Recombinant serralysin metalloproteases D enhances the intracellular replication of infectious bovine rhinotracheitis virus DOI Creative Commons

Longfei Yan,

Yanran Li,

Jiancheng Qi

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: May 9, 2025

Infectious bovine rhinotracheitis virus (IBRV) and Serratia marcescens co-infection are commonly observed in the respiratory tract of cattle subjected to diseases. However, potential effects proteases from on IBRV infection remain poorly understood. In this study, we investigated role recombinant serralysin-like protease D (rSPD) modulating Madin-Darby kidney (MDBK) cells. Our findings demonstrate that rSPD enhances replication exacerbates cytopathic MDBK Quantification gB gene copy numbers using fluorescence quantification PCR (FQ-PCR) revealed promotes viral during intracellular stage, without affecting adsorption, entry, or directly interacting with particles. The transcriptomic analysis further demonstrated suppresses innate immune responses while amplifying inflammatory pathways IBRV-infected Gene Ontology (GO) KEGG enrichment identified significant differentially expressed genes (DEGs) key signaling pathways, including JAK–STAT, NOD-like receptor, Toll-like TNF, NF-κB, MAPK pathways. Notably, downregulated associated immunity, such as ISG15 , OAS2 IFIT1 IFIT2 IFIT3 MX1 RSAD2 MX2 SAA3 DDX58 IFI44 IRF1 suggesting host antiviral defenses. Conversely, upregulated involved response, IL-6 IL-8 CCL2 CX3CL1 CCL3 CXCL3 indicating may exacerbate cellular damage promote by inducing excessive responses. These provide novel insights into interplay between bacterial infections, highlighting exacerbating pathogenesis offering a foundation for research therapeutic strategies targeting bacterial-viral interactions.

Language: Английский

Recombinant serralysin metalloproteases D enhances the intracellular replication of infectious bovine rhinotracheitis virus DOI Creative Commons

Longfei Yan,

Yanran Li,

Jiancheng Qi

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: May 9, 2025

Infectious bovine rhinotracheitis virus (IBRV) and Serratia marcescens co-infection are commonly observed in the respiratory tract of cattle subjected to diseases. However, potential effects proteases from on IBRV infection remain poorly understood. In this study, we investigated role recombinant serralysin-like protease D (rSPD) modulating Madin-Darby kidney (MDBK) cells. Our findings demonstrate that rSPD enhances replication exacerbates cytopathic MDBK Quantification gB gene copy numbers using fluorescence quantification PCR (FQ-PCR) revealed promotes viral during intracellular stage, without affecting adsorption, entry, or directly interacting with particles. The transcriptomic analysis further demonstrated suppresses innate immune responses while amplifying inflammatory pathways IBRV-infected Gene Ontology (GO) KEGG enrichment identified significant differentially expressed genes (DEGs) key signaling pathways, including JAK–STAT, NOD-like receptor, Toll-like TNF, NF-κB, MAPK pathways. Notably, downregulated associated immunity, such as ISG15 , OAS2 IFIT1 IFIT2 IFIT3 MX1 RSAD2 MX2 SAA3 DDX58 IFI44 IRF1 suggesting host antiviral defenses. Conversely, upregulated involved response, IL-6 IL-8 CCL2 CX3CL1 CCL3 CXCL3 indicating may exacerbate cellular damage promote by inducing excessive responses. These provide novel insights into interplay between bacterial infections, highlighting exacerbating pathogenesis offering a foundation for research therapeutic strategies targeting bacterial-viral interactions.

Language: Английский

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