Metagenome-Assembled Genomes (MAGs): Advances, Challenges, and Ecological Insights DOI Creative Commons
Salvador Mirete, Mercedes Sánchez‐Costa, Jorge Díaz-Rullo

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(5), P. 985 - 985

Published: April 25, 2025

Metagenome-assembled genomes (MAGs) have revolutionized microbial ecology by enabling the genome-resolved study of uncultured microorganisms directly from environmental samples. By leveraging high-throughput sequencing, advanced assembly algorithms, and genome binning techniques, researchers can reconstruct without need for cultivation. These methodological advances expanded known diversity, revealing novel taxa metabolic pathways involved in key biogeochemical cycles, including carbon, nitrogen, sulfur transformations. MAG-based studies identified lineages form Archaea Bacteria responsible methane oxidation, carbon sequestration marine sediments, ammonia metabolism, highlighting their critical roles ecosystem stability. From a sustainability perspective, MAGs provide essential insights climate change mitigation, sustainable agriculture, bioremediation. The ability to characterize communities diverse environments, soil, aquatic ecosystems, extreme habitats, enhances biodiversity conservation supports development microbial-based management strategies. Despite these advancements, challenges such as biases, incomplete reconstructions, taxonomic uncertainties persist. Continued improvements sequencing technologies, hybrid approaches, multi-omics integration will further refine analyses. As methodologies advance, remain cornerstone understanding contributions global processes developing interventions resilience.

Language: Английский

Metagenome‐Assembled Genomes for Oligotrophic Nitrifiers From a Mountainous Gravelbed Floodplain DOI
Anna Rasmussen, Bradley B. Tolar, John Bargar

et al.

Environmental Microbiology, Journal Year: 2025, Volume and Issue: 27(3)

Published: March 1, 2025

ABSTRACT Riparian floodplains are important regions for biogeochemical cycling, including nitrogen. Here, we present MAGs from nitrifying microorganisms, ammonia‐oxidising archaea (AOA) and comammox bacteria Slate River (SR) floodplain sediments (Crested Butte, CO, US). Additionally, explore potential nitrite‐oxidising (NOB) the Nitrospirales . AOA diversity in SR is lower than observed other western US Nitrosotalea ‐like lineages such as genus TA‐20 dominant AOA. No (AOB) were recovered. Microorganisms Palsa‐1315 (clade B comammox) most abundant ammonia‐oxidizers sediments. Established NOB conspicuously absent; however, recovered uncultured of NS‐4 family ( ) Nitrospiraceae that propose putative NOB. Nitrite oxidation may be carried out by organisms sister to established Nitrospira based on genomic content clades. Nitrifier harbour genes using alternative sources ammonia, urea, cyanate, biuret, triuret nitriles. The therefore appears a low ammonia flux environment selects oligotrophic nitrifiers.

Language: Английский

Citations

0
Metagenome-Assembled Genomes (MAGs): Advances, Challenges, and Ecological Insights DOI Creative Commons
Salvador Mirete, Mercedes Sánchez‐Costa, Jorge Díaz-Rullo

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(5), P. 985 - 985

Published: April 25, 2025

Metagenome-assembled genomes (MAGs) have revolutionized microbial ecology by enabling the genome-resolved study of uncultured microorganisms directly from environmental samples. By leveraging high-throughput sequencing, advanced assembly algorithms, and genome binning techniques, researchers can reconstruct without need for cultivation. These methodological advances expanded known diversity, revealing novel taxa metabolic pathways involved in key biogeochemical cycles, including carbon, nitrogen, sulfur transformations. MAG-based studies identified lineages form Archaea Bacteria responsible methane oxidation, carbon sequestration marine sediments, ammonia metabolism, highlighting their critical roles ecosystem stability. From a sustainability perspective, MAGs provide essential insights climate change mitigation, sustainable agriculture, bioremediation. The ability to characterize communities diverse environments, soil, aquatic ecosystems, extreme habitats, enhances biodiversity conservation supports development microbial-based management strategies. Despite these advancements, challenges such as biases, incomplete reconstructions, taxonomic uncertainties persist. Continued improvements sequencing technologies, hybrid approaches, multi-omics integration will further refine analyses. As methodologies advance, remain cornerstone understanding contributions global processes developing interventions resilience.

Language: Английский

Citations

0