Harnessing sequencing data for porcine reproductive and respiratory syndrome virus (PRRSV): tracking genetic evolution dynamics and emerging sequences in US swine industry DOI Creative Commons

Srijita Chandra,

Guilherme Cezar,

Kinath Rupasinghe

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: March 6, 2025

Porcine reproductive and respiratory syndrome virus (PRRSV) is the most important swine pathogen affecting United States of America (USA), leading to significant economic losses. Despite advances in diagnostic testing, there remains a gap understanding genetic evolution PRRSV, especially tracking emergence novel sequences their spread across different regions production stages. This research addresses this by developing systematic methodology for directly collecting analyzing PRRSV ORF5 from veterinary laboratories. The study aimed identify trends among collected emerging integrating nucleotide sequence data with metadata, providing critical insights into geographic distribution, specimens, age groups, lineages, variants, restriction fragment length polymorphism (RFLP) patterns. As December 2024, database housed 115,643 sequences. Sublineages 1B, 1A, 1H, 1C.5 were major wild-type detected over time, whereas vaccine-like strains comprised mostly sublineages 5A 8A. A detection system was implemented, categorizing based on similarity thresholds, ambiguities, criteria, identifying 167 period between 2010 only three had continued field forming clusters detection. analysis these highlighted trends, including dominance grow-finish animals origin high number detections sublineage 5A. Production sites located states largest inventory have contributed frequent new strains. Additionally, development web-based tool provides end users capability search similar query sequence, macroepidemiological information features support management control. Real-time sequencing informs producers veterinarians any upcoming findings are intended enhance current surveillance efforts more effective strategies managing outbreaks, ultimately safeguarding animal health, sustainability industry, contributing national food sovereignty through pork-derived products.

Language: Английский

Repeat offenders: PRRSV-2 clinical re-breaks from a whole genome perspective DOI
Julia P. Baker, Albert Rovira, Kimberly VanderWaal

et al.

Veterinary Microbiology, Journal Year: 2025, Volume and Issue: 302, P. 110411 - 110411

Published: Jan. 29, 2025

Language: Английский

Citations

0
Harnessing sequencing data for porcine reproductive and respiratory syndrome virus (PRRSV): tracking genetic evolution dynamics and emerging sequences in US swine industry DOI Creative Commons

Srijita Chandra,

Guilherme Cezar,

Kinath Rupasinghe

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: March 6, 2025

Porcine reproductive and respiratory syndrome virus (PRRSV) is the most important swine pathogen affecting United States of America (USA), leading to significant economic losses. Despite advances in diagnostic testing, there remains a gap understanding genetic evolution PRRSV, especially tracking emergence novel sequences their spread across different regions production stages. This research addresses this by developing systematic methodology for directly collecting analyzing PRRSV ORF5 from veterinary laboratories. The study aimed identify trends among collected emerging integrating nucleotide sequence data with metadata, providing critical insights into geographic distribution, specimens, age groups, lineages, variants, restriction fragment length polymorphism (RFLP) patterns. As December 2024, database housed 115,643 sequences. Sublineages 1B, 1A, 1H, 1C.5 were major wild-type detected over time, whereas vaccine-like strains comprised mostly sublineages 5A 8A. A detection system was implemented, categorizing based on similarity thresholds, ambiguities, criteria, identifying 167 period between 2010 only three had continued field forming clusters detection. analysis these highlighted trends, including dominance grow-finish animals origin high number detections sublineage 5A. Production sites located states largest inventory have contributed frequent new strains. Additionally, development web-based tool provides end users capability search similar query sequence, macroepidemiological information features support management control. Real-time sequencing informs producers veterinarians any upcoming findings are intended enhance current surveillance efforts more effective strategies managing outbreaks, ultimately safeguarding animal health, sustainability industry, contributing national food sovereignty through pork-derived products.

Language: Английский

Citations

0