Clinical and Therapeutic Potential of Bacillus subtilis Antimicrobial Peptides DOI
Sajid Iqbal,

Mehwish Malik,

Bushra Mustafa

et al.

IntechOpen eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

The bioactive peptides produced by Bacillus subtilis have garnered significant attention for their diverse bioactivities and promising therapeutic applications. These exhibit broad-spectrum activity against bacterial, viral, fungal, parasitic infections, making them potent candidates treating drug-resistant pathogens. Further, these exhibited anticancer selectively targeting cancer cells, inducing apoptosis, inhibiting tumor cell migration invasion. Beyond direct antimicrobial properties, B. also possess immunomodulatory effects, enhancing host immune responses reducing inflammation. This dual role in both pathogen elimination regulation expands potential, particularly use chronic infections wound healing. To prevent from hydrolysis, biomaterial liposome delivery systems been designed to improve the stability bioavailability of peptides. key challenge peptide-based drug discovery is accurate prediction peptide functionality stability, given complexity interactions with biological targets. Machine learning algorithms can be employed design optimized properties accelerate identification novel enhanced efficacy specificity. Overall, this chapter demonstrates mechanisms action, applications,

Language: Английский

Enhancement of gut barrier integrity by a Bacillus subtilis secreted metabolite through the GADD45A‐Wnt/β‐catenin pathway DOI Creative Commons
Shiqi Liu, Peiran Cai, Wenjing You

et al.

iMeta, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 23, 2025

Inflammatory bowel disease (IBD) represents a significant challenge to global health, characterized by intestinal inflammation, impaired barrier function, and dysbiosis, with limited therapeutic options. In this study, we isolated novel strain of Bacillus subtilis (B. subtilis) observed promising effects in protecting against disruption the gut barrier. Our findings indicate that enhancement function is primarily attributed its metabolites. We identified metabolite, 2-hydroxy-4-methylpentanoic acid (HMP), derived from B. subtilis, significantly improved function. also show growth arrest DNA damage 45A (GADD45A) key regulator mucosal integrity, which activated HMP subsequently activates downstream Wnt/β-catenin pathway. potentially contribute development probiotics-derived metabolites or targeted "postbiotics" as therapeutics for treatment prevention IBD other diseases associated dysfunction.

Language: Английский

Citations

4
Exploring Bacillus velezensis in a biomedical context: a systematic review DOI Creative Commons
Eduarda Guimarães Sousa, Gabriela Munis Campos,

Ludmila Silva Quaresma

et al.

Academia molecular biology and genomics., Journal Year: 2025, Volume and Issue: 2(1)

Published: March 26, 2025

Bacillus velezensis is associated with producing secondary metabolites antifungal, anti-inflammatory, antagonistic, and antimicrobial activities that have multiple applications in the food industry, agriculture, farming. This study aims to review current knowledge highlight biomedical potential of as a mammalian probiotic supported by vivo vitro research compile existing knowledge. All information this was searched Dimensions, Scopus, PubMed, Web Science databases on 2023 Dec 10. The articles were selected according Preferred Reporting Items for Systematic Reviews Meta-Analyses (PRISMA) method scientometric analysis. A total 1585 records identified after screening process. Of these, 151 used analysis, 13 data analysis based studies. literature revealed great B. its relevance scientific research, especially recent years, thanks advances genome sequencing search alternative antibiotics. production such surfactin, bacilysin, fengycin growing interest industry human health. It also has due antagonistic activity, lack antibiotic resistance, immunomodulatory activity. However, optimization use better understanding cultivation methods gene expression under variable environmental conditions are crucial. Future should focus refining applying techniques maximize these bioactive compounds exploit full various applications.

Language: Английский

Citations

1
Bacillus subtilis Utilizes Decarboxylated S-Adenosylmethionine for the Biosynthesis of Tandem Aminopropylated Microcin C, a Potent Inhibitor of Bacterial Aspartyl-tRNA Synthetase DOI
Alexey Kulikovsky,

Eldar Yagmurov,

Anastasiia Grigoreva

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

The biosynthetic pathways of natural products involve unusual biochemical reactions catalyzed by unique enzymes. Aminopropylation, although apparently simple, is an extremely rare modification outside polyamine biosynthesis. canonical pathway used in the biosynthesis peptide-adenylate antibiotic microcin C E. coli (Eco-McC) entails alkylation S-adenosyl-methionine-derived 3-amino-3-carboxypropyl group adenylate moiety and subsequent decarboxylation to yield bioactive aminopropylated compound. Here, we report structure a new member family antibiotics, Bsu-McC, produced Bacillus subtilis MG27, which employs alternative aminopropylation pathway. Like Eco-McC, Bsu-McC consists peptide that facilitates prodrug import into susceptible bacteria warhead, nonhydrolyzable modified isoasparaginyl-adenylate, which, when released cytoplasm, binds aspartyl-tRNA synthetase (AspRS) inhibiting translation. In contrast whose warhead carries single aminopropyl attached phosphate decorated with tandem two groups. Our silico docking AspRS-tRNA complex suggests groups form extended interactions enzyme tRNA, stabilizing enzyme–inhibitor complex. We show results 32-fold increase biological activity peptidyl-adenylate. also B. adopted for homologous 3-aminopropyltransferases utilize decarboxylated S-adenosylmethionine as substrate. Additionally, alters social behavior producer strain, resulting sharp decrease their ability biofilms.

Language: Английский

Citations

0
Multiple probiotic effects of Ligilactobacillus murinus strain S27 on large yellow croaker (Larimichthys crocea): Promoting growth, improving intestinal health, and enhancing resistance against Pseudomonas plecoglossicida DOI
Zhixin Lin, Jianping Zeng,

Yu Feng Zhai

et al.

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742657 - 742657

Published: April 1, 2025

Language: Английский

Citations

0
Insights into biofilm-mediated mechanisms driving last-resort antibiotic resistance in clinical ESKAPE pathogens DOI
Christina Shook Cheng Chong, Yin Yin Lau, Paul A.M. Michels

et al.

Critical Reviews in Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 26

Published: March 17, 2025

The rise of antibiotic-resistant bacteria poses a grave threat to global health, with the ESKAPE pathogens, which comprise Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp. being among most notorious. World Health Organization has reserved group last-resort antibiotics for treating multidrug-resistant bacterial infections, including those caused by pathogens. This situation calls comprehensive understanding resistance mechanisms as it threatens public health hinder progress toward Sustainable Development Goal (SDG) 3: Good Well-being. present article reviews mechanisms, focusing on emerging mutations in particularly against antibiotics, describes role biofilm formation It discusses latest therapeutic advances, use antimicrobial peptides CRISPR-Cas systems, modulation quorum sensing iron homeostasis, offer promising strategies countering resistance. integration CRISPR-based tools biofilm-targeted approaches provides potential framework managing infections. By highlighting spread current approaches, review aims contribute significantly advancing our combatting this pressing challenge.

Language: Английский

Citations

0
Disarming Staphylococcus aureus: Review of Strategies Combating This Resilient Pathogen by Targeting Its Virulence DOI Creative Commons
Abdelaziz Touati, Nasir A. Ibrahim, Takfarinas Idres

et al.

Pathogens, Journal Year: 2025, Volume and Issue: 14(4), P. 386 - 386

Published: April 15, 2025

Staphylococcus aureus is a formidable pathogen notorious for its antibiotic resistance and diverse virulence mechanisms, including toxin production, biofilm formation, immune evasion. This article explores innovative anti-virulence strategies to disarm S. by targeting critical factors without exerting bactericidal pressure. Key approaches include inhibiting adhesion neutralizing toxins, disrupting quorum sensing (e.g., Agr system inhibitors), blocking iron acquisition pathways. Additionally, interventions two-component regulatory systems are highlighted. While promising, challenges such as strain variability, resilience, pharmacokinetic limitations, evolution underscore the need combination therapies advanced formulations. Integrating with traditional antibiotics host-directed offers sustainable solution combat multidrug-resistant aureus, particularly methicillin-resistant strains (MRSA), mitigate global public health crisis.

Language: Английский

Citations

0
The exometabolome as a hidden driver of bacterial virulence and pathogenesis DOI
Saurabh Chugh, Fabien Létisse, Olivier Neyrolles

et al.

Trends in Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

Citations

1
Host Defense Peptides: Exploiting an Innate Immune Component Against Infectious Diseases and Cancer DOI
Taiwo Scholes Adewole, Oladiran Boniface Oladokun, Adenike Kuku

et al.

International Journal of Peptide Research and Therapeutics, Journal Year: 2024, Volume and Issue: 30(6)

Published: Oct. 5, 2024

Language: Английский

Citations

0
Clinical and Therapeutic Potential of Bacillus subtilis Antimicrobial Peptides DOI
Sajid Iqbal,

Mehwish Malik,

Bushra Mustafa

et al.

IntechOpen eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

The bioactive peptides produced by Bacillus subtilis have garnered significant attention for their diverse bioactivities and promising therapeutic applications. These exhibit broad-spectrum activity against bacterial, viral, fungal, parasitic infections, making them potent candidates treating drug-resistant pathogens. Further, these exhibited anticancer selectively targeting cancer cells, inducing apoptosis, inhibiting tumor cell migration invasion. Beyond direct antimicrobial properties, B. also possess immunomodulatory effects, enhancing host immune responses reducing inflammation. This dual role in both pathogen elimination regulation expands potential, particularly use chronic infections wound healing. To prevent from hydrolysis, biomaterial liposome delivery systems been designed to improve the stability bioavailability of peptides. key challenge peptide-based drug discovery is accurate prediction peptide functionality stability, given complexity interactions with biological targets. Machine learning algorithms can be employed design optimized properties accelerate identification novel enhanced efficacy specificity. Overall, this chapter demonstrates mechanisms action, applications,

Language: Английский

Citations

0