Evolution of genotypic and phenotypic diversity in multispecies biofilms DOI Open Access
Cristina I. Amador,

Sofia Zoe Moscovitz,

Lorrie Maccario

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 8, 2023

Abstract The fitness and adaptability of bacteria in microbial communities can be influenced by the interactions among community partners, as well specific spatial organisation. Here, we investigated evolutionary dynamics Bacillus thuringiensis response to presence or absence structure bacterial interspecies interactions. During evolution experiments, a distinct phenotypic variant B. frequently occurred, irrespective conditions tested: planktonic vs. biofilm monospecies mixed species. Remarkably, selection significantly favored this over its ancestor settings when coexisting with Pseudomonas defluvii brenneri , co-isolated from wastewater facility. Interestingly, evolved phenotype displayed lower biomass species biofilms, shorter generation time, lacked sporulation compared ancestor. strains displaying variation contained mutations regulator spo0A that initiates but also de-represses expression matrix determinants other Bacilli. Consistently, proteomics revealed reduced abundance TasA variant, major component species, while higher was found co-culture P. . Our results indicate within biofilms not only promote diversification select for altered traits such production. Moreover, favoured settings, suggesting facilitate co-existence. These findings could impact applications where are utilized consortia, plant growth promoters biopesticides.

Language: Английский

Microbial fungicides can positively affect aubergine photosynthetic properties, soil enzyme activity and microbial community structure DOI Creative Commons

Longxue Wei,

Jinying Zhu,

Dongbo Zhao

et al.

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e17620 - e17620

Published: June 28, 2024

This study examined the effects of microbial agents on enzyme activity, community construction and potential functions inter-root soil aubergine (

Language: Английский

Citations

1
Agro Active Potential of Bacillus subtilis PE7 against Didymella bryoniae (Auersw.), the Causal Agent of Gummy Stem Blight of Cucumis melo DOI Creative Commons

Seo Kyoung Jeong,

Seong Eun Han,

Prabhakaran Vasantha‐Srinivasan

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(8), P. 1691 - 1691

Published: Aug. 16, 2024

Microbial agents such as the

Language: Английский

Citations

1
(p)ppGpp interacts with TrmE and regulates the PcoI/PcoR quorum-sensing system of Pseudomonas fluorescens 2P241 DOI Creative Commons

Ruijing Shang,

Shihai Liang,

Qing Yan

et al.

Journal of Integrative Agriculture, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

Citations

0
The extracytoplasmic sigma factor SigX supports biofilm formation and increases biocontrol efficacy in Bacillus velezensis 118 DOI

Yanfei Cai,

Huan Tao,

Hualiang Pi

et al.

Published: Oct. 3, 2024

Abstract Utilizing plant growth promoting rhizobacteria (PGPR) to combat pathogens and enhance crop production is an environmentally friendly sustainable approach. The biocontrol activity of PGPR depends on their ability colonize roots synthesize antimicrobial compounds that inhibit pathogens. However, the regulatory mechanisms underlying these processes remain unclear. In this study, we isolated characterized Bacillus velezensis isolate 118, a soil isolate exhibits potent against Fusarium wilt banana. Deletion sigX, extracytoplasmic function (ECF) sigma factor previously implicated in controlling biofilm architecture in B. subtilis, reduced efficacy. B. 118 sigXmutant displayed reduced formation but had only minor defect swarming motility negligible impact lipopeptide production. These findings highlight importance important for root colonization effectiveness Bacillus spp. as agents phytopathogens.

Language: Английский

Citations

0
Thermal Plasticity and Evolutionary Constraints in Bacillus: Implications for Climate Change Adaptation DOI Creative Commons

Enrique Hurtado-Bautista,

África Islas-Robles,

Gabriel Moreno‐Hagelsieb

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(12), P. 1088 - 1088

Published: Dec. 23, 2024

The ongoing rise in global temperatures poses significant challenges to ecosystems, particularly impacting bacterial communities that are central biogeochemical cycles. resilience of wild mesophilic bacteria temperature increases 2–4 °C remains poorly understood. In this study, we conducted experimental evolution on six Bacillus strains from two lineages (Bacillus cereus and subtilis) examine their thermal adaptation strategies. We exposed the gradually increasing assess plasticity, focusing genetic mechanisms underlying adaptation. While B. subtilis improved growth at highly critical temperatures, only one increased its niche 4 above natural range. This finding is concerning given climate change projections. exhibited higher mutation rates but were not able grow while required fewer changes increase heat tolerance, indicating distinct adaptive observed convergent five evolved lines, with mutations genes involved c-di-AMP synthesis, which crucial for potassium transport, implicating chemical messenger first time tolerance. These insights highlight vulnerability underscore importance background shaping

Language: Английский

Citations

0
Evolution of genotypic and phenotypic diversity in multispecies biofilms DOI Open Access
Cristina I. Amador,

Sofia Zoe Moscovitz,

Lorrie Maccario

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 8, 2023

Abstract The fitness and adaptability of bacteria in microbial communities can be influenced by the interactions among community partners, as well specific spatial organisation. Here, we investigated evolutionary dynamics Bacillus thuringiensis response to presence or absence structure bacterial interspecies interactions. During evolution experiments, a distinct phenotypic variant B. frequently occurred, irrespective conditions tested: planktonic vs. biofilm monospecies mixed species. Remarkably, selection significantly favored this over its ancestor settings when coexisting with Pseudomonas defluvii brenneri , co-isolated from wastewater facility. Interestingly, evolved phenotype displayed lower biomass species biofilms, shorter generation time, lacked sporulation compared ancestor. strains displaying variation contained mutations regulator spo0A that initiates but also de-represses expression matrix determinants other Bacilli. Consistently, proteomics revealed reduced abundance TasA variant, major component species, while higher was found co-culture P. . Our results indicate within biofilms not only promote diversification select for altered traits such production. Moreover, favoured settings, suggesting facilitate co-existence. These findings could impact applications where are utilized consortia, plant growth promoters biopesticides.

Language: Английский

Citations

0