An Insight into the Presence of Antimicrobial Resistance Genes in Opportunistic Pathogenic Bacteria Isolated from Farm-Reared Crickets DOI Creative Commons
Teresita d. J. Bello Gonzalez,

Betty van Gelderen,

Frank Harders

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 391 - 391

Published: Feb. 11, 2025

To support the role of insects as sustainable feed and food ingredients, evaluating their potential microbiological risk safety is crucial. In this study, we investigated presence antimicrobial resistance (AMR) genes in selected live opportunistic pathogenic bacteria isolated during rearing process from clinically healthy farm-reared crickets. Molecular analysis was performed by wholegenome sequencing a total 14 these bacterial strains, 7 house crickets (Acheta domesticus) banded (Gryllodes sigillatus), belonging to Enterobacteriaceae, Staphylococcaceae, Enterococcaceae, Bacillaceae families. The β-lactam AMR (blaOXY2-6, blaACT-16, blaSHV variants) were most predominant identified, mainly Enterobacteriaceae strains association with fosfomycin (fosA) oqxAB efflux pump complexes. addition, blaZ mecA detected Bacillus cereus Mammaliicoccus sciuri both insect species. Genetic mobile elements including IncFIA, IncFIB, IncHI1A, IncHI1B, rep13, Col3M-like plasmids Klebsiella pneumoniae, Enterobacter hormaechei, Staphylococcus arlettae, B. cereus, respectively. results indicate that, not only final product but also insect-rearing process, microbial control, regarding genes, essential for effectively decreasing between cricket batches within environment terms related chain.

Language: Английский

An Insight into the Presence of Antimicrobial Resistance Genes in Opportunistic Pathogenic Bacteria Isolated from Farm-Reared Crickets DOI Creative Commons
Teresita d. J. Bello Gonzalez,

Betty van Gelderen,

Frank Harders

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 391 - 391

Published: Feb. 11, 2025

To support the role of insects as sustainable feed and food ingredients, evaluating their potential microbiological risk safety is crucial. In this study, we investigated presence antimicrobial resistance (AMR) genes in selected live opportunistic pathogenic bacteria isolated during rearing process from clinically healthy farm-reared crickets. Molecular analysis was performed by wholegenome sequencing a total 14 these bacterial strains, 7 house crickets (Acheta domesticus) banded (Gryllodes sigillatus), belonging to Enterobacteriaceae, Staphylococcaceae, Enterococcaceae, Bacillaceae families. The β-lactam AMR (blaOXY2-6, blaACT-16, blaSHV variants) were most predominant identified, mainly Enterobacteriaceae strains association with fosfomycin (fosA) oqxAB efflux pump complexes. addition, blaZ mecA detected Bacillus cereus Mammaliicoccus sciuri both insect species. Genetic mobile elements including IncFIA, IncFIB, IncHI1A, IncHI1B, rep13, Col3M-like plasmids Klebsiella pneumoniae, Enterobacter hormaechei, Staphylococcus arlettae, B. cereus, respectively. results indicate that, not only final product but also insect-rearing process, microbial control, regarding genes, essential for effectively decreasing between cricket batches within environment terms related chain.

Language: Английский

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