Diversity in chemical subunits and linkages: a key molecular determinant of microbial richness, microbiota interactions, and substrate utilization DOI Creative Commons
Hugh C. McCullough, Hyun‐Seob Song, Jennifer M. Auchtung

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Dietary fibers play a significant role in shaping the composition and function of microbial communities human colon. Our understanding specific chemical traits dietary that influence diversity, interactions, remains limited. Toward filling this knowledge gap, we developed novel measure, termed Chemical Subunits Linkages (CheSL) Shannon to characterize effects carbohydrate complexity on fecal bacteria cultured vitro under controlled, continuous flow conditions using media systematically varied composition. analysis revealed CheSL diversity demonstrated strong Pearson correlation with richness across multiple samples study designs. Additionally, observed higher scores exhibited greater peptide utilization more connected, reproducible structures computationally inferred interaction networks. Taken together, these findings demonstrate can be useful tool quantify metabolic potential, interactions. Furthermore, our work highlights how robust stable community data generated by engineering structure. These studies provide valuable framework for future research interactions their potential impacts host health.IMPORTANCEFor adult gut microbiota, strongly correlates positive health outcomes. This is likely due increased resilience results from functional redundancy occur within diverse communities. While previous have shown microbiota function, lack complete mechanistic differences are functionally impact diversity. To address need, measure describes complexity. Using were able correlate changes alterations interspecies Overall, analyses new perspectives optimization strategies improve health.

Language: Английский

A designed synthetic microbiota provides insight to community function in Clostridioides difficile resistance DOI Creative Commons
Shuchang Tian, Min Soo Kim, Jingcheng Zhao

et al.

Cell Host & Microbe, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Clostridioides difficile, a major cause of antibiotic-associated diarrhea, is suppressed by the gut microbiome, but precise mechanisms are not fully described. Through meta-analysis 12 human studies, we designed synthetic fecal microbiota transplant (sFMT1) reconstructing microbial networks negatively associated with C. difficile colonization. This lab-built 37-strain consortium formed functional community suppressing in vitro and animal models. Using sFMT1 as tractable model system, find that bile acid 7α-dehydroxylation determinant efficacy while one strain performing Stickland fermentation-a pathway competitive nutrient utilization-is both necessary sufficient for suppression replicating gnotobiotic mouse model. Our data illustrate significance competition generalizable approach to interrogating complex function through robust methods leverage publicly available sequencing data.

Language: Английский

Citations

0
Diversity in chemical subunits and linkages: a key molecular determinant of microbial richness, microbiota interactions, and substrate utilization DOI Creative Commons
Hugh C. McCullough, Hyun‐Seob Song, Jennifer M. Auchtung

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Dietary fibers play a significant role in shaping the composition and function of microbial communities human colon. Our understanding specific chemical traits dietary that influence diversity, interactions, remains limited. Toward filling this knowledge gap, we developed novel measure, termed Chemical Subunits Linkages (CheSL) Shannon to characterize effects carbohydrate complexity on fecal bacteria cultured vitro under controlled, continuous flow conditions using media systematically varied composition. analysis revealed CheSL diversity demonstrated strong Pearson correlation with richness across multiple samples study designs. Additionally, observed higher scores exhibited greater peptide utilization more connected, reproducible structures computationally inferred interaction networks. Taken together, these findings demonstrate can be useful tool quantify metabolic potential, interactions. Furthermore, our work highlights how robust stable community data generated by engineering structure. These studies provide valuable framework for future research interactions their potential impacts host health.IMPORTANCEFor adult gut microbiota, strongly correlates positive health outcomes. This is likely due increased resilience results from functional redundancy occur within diverse communities. While previous have shown microbiota function, lack complete mechanistic differences are functionally impact diversity. To address need, measure describes complexity. Using were able correlate changes alterations interspecies Overall, analyses new perspectives optimization strategies improve health.

Language: Английский

Citations

0