IncFIB In contrast, blaNDM-7 only found fairly conserved IncX3 rapid Chile, alongside with bacterial co-harboring blaKPC-2/3 blaNDM-7, underscores critical public health challenge. Our data suggest dissemination predominantly facilitated by plasmids, whereas spread involved plasmid backbones. Active surveillance monitoring are inform policy curtail highly resistant

Language: Английский

Rapid emergence, transmission, and evolution of KPC and NDM coproducing carbapenem-resistant Klebsiella pneumoniae DOI
Jiayang Li,

Wenqi Wu,

Hao Wu

et al.

Microbiological Research, Journal Year: 2025, Volume and Issue: 293, P. 128049 - 128049

Published: Jan. 5, 2025

Language: Английский

Citations

1
Enhanced invasion and survival of antibiotic- resistant Klebsiella pneumoniae pathotypes in host cells and strain-specific replication in blood DOI Creative Commons

Kathleen Klaper,

Yvonne Pfeifer,

Lena Heinrich

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 14, 2025

Background Klebsiella pneumoniae is one of the most important opportunistic pathogens causing healthcare-associated and community-acquired infections worldwide. In recent years, increase in antibiotic resistance caused by hypervirulent K. poses great public health concerns. this study, host-pathogen interactions different strains human animal origins were analyzed microbiological, cell-biological immunological experiments. Methods vitro infection experiments using representatives pathotypes various epithelial macrophage cell lines executed analyzing adhesion, invasion intracellular replication. Experimental conditions involved normoxia hypoxia. Furthermore, survival growth further isolates expressing defined siderophores blood (platelet concentrates, serum) was investigated. All done triplicate statistically significant differences determined. Results Significant adhesion capability, phagocytosis replication measured between pathotypes. Especially, ESBL-producing demonstrated increased host macrophages. A strong cytotoxic effect on intestinal cells observed for . The results from our investigations behavior platelets serum showed that and/or an enlarged capsule are not essential factors proliferation (hypervirulent) components. Conclusion Our revealed new insights into representing pathovars clonal lineages infectious contexts hosts. While a clear limitation study limited strain set used both as potential host, step better understanding pathogenicity its properties stages colonization infection. When developed further, these may offer novel approaches future therapeutics including “anti-virulence strategies”.

Language: Английский

Citations

0
The Challenge of Treating Infections Caused by Metallo‐β‐Lactamase–Producing Gram-Negative Bacteria: A Narrative Review DOI Creative Commons
Carmen Hidalgo‐Tenorio, Germán Bou,

Antonio Oliver

et al.

Drugs, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 28, 2024

Gram-negative multidrug-resistant (MDR) bacteria, including Enterobacterales, Acinetobacter baumannii, and Pseudomonas aeruginosa, pose a significant challenge in clinical practice. Infections caused by metallo-β-lactamase (MBL)–producing organisms, particular, require careful consideration due to their complexity varied prevalence, given that the microbiological diagnosis of these pathogens is intricate compounded challenges assessing efficacy anti-MBL antimicrobials. We discuss both established new approaches treatment MBL–producing infections, focusing on 3 strategies: colistin; recently approved combination aztreonam with avibactam (or ceftazidime/avibactam); cefiderocol. Despite its activity against various pathogens, colistin limited resistance mechanisms, while nephrotoxicity acute renal injury call for dosing monitoring Aztreonam combined avibactam/ceftazidime if plus not available) exhibits potent pathogens. Cefiderocol monotherapy effective wide range MBL producers, favorable outcomes have been observed trials case series. After examining scientific evidence management infections we developed comprehensive algorithm guide therapeutic decision making. recommend reserving as last-resort option MDR infections. aztreonam/avibactam represent options In P. aeruginosa enzymes difficult-to-treat resistance, cefiderocol preferred option. Further research needed optimize strategies minimize resistance.

Language: Английский

Citations

2
Contributions of Long-Read Sequencing for the Detection of Antimicrobial Resistance DOI Creative Commons
Roberto Sierra, Mélanie Roch,

Milo Moraz

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(9), P. 730 - 730

Published: Aug. 28, 2024

Background. In the context of increasing antimicrobial resistance (AMR), whole-genome sequencing (WGS) bacteria is considered a highly accurate and comprehensive surveillance method for detecting tracking spread resistant pathogens. Two primary technologies exist: short-read (50–300 base pairs) long-read (thousands pairs). The former, based on Illumina platforms (ISPs), provides extensive coverage high accuracy single nucleotide polymorphisms (SNPs) small insertions/deletions, but limited by its read length. latter, such as Oxford Nanopore Technologies (ONT), enables assembly genomes, particularly those with repetitive regions structural variants, although has historically been lower. Results. We performed head-to-head comparison these techniques to sequence K. pneumoniae VS17 isolate, focusing blaNDM gene alleles in program. Discrepancies between ISP (blaNDM-4 allele identified) ONT (blaNDM-1 blaNDM-5 were observed. Conjugation assays Sanger sequencing, used gold standard, confirmed validity results. This study demonstrates importance or hybrid assemblies carbapenemase identification highlights limitations short reads duplications multiple alleles. Conclusions. this proof-of-concept study, we conclude that recent technology may outperform standard Such information crucial given rising prevalence strains producing carbapenemases, especially WGS increasingly epidemiological infection control.

Language: Английский

Citations

1
Case report of cefiderocol-resistant hypervirulent Klebsiella pneumoniae with CirA deficiency and co-production of KPC-2 and SHV-12 DOI
Peng Lan, Ye Lu, Wei‐Chao Liao

et al.

Clinical Microbiology and Infection, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

Citations

0
Multispecies emergence of dual bla KPC/NDM carbapenemase-producing Enterobacterales recovered from invasive infections in Chile DOI Creative Commons
Ana María Quesille-Villalobos, Camila Solar, José R W Martínez

et al.

Antimicrobial Agents and Chemotherapy, Journal Year: 2024, Volume and Issue: 69(1)

Published: Dec. 5, 2024

Carbapenemase-producing carbapenem-resistant Enterobacterales (CP-CRE) represent a significant global threat. The emergence of dual CP-CRE is particularly alarming, as they can potentially compromise the efficacy newer antibiotics, further decreasing therapeutic alternatives. Herein, we report multiple species recovered from invasive infections in Chile that simultaneously harbor blaKPC and blaNDM provide an in-depth genomic characterization these worrisome pathogens. We collected (CRE) isolates over 4-year period, across 11 healthcare centers Chile. Bacterial presence carbapenemase genes were confirmed using MALDI-TOF PCR assays, respectively. Antimicrobial susceptibility testing was conducted through disk diffusion broth microdilution methods. Dual subjected to short- long-read whole genome sequencing perform detailed mobile genetic elements harboring enzymes. From total 1,335 CRE isolates, observed increase prevalence CP-CRE, 11% 2019 38% 2022. A recovered, all them blaNDM. Species corresponded Escherichia coli (n = 6), Klebsiella pneumoniae 2), oxytoca Citrobacter freundii 1). exhibited resistance tested β-lactams except for cefiderocol. encoding located on independent plasmids. Platforms diverse included IncN, IncF, IncFIB In contrast, blaNDM-7 only found fairly conserved IncX3 rapid Chile, alongside with bacterial co-harboring blaKPC-2/3 blaNDM-7, underscores critical public health challenge. Our data suggest dissemination predominantly facilitated by plasmids, whereas spread involved plasmid backbones. Active surveillance monitoring are inform policy curtail highly resistant

Language: Английский

Citations

0